干旱区生态环境知识资源中心(Arid): 固相萃取-毛细管区带电泳法同时测定豆制品中4种碱性染料

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	固相萃取-毛细管区带电泳法同时测定豆制品中4种碱性染料
其他题名	Simultaneous Determination of Four Basic Dyes in Soybean Products by Capillary Zone Electrophoresis Combined with Solid Phase Extraction
	张伟; 陈艳; 何丰瑞
来源期刊	理化检验. B, 化学分册
ISSN	1001-4020
出版年	2017
卷号	53 期号:9 页码:1020-1024
中文摘要	采用固相萃取结合毛细管区带电泳法同时测定豆制品中碱性橙2、碱性橙21、碱性橙22和碱性嫩黄O。2.00 0 g样品经50 mmol · L ~(-1)乙酸铵溶液5.00 mL超声提取两次,提取液经Oasis HLB固相萃取柱净化,用氨水-乙腈(5 + 95)溶液进行洗脱,洗脱液经氮气吹干,残渣用乙腈-水(25 + 75)溶液溶解并定容至1.00 mL后进行毛细管区带电泳分离,分离电压为20 kV,分离温度为30 °C,运行缓冲溶液为含20%(体积分数)乙腈的40 mmol · L~(-1)磷酸二氢钾溶液(pH 2.5),采用紫外检测器,检测波长为210 nm。4种碱性染料在7 min内达到基线分离,线性范围均为1.25~50 mg · L~(-1),检出限(3S/N)在0.5~0.7 mg · kg~(-1)之间。加标回收率在73.6%~93.1%之间,测定值的相对标准偏差(n=6)小于10%。
英文摘要	A method of capillary zone electrophoresis couple with solid phase extraction was applied to the simultaneous determination of basic orange 2,basic orange 21,basic orange 22 and auramine O in soybean products. The sample (2.000 g) was ultrasonically extracted twice with 5.00 mL of 50 mmol · L~(-1) ammonium acetate solution,the extract was purified on Oasis HLB solid phase extraction column and the 4 target dyes were eluted with ammonia-acetonitrile (5 + 95) solution. The eluent was driedup by nitrogen blowing and the residue was dissolved and diluted to 1.00 mL with acetonitrile-H_2O (25 + 75) solution. The target dyes were separated by capillary zone electrophoresis at 30 ℃ and 20 kV with 40 mmol · L~(-1) potassium dihydrogen phosphate solution containing 20% (phi) acetonitrile as running buffer solution (pH 2.5) and detected with a UV detector at the wavelength of 210 nm. Separation of the 4 dyes from baseline was achieved within 7 min. The linearity ranges of the four basic dyes were all 1.25 50 mg · L~(-1) and detection limits (3S/N) were in the range of 0.5 0.7 mg · kg~(-1). Recovery rates obtained by standard addition method were 73.693.1 %,and RSDs (n = 6) were less than 10%.
中文关键词	毛细管区带电泳法 ; 固相萃取 ; 碱性染料 ; 豆制品
英文关键词	capillary zone electrophoresis solid phase extraction basic dye soybean product
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6069793
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236260
作者单位	西安市疾病预防控制中心, 西安, 陕西 710054, 中国
推荐引用方式 GB/T 7714	张伟,陈艳,何丰瑞. 固相萃取-毛细管区带电泳法同时测定豆制品中4种碱性染料[J],2017,53(9):1020-1024.
APA	张伟,陈艳,&何丰瑞.(2017).固相萃取-毛细管区带电泳法同时测定豆制品中4种碱性染料.理化检验. B, 化学分册,53(9),1020-1024.
MLA	张伟,et al."固相萃取-毛细管区带电泳法同时测定豆制品中4种碱性染料".理化检验. B, 化学分册 53.9(2017):1020-1024.