干旱区生态环境知识资源中心(Arid): 苦豆子液泡膜Na~+/H~+逆向运输蛋白基因SaNHX1的克隆与生物信息学分析

Arid

	苦豆子液泡膜Na~+/H~+逆向运输蛋白基因SaNHX1的克隆与生物信息学分析
其他题名	Cloning and Bioinformatics Analysis of SaNHX1 in Na~+/H~+ Antiporter Gene in Vacuole Membrane of Sophora alopecuroides
	柏丹阳 1; 王彦芹 2; 李志军 2
来源期刊	基因组学与应用生物学
ISSN	1674-568X
出版年	2017
卷号	36 期号:2 页码:727-733
中文摘要	苦豆子(Sophora alopecuroides L.)因其具有很强的耐盐、耐旱、耐高温等功能广泛分布于干旱荒漠区。研究报道植物液泡膜Na~+/H~+逆向运输蛋白对植株耐盐性及耐旱性等具有重要作用。本研究首先对苦豆子种子进行萌发处理,采用改良的异硫氰酸胍法提取苦豆子的总RNA,利用逆转录试剂盒获得苦豆子的cDNA,通过同源克隆法获得了苦豆子液泡膜Na~+/H~+逆向运输蛋白基因的cDNA全长,并对其氨基酸序列及可能的功能进行了生物信息学分析。结果显示,所获得的基因编码548个氨基酸,与豆科植物大豆GlNHX1的一致性最高为86%;二级结构预测显示其具有10个跨膜结构,不含信号肽;对其3D结构预测显示该蛋白是一个富含alpha螺旋的桶装蛋白质。这些预测结果表明本研究所克隆的基因为苦豆子液泡膜逆向运输蛋白Na~+/H~+基因,这将为荒漠植物苦豆子抗逆基因资源的发掘利用奠定基础。
英文摘要	Sophora alopecuroides is widely distributed in arid desert area because of its strong salt tolerance, drought tolerance and high-temperature resistance. Studies have shown that Na ~+/H ~+ antiporter protein in plant vacuole membrane has an important impact on salt and drought tolerance of plants. In this research, we first germinated Sophora alopecuroides seeds, from which we extracted the total RNA of Sophora alopecuroides by using improved guanidinium thiocyanate method, and then we got its cDNA and full length cDNA of Na ~+/H ~+ antiporter gene in plant vacuole membrane through reverse transcription kits and homology cloning respectively, and finally we analyzed its amino acid sequence and possible function by bioinformatics technology. The result showed that the consistency between the obtained gene encoding 548 amino acids and leguminous plant soybean reached the maximum of 86%; The secondary structure prediction indicated that it had 10 transmembrane structures and did not contain signal peptide; Its 3D structure prediction showed that this protein was a bottled protein, which was rich in alpha helix. These predicted results showed that the cloned gene in this research was the gene of Na~+/H~+ antiporter protein in plant vacuole membrane, which would lay the foundation for the exploration and utilization of stress-tolerance genes resources of Sophora alopecuroides.
中文关键词	苦豆子 ; Na~+/H~+逆向运输蛋白 ; 生物信息学分析
英文关键词	Sophora alopecuroides Na~+/H~+ antiporter protein Bioinformatics analysis
语种	中文
国家	中国
收录类别	CSCD
WOS类目	BIOLOGY
WOS研究方向	Life Sciences & Biomedicine - Other Topics
CSCD记录号	CSCD:5958299
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236214
作者单位	1.塔里木大学,生命科学学院, 阿拉尔, 新疆 843300, 中国; 2.塔里木大学,生命科学学院, 塔里木盆地生物资源保护利用兵团重点实验室;;新疆省部共建国家重点实验室培训基地, 阿拉尔, 新疆 843300, 中国
推荐引用方式 GB/T 7714	柏丹阳,王彦芹,李志军. 苦豆子液泡膜Na~+/H~+逆向运输蛋白基因SaNHX1的克隆与生物信息学分析[J],2017,36(2):727-733.
APA	柏丹阳,王彦芹,&李志军.(2017).苦豆子液泡膜Na~+/H~+逆向运输蛋白基因SaNHX1的克隆与生物信息学分析.基因组学与应用生物学,36(2),727-733.
MLA	柏丹阳,et al."苦豆子液泡膜Na~+/H~+逆向运输蛋白基因SaNHX1的克隆与生物信息学分析".基因组学与应用生物学 36.2(2017):727-733.