干旱区生态环境知识资源中心(Arid): 荒漠昆虫小胸鳖甲水通道蛋白基因MpAQP1克隆及低温对其表达的影响

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	荒漠昆虫小胸鳖甲水通道蛋白基因MpAQP1克隆及低温对其表达的影响
其他题名	Cloning of an aquaporin gene ( MpAQP1) from the desert beetle Microdera punctipennis and influence of low temperature on its expression
	李耀华; 刘小宁; 马纪
来源期刊	环境昆虫学报
ISSN	1674-0858
出版年	2017
卷号	39 期号:6 页码:1326-1333
中文摘要	水通道蛋白( aquaporin,AQP)参与细胞的水分平衡与运输,与生物的抗逆性相关,但AQP在昆虫耐寒性中的作用仅有少量报道。为了检测昆虫AQP基因的序列特征及其表达是否与低温相关,本研究从荒漠甲虫小胸鳖甲Microdera punctipennis中克隆获得AQP的开放阅读框( ORF)序列,命名为MpAQP1 ( GenBank登录号: KX129951) 。生物信息学分析表明,MpAQP1的ORF长度为813 bp,编码270个氨基酸,分子量为28.59 kDa,理论等电点为7.73。MpAQP1属于萤火虫内嵌蛋白( PRIP)家族,具有6个跨膜结构域和5个螺旋环,含有高度保守的天冬酰胺-脯氨酸-丙氨酸基序( NPA基序) 。实时荧光定量PCR检测MpAQP1在低温下的表达谱发现,4℃低温处理1 h,MpAQP1开始上调表达,5 h达到最高峰,为对照组的34.39倍。研究表明MpAQP1为冷响应基因,水通道蛋白可能参与小胸鳖甲低温下的渗透调节而发挥耐寒性功能。
英文摘要	Aquaporin ( AQP) involves in water balance and transport in cells,thus it is related to organisms’ stress resistance.However,studies on the function of AQP in insect cold resistance are rare.To detect whether the expression of AQP gene is induced by low temperature,we firstly cloned the Open Reading Frame ( ORF) sequence of the aquaporin gene from the desert beetle Microdera punctipennis,and named it as MpAQP1 ( GenBank accession number: KX129951 ).Bioinformatics analysis showed that MpAQP1 ORF is 813 bp encoding 270 amino acids with a molecular weight of 28.59 kDa,the theoretical isoelectric point is 7.73.MpAQP1 is a member of Pyrocoelia rufa integral protein ( PRIP) family.MpAQP1 exhibited a typical structure with six transmembrane domains,five helix loops and two highly conserved asparagine-proline-alanine motifs ( NPA motifs).Real time quantitative PCR results showed that the expression of MpAQP1 was up-regulated after the insect was treated at 4℃ for 1 h,and reached peak at 5 h,which was 34.39 times of the control group.Our study indicated that MpAQP1 was a cold responsive gene,which may play role in improving cold tolerance of M.punctipennis by involving in osmoregulation.
中文关键词	水通道蛋白 ; 耐寒性 ; 渗透调节 ; 低温表达
英文关键词	Aquaporin cold resistance osmoregulation expression at low temperature
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENTOMOLOGY
WOS研究方向	Entomology
CSCD记录号	CSCD:6145373
来源机构	新疆大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236207
作者单位	新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 乌鲁木齐, 新疆 830046, 中国
推荐引用方式 GB/T 7714	李耀华,刘小宁,马纪. 荒漠昆虫小胸鳖甲水通道蛋白基因MpAQP1克隆及低温对其表达的影响[J]. 新疆大学,2017,39(6):1326-1333.
APA	李耀华,刘小宁,&马纪.(2017).荒漠昆虫小胸鳖甲水通道蛋白基因MpAQP1克隆及低温对其表达的影响.环境昆虫学报,39(6),1326-1333.
MLA	李耀华,et al."荒漠昆虫小胸鳖甲水通道蛋白基因MpAQP1克隆及低温对其表达的影响".环境昆虫学报 39.6(2017):1326-1333.