干旱区生态环境知识资源中心(Arid): 猪场废水中24种抗生素同时检测方法优化

Arid

	猪场废水中24种抗生素同时检测方法优化
其他题名	Optimization of an analytical method for the simultaneous determination of 24 antibiotics in swine wastewater
	柴玉峰 1; 冯玉启 2; 张玉秀 1; 陈梅雪 3; 王瑞 3; 柳蒙蒙 3; 魏源送 3
来源期刊	环境化学
ISSN	0254-6108
出版年	2017
卷号	36 期号:10 页码:2147-2154
中文摘要	采用优化固相萃取-液相色谱-串联质谱同步检测养猪废水中24种抗生素的.水样用1 mol·L~(-1)盐酸调节pH值至3.5-4.0,流经Oasis HLB固相萃取柱富集后再用甲醇和乙酸乙酯(V: V = 1: 1)进行洗脱.以Agilent eclipse plus C_(18)色谱柱为分离柱,以0.1%甲酸水溶液和乙腈为流动相进行梯度洗脱,采用电喷雾正离子源,多反应监测模式检测.24种抗生素的检出限(S /N= 3)在0.03-3.00 mug·L~(-1)之间.进一步以不同养猪场废水进行实际样品测定,四环素类、大环内酯类、beta-内酰胺类、喹诺酮类、磺胺类等5大类抗生素均有检出,土霉素、替米考星和磺胺甲嘧啶等3种抗生素为主要污染抗生素,最高浓度可达147.10、107.83、100.20 mug·L~(-1),磺胺甲噻二唑及去甲基金霉素均未检出.本研究建立了快速准确的同步分析5大类24种抗生素的分析方法.
英文摘要	An optimized method employing solid phase extraction-liquid chromatography-tandem mass spectrometry was established for the simultaneous determination of 24 antibiotics in swine wastewater. After adjusting pH to 3.5-4.0 by using 1 mol·L~(-1) hydrochloric acid,the samples were concentrated and collected after flowing through an Oasis HLB solid-phase extraction column,and were eluted with methanol and ethyl acetate (V:V = 1:1). The gradient elution was carried out with 0.1% (V:V) formic acid solution and acetonitrile as the mobile phase by using the Agilent eclipse plus C_(18) column,and the detection was performed under the monitoring mode of the electrospray ionization source multiple reaction. The limit of detection (S /N = 3) of the 24 antibiotics was between 0.03-3.00 mug·L~(-1). Tetracyclines,macrolides,beta-lactams,quinolones and sulfonamides were all detected by this method in the swine wastewater samples. As the three main antibiotics, oxytetracycline,tilmicosin and sulfamethoxazole were found to have a maximum concentration of 147.10,107.83 and 100.20 mug·L~(-1), respectively whereas sulfadoxadiazole and dimethyl chlortetracycline were not detected. The rapid and accurate detection of 24 antibiotics in 5 major classes was established.
中文关键词	兽用抗生素 ; 高效液相色谱-质谱联用法 ; 固相萃取 ; 养殖废水
英文关键词	veterinary antibiotics LC-MS /MS solid phase extraction(SPE) swine wastewater
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENVIRONMENTAL SCIENCES
WOS研究方向	Environmental Sciences & Ecology
CSCD记录号	CSCD:6100241
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236182
作者单位	1.中国矿业大学(北京)化学与环境工程学院, 北京 100083, 中国; 2.北京建筑大学环境与能源工程学院, 北京 100044, 中国; 3.中国科学院生态环境研究中心, 北京 100085, 中国
推荐引用方式 GB/T 7714	柴玉峰,冯玉启,张玉秀,等. 猪场废水中24种抗生素同时检测方法优化[J],2017,36(10):2147-2154.
APA	柴玉峰.,冯玉启.,张玉秀.,陈梅雪.,王瑞.,...&魏源送.(2017).猪场废水中24种抗生素同时检测方法优化.环境化学,36(10),2147-2154.
MLA	柴玉峰,et al."猪场废水中24种抗生素同时检测方法优化".环境化学 36.10(2017):2147-2154.