干旱区生态环境知识资源中心(Arid): 超高效液相色谱串联质谱法同时测定城市污水处理厂污泥中12种抗生素

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	超高效液相色谱串联质谱法同时测定城市污水处理厂污泥中12种抗生素
其他题名	Simultaneous determination of 12 antibiotics in sewage sludge by ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry
	宋淑敏; 张翔宇; 周佳虹; 李茹莹
来源期刊	环境化学
ISSN	0254-6108
出版年	2017
卷号	36 期号:9 页码:1923-1931
中文摘要	建立了一种固相萃取-超高效液相色谱-串联质谱法同时测定污泥中4类12种抗生素的方法.采用SAX串联Oasis HLB固相萃取柱净化富集污泥样品中的抗生素,选定pH 3、洗脱体积8 mL作为最佳前处理条件.采用超高效液相色谱-串联质谱检测抗生素样品,以0.3%的甲酸和乙腈作为流动相,经梯度洗脱进行分离,在多反应检测模式下进行测定.经测定,污泥样品中磺胺类抗生素、脱水红霉素和罗红霉素平均回收率为50%80%,其余抗生素的平均回收率为82%119%,12种抗生素在污泥中的定量限为0.001 0.008 mug·kg~(-1) dw.并用该法对天津市某污水处理厂污泥样品进行检测,证明该方法可满足实际检测要求.
英文摘要	A method for the determination of 12 antibiotics in sewage sludge was developed using solid-phase extraction and ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (SPE-UPLC-MS /MS). The antibiotics in sludge samples were enriched and purified by SAX and Oasis HLB solid-phase extraction columns. The optimal pretreatment conditions of pH 3 and 8 mL elution volume were selected. All antibiotics were detected by UPLC-MS /MS in a multiple reaction monitoring mode,separated by gradient elution with the mobile phase of 0.3% formic acid and acetonitrile. Results show that the average recovery efficiencies of sulfonamide antibiotics,erythromycin and roxithromycin in sludge samples were 50%80%,and the average recovery efficiencies of the other antibiotics were 82%119%. The quantitative limit of the 12 antibiotics was 0.0010.008 mug·kg~(-1) dry weight in sludge. The method was utilized for the sludge samples from a wastewater treatment plant in Tianjin,suggesting this method meets the practical detection application.
中文关键词	抗生素 ; 固相萃取 ; 超高效液相色谱串联质谱 ; 污泥
英文关键词	antibiotic solid-phase extraction UPLC-MS /MS sludge
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6078078
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/236181
作者单位	天津大学环境科学与工程学院, 天津 300350, 中国
推荐引用方式 GB/T 7714	宋淑敏,张翔宇,周佳虹,等. 超高效液相色谱串联质谱法同时测定城市污水处理厂污泥中12种抗生素[J],2017,36(9):1923-1931.
APA	宋淑敏,张翔宇,周佳虹,&李茹莹.(2017).超高效液相色谱串联质谱法同时测定城市污水处理厂污泥中12种抗生素.环境化学,36(9),1923-1931.
MLA	宋淑敏,et al."超高效液相色谱串联质谱法同时测定城市污水处理厂污泥中12种抗生素".环境化学 36.9(2017):1923-1931.