干旱区生态环境知识资源中心(Arid): 胡杨F-box基因克隆和功能分析

Arid

	胡杨F-box基因克隆和功能分析
其他题名	Identification and Functional Analysis of F-box Gene from Populus Euphratica
	任逸秋 1; 贾会霞 2; 郭英华 2; 徐向东 2; 李建波 2; 卢孟柱 2; 樊军锋 3; 胡建军 2
来源期刊	分子植物育种
ISSN	1672-416X
出版年	2017
卷号	15 期号:5 页码:1655-1662
中文摘要	F-box蛋白在泛素-蛋白酶体途径中能够特异识别底物蛋白,调节多种激素的信号转导途径,并响应多种非生物胁迫,参与植物抗逆途径。胡杨(Populus euphratica)是中国西北沙漠唯一能成林的高大乔木树种,对盐碱、干旱、极端温度等抵抗能力较强,是研究林木抗逆机理的候选模式树种。本研究对胡杨耐盐性状关联分析获得的F-box基因进行克隆、表达特性分析、亚细胞定位和转化胡杨原生质体的耐盐性测定,旨在研究胡杨F-box基因的功能特性。胡杨基因全长1 086 bp,编码361个氨基酸,含有一个F-box domain和一个F-box associated domain。qRT-PCR表明胡杨F-box基因在各组织中均有表达,无组织特异性,其表达受盐、高温、低温、干旱和ABA胁迫诱导。PEG介导的胡杨原生质体瞬时表达发现F-box蛋白定位于细胞核上,流式细胞术证实过表达F-box基因能够提高胡杨原生质体的耐盐性。研究初步揭示了胡杨F-box基因的表达特性和耐盐性,为进一步解析胡杨F-box基因的功能及胡杨抗逆分子机理提供帮助。
英文摘要	F-box protein could specifically identify the substrate protein in the ubiquitin-proteasome pathway and regulate the signal transduction pathways of hormones. Moreover, F-box gene responses to a variety of abiotic stresses and involves in stress-tolerant pathway in plants. Populus euphratica, a only forest species in the desert region of northwest China, has a strong resistance to salt, drought, extreme temperatures, etc. Thus, it had been considered as a candidate model tree species for the study of mechanism of stress resistance. In this study, the gene cloning, expression characteristics analysis, subcellular localization and salt resistance analysis of F-box gene that obtained by associated analysis for salt-tolerance traits of P. euphratica were performed to search its function characteristics. F-box gene of P. euphratica contained 1 086 nucleotides, which coded 361 amino acids containing F-box domain and F-box associated domain. The results of qRT-PCR showed that F-box gene was expressed in various tissues, without tissue specificity. Its expression was induced by the salt, high temperature, low temperature, drought, and ABA stresses. Transient expressing of P. euphratica protoplast mediated by PEG showed F-box protein was located in nucleus, and it enhanced the salt resistance of protoplast. The preliminary results of this study revealed the expression characteristics and salt resistance of F-box gene from P. euphratica, and provided a reference for further analysis of F-box gene function and molecular mechanism with resistance to stress in P. euphratica.
中文关键词	亚细胞定位 ; 表达特性 ; 原生质体转化 ; 耐盐性
英文关键词	F-box F-box Expression characteristic Subcellular localization Protoplast transformation Salt-resistance
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:6001131
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235866
作者单位	1.西北农林科技大学林学院, 林木遗传育种国家重点实验室;;国家林业局林木培育重点实验室, 杨凌, 712100; 2.中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室;;国家林业局林木培育重点实验室, 北京 100091, 中国; 3.西北农林科技大学林学院, 杨凌, 712100
推荐引用方式 GB/T 7714	任逸秋,贾会霞,郭英华,等. 胡杨F-box基因克隆和功能分析[J]. 西北农林科技大学,2017,15(5):1655-1662.
APA	任逸秋.,贾会霞.,郭英华.,徐向东.,李建波.,...&胡建军.(2017).胡杨F-box基因克隆和功能分析.分子植物育种,15(5),1655-1662.
MLA	任逸秋,et al."胡杨F-box基因克隆和功能分析".分子植物育种 15.5(2017):1655-1662.