干旱区生态环境知识资源中心(Arid): QuEChERS-超高效液相色谱-串联质谱法测定坚果中38种农药残留

Arid

	QuEChERS-超高效液相色谱-串联质谱法测定坚果中38种农药残留
其他题名	Determination of 38 Kinds of Pesticide Residues in Nuts by QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
	董亚蕾; 刘文婧; 曹进; 王钢力
来源期刊	分析化学
ISSN	0253-3820
出版年	2017
卷号	45 期号:9 页码:1397-1404
中文摘要	建立了检测4种坚果(花生、杏仁、腰果、核桃)中38种农药残留的QuEChERS-超高效液相色谱-串联质谱(UPLC-MS/MS)方法。样品均质后,用乙腈进行提取,经PSA和C_(18)净化后,采用Oasis PRiME HLB固相萃取柱进一步净化,UPLC-MS/MS分析。对样品前处理和色谱方法进行了优化。在多重反应监测(MRM)模式下进行质谱分析,外标法定量。38种农药的检出限范围(S/N=3)为0.01~10 mug/kg,定量限(S/N=10)为0.05~20 mug/kg,线性关系良好(r>0.991)。4种坚果中农药的平均加标回收率为51.0%~126.0%,相对标准偏差均小于20%。此方法灵敏、准确、有效,可用于坚果类食品中多种农药残留的同时测定。
英文摘要	A multi-residue analysis method was developed for the determination of 38 kinds of pesticides in nuts ( almonds,peanuts,cashew nuts and walnuts) by QuEChERS-ultra-high performance liquid chromatography-tandem mass spectrometry. The pesticide residues were extracted with acetonitrile. The extract was cleaned up with PSA, C_(18) and Oasis PRiME HLB,and then analyzed by UPLC-MS/MS with multiple reaction monitoring (MRM) mode. External standard method was employed to quantify. The limits of detection (LODs,S/N = 3) of this method were between 0. 01 and 10 mug/kg,and the limits of quantitation (LOQs, S/N=10) were between 0. 05-20 mug/kg. All of the tested pesticides showed good linear relationship (r>0. 991). The practical samples were determined at three spiked levels and the average recoveries were between 51. 0% and 126. 0%. The RSDs were less than 20%. This method was simple, sensitive and accurate,and could be used for the routine analysis of pesticide residues in nuts.
中文关键词	超高效液相色谱-串联质谱 ; 农药残留 ; 坚果
英文关键词	QuEChERS Ultra-high performance liquid chromatography-tandem mass spectrometry QuEChERS Multi-pesticide residues Nuts
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:6067766
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235858
作者单位	中国食品药品检定研究院, 北京 100050, 中国
推荐引用方式 GB/T 7714	董亚蕾,刘文婧,曹进,等. QuEChERS-超高效液相色谱-串联质谱法测定坚果中38种农药残留[J],2017,45(9):1397-1404.
APA	董亚蕾,刘文婧,曹进,&王钢力.(2017).QuEChERS-超高效液相色谱-串联质谱法测定坚果中38种农药残留.分析化学,45(9),1397-1404.
MLA	董亚蕾,et al."QuEChERS-超高效液相色谱-串联质谱法测定坚果中38种农药残留".分析化学 45.9(2017):1397-1404.