干旱区生态环境知识资源中心(Arid): 通过式固相萃取净化/液相色谱- 串联质谱法快速检测水产品中11种青霉素残留

Arid

	通过式固相萃取净化/液相色谱- 串联质谱法快速检测水产品中11种青霉素残留
其他题名	Rapid and Simultaneous Determination of 11 Penicillin Residues in Fishery Products by Pass-through SPE Purification and Liquid Chromatography - Tandem Mass Spectrometry
	郭萌萌 1; 李兆新 1; 王智 2; 潘明轩 1; 吴海燕 1; 邢丽红 1; 孙晓杰 1
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2017
卷号	36 期号:3 页码:337-342
中文摘要	采用通过式固相萃取净化策略去除样品基质中的脂肪和磷脂等杂质干扰,结合液相色谱- 串联质谱检测,建立了水产品中11种青霉素残留的同时快速分析方法。样品经80% 乙腈水溶液提取,Oasis PRiME HLB 通过式固相萃取柱净化,C_(18)色谱柱分离,0. 05% 甲酸乙腈溶液和0. 05% 甲酸水溶液梯度洗脱,多反应监测正离子模式扫描,内标法定量。11种目标物在相应浓度范围内线性关系良好,相关系数不低于0. 99,检出限为0. 30 ~ 1. 5 mug /kg。基质加标回收率为85. 5%~ 110%,相对标准偏差(RSD) 为5. 9%~ 14. 3%。该方法前处理操作简便,灵敏度和准确度高,可实现水产品中多种青霉素药物残留的同时快速测定。
英文摘要	A method was developed for the rapid and simultaneous determination of 11 penicillins in fishery products by pass - through SPE purification with liquid chromatography tandem mass spectrometry( LC - MS /MS). The samples were extracted with acetonitrile - water(80 ∶ 20,by volume), and then cleaned up with an Oasis PRiME HLB pass-through SPE column. The separation of 11 penicillins were performed on a Kinetex XB - C_(18)(2. 1 mm * 100 mm,2. 6 mum) column by using a mixture of 0. 05% formic acid and acetonitrile containing 0. 05% formic acid as mobile phase. Qualitative and quantitative analyses of the analyte were carried out under the multiple reaction monitoring (MRM) mode with positive electrospray ionization. And the isotope internal standards were employed for the quantification. The calibration curves were linear well in corresponding concentration ranges, with correlation coefficient over 0. 99. The detection limits ranged from 0. 30 mug /kg to 1. 5 mug /kg. The average spiked recoveries for 11 penicillins were between 85. 5%and 110% with relative standard deviations(RSDs) of 5. 9% - 14. 3%. The proposed method is accurate,sensitive and high-efficient, and is practical for the determination of penicillins in fishery products.
中文关键词	青霉素 ; 通过式固相萃取 ; 液相色谱- 串联质谱(LC - MS /MS) ; 水产品
英文关键词	penicillins pass-through solid-phase extraction liquid chromatography tandem mass spectrometry(LC - MS /MS) fishery products
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:5946988
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235855
作者单位	1.中国水产科学研究院黄海水产研究所, 农业部水产品质量安全检测与评价重点实验室, 青岛, 山东 266071, 中国; 2.青岛市产品质量监督检验研究院青岛市产品质量检验技术研究所, 青岛, 山东 266061, 中国
推荐引用方式 GB/T 7714	郭萌萌,李兆新,王智,等. 通过式固相萃取净化/液相色谱- 串联质谱法快速检测水产品中11种青霉素残留[J],2017,36(3):337-342.
APA	郭萌萌.,李兆新.,王智.,潘明轩.,吴海燕.,...&孙晓杰.(2017).通过式固相萃取净化/液相色谱- 串联质谱法快速检测水产品中11种青霉素残留.分析测试学报,36(3),337-342.
MLA	郭萌萌,et al."通过式固相萃取净化/液相色谱- 串联质谱法快速检测水产品中11种青霉素残留".分析测试学报 36.3(2017):337-342.