干旱区生态环境知识资源中心(Arid): 羊草根、叶在干旱和盐胁迫下的生理反应

Arid

	羊草根、叶在干旱和盐胁迫下的生理反应
其他题名	Physiological response of leaves and roots of Leymus chinensis under drought and salt stress
	李久道 1; 金华 2; 朴世领 1; 邹吉祥 2; 郭鹏 2; 李琳琳 2; 王智 3
来源期刊	草业科学
ISSN	1001-0629
出版年	2017
卷号	34 期号:8 页码:1705-1710
中文摘要	我国北方干旱气候和盐渍化土地严重限制了羊草(Leymus chinensis)生长,为探究羊草在逆境下根、叶的联系与区别,为提高羊草耐盐性和耐旱性提供理论依据,本研究通过设置不同浓度PEG(20% 、30% 、40% 、50%)溶液和NaCl溶液(100、200、300、400mmol·L~(-1)),模拟不同程度干旱和盐生境,测定干旱胁迫和盐胁迫下羊草根、叶抗氧化酶系统(APX、CAT、POD)活性和可溶性蛋白含量的变化,对比羊草根、叶在胁迫下的生理反应。结果显示,轻度盐胁迫下(100mmol·L~(-1)),羊草主要通过叶片的APX消除活性氧自由基,渗透调节部位主要在叶片中;中度盐胁迫(200、300 mmol·L~(-1))下,根部、叶部分别主要通过提高POD和CAT活性来消除活性氧;胁迫严重时(400mmol·L~(-1)),根部APX显著升高,叶片则通过提高POD和CAT活性共同抵抗活性氧的危害,渗透调节也会从叶片转为根、叶共同作用。干旱胁迫初期(20%),羊草主要依靠根、叶中的APX和叶中的CAT来消除活性氧,随着胁迫加重,根中的APX和叶中的CAT活性提高,共同抵抗活性氧危害,根、叶中POD活性一直呈下降状态,对抵抗活性氧自由基伤害作用不大,渗透调节部位主要在叶片中。
英文摘要	The growth of Leymus chinensis was severely restricted by the arid climate and salinized land in north of China.This test contrasts physiological responses of roots and leaves of L.chinensis under stress,to identify the effects on,and differences between,roots and leaves of L.chinensis by simulating different drought and salt conditions.The benefit of this study is to provide a theoretical basis for enhanced salt tolerance and drought tolerance in L.chinensis.Drought stress and salt stress were respectively simulated by different concentrations of PEG solution and NaCl solution.We discuss the activities of antioxidant enzymes(APX,CAT,and POD) and diversification of soluble protein content in the roots and leaves of L.chinensis.Result:Under mild salt stress(100mmol·L~(-1)),L.chinensis mainly eliminated the ROS through APX of the leaves,and the site of osmotic regulation is mainly in the leaves;under moderate salt stress(200,300mmol·L~(-1)),the roots and leaves eliminated ROS by increasing activities of POD and CAT,and the roots mainly improved activity of POD,and the leaves mainly enhanced activity of CAT;under severe stress(400mmol·L~(-1)),APX of the roots was significantly increased,while the leaves were able to resist damage from ROS by increasing POD and CAT activities,and the location of osmotic regulation also changed from leaves,to roots and leaves.In the early stage of drought stress(20%),L.chinensis mainly relied on APX of roots and leaves and CAT of leaves to eliminate ROS.With the stress increase,activities of APX and CAT in roots were increased to resist the damage from ROS,and the activity of POD in roots and leaves kept decreasing,The location of osmotic adjustment was mainly in leaves.
中文关键词	羊草 ; 根 ; 叶 ; 抗氧化酶活性 ; 可溶性蛋白
英文关键词	Leymus chinensis roots leaves antioxidant enzyme activity soluble protein content
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRONOMY
WOS研究方向	Agriculture
CSCD记录号	CSCD:6069432
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235677
作者单位	1.延边大学农学院, 延吉, 吉林 133000, 中国; 2.大连民族大学环境与资源学院, 大连, 辽宁 116600, 中国; 3.西北农林科技大学林学院, 杨凌, 陕西 712100, 中国
推荐引用方式 GB/T 7714	李久道,金华,朴世领,等. 羊草根、叶在干旱和盐胁迫下的生理反应[J]. 西北农林科技大学,2017,34(8):1705-1710.
APA	李久道.,金华.,朴世领.,邹吉祥.,郭鹏.,...&王智.(2017).羊草根、叶在干旱和盐胁迫下的生理反应.草业科学,34(8),1705-1710.
MLA	李久道,et al."羊草根、叶在干旱和盐胁迫下的生理反应".草业科学 34.8(2017):1705-1710.