干旱区生态环境知识资源中心(Arid): 响应面优化产油沙漠链带藻(Desmodesmus sp.XJ842)的培养基组成

Arid

	响应面优化产油沙漠链带藻(Desmodesmus sp.XJ842)的培养基组成
其他题名	Optimization of culture medium composition for an oil-rich desert microalgae (Desmodesmus sp. XJ842) using response surface methodology
	王程惠; 何梅琳; 邹山梅; 王长海; 邓祥元
来源期刊	中国油脂
ISSN	1003-7969
出版年	2016
卷号	41 期号:1 页码:45-50
中文摘要	以一株从新疆荒漠地区筛选出来的沙漠产油微藻为实验对象,经过形态学和分子生物学手段(18s rRNA及ITS测序)鉴定为链带藻属(Desmodesmus sp.),并命名为XJ842。为提高其油脂产率,采用响应面对其培养基组成(碳源、氮源和磷源)进行优化。单因素实验分析结果显示,其最适产油的碳源为Na_2CO_3,最适氮源为NaNO_3。运用Box-Behnken中心组合实验以及Design-Expert分析软件建立了以总脂含量和油脂产率为响应值的响应面方程,得到最适合产油的Na_2CO_3浓度为1.98 mmol/L,NaNO_3浓度为48.00 mmol/L,K_2HPO_4浓度为0.46 mmol/L。在最优培养基条件下,Desmodesmus sp. XJ842总脂含量达到67.73%,油脂产率达到29.27 mg/(L·d)。优化后的总脂含量、油脂产率比优化前的分别提高了2.08倍和2.61倍。优化后Desmodesmus sp. XJ842油中饱和脂肪酸和单不饱和脂肪酸占总脂肪酸的88.41%,且亚麻酸的含量小于12%,符合欧洲生物柴油标准EN14214,具有生产生物柴油的潜力。
英文摘要	An oil-rich desert microalgae isolated from desert regions in Xinjiang province was identified as Desmodesmus sp. by morphology and molecular biology (18s rRNA and ITS sequencing) and named XJ842. Its composition of culture medium (carbon source,nitrogen source and phosphorus source) was optimized to enhance its oil yield. The analysis results of the single factor experiment showed that the optimal carbon source and nitrogen source were Na_2CO_3 and NaNO_3,respectively. With the total oil content and oil yield as response values,the response surface equation was established by Box-Behnken center combination experiment combined with Design-Expert software. And the optimal concentrations of Na_2CO_3,NaNO_3 and K_2HPO_4 were 1.98,48.00 mmol/L and 0.46 mmol/L,respectively. Under the optimal condition of culture medium,the total oil content and oil yield of Desmodesmus sp. XJ842 reached 67.73% and 29.27 mg/(L·d),respectively. After optimization,the total oil content and oil yield respectively increased by 2.08 times and 2.61 times as many as those before optimization. Meanwhile,the contents of saturated fatty acid and monounsaturated fatty acid in Desmodesmus sp. XJ842 oil accounted for 88.41% of the total fatty acid,and the content of linolenic acid was less than 12%, which complied the European Biodiesel Standards (EN14214) and had potential in biodiesel production.
中文关键词	沙漠微藻 ; 链带藻 ; 响应面优化 ; 培养基组成 ; 生物柴油
英文关键词	desert microalgae Desmodesmus sp. response surface optimization culture medium composition biodiesel
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:5616463
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235545
作者单位	南京农业大学资源与环境科学学院, 南京, 江苏 210095, 中国
推荐引用方式 GB/T 7714	王程惠,何梅琳,邹山梅,等. 响应面优化产油沙漠链带藻(Desmodesmus sp.XJ842)的培养基组成[J],2016,41(1):45-50.
APA	王程惠,何梅琳,邹山梅,王长海,&邓祥元.(2016).响应面优化产油沙漠链带藻(Desmodesmus sp.XJ842)的培养基组成.中国油脂,41(1),45-50.
MLA	王程惠,et al."响应面优化产油沙漠链带藻(Desmodesmus sp.XJ842)的培养基组成".中国油脂 41.1(2016):45-50.