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	盐胁迫对白榆无性系抗氧化酶活性及丙二醛的影响
其他题名	Effects of salt stress on activities of antioxidant enzymes and MDA of elm clones
	苏丹; 李红丽; 董智; 张晓晓; 贾淑友
来源期刊	中国水土保持科学
ISSN	1672-3007
出版年	2016
卷号	14 期号:2 页码:9-16
中文摘要	为了促进滨海盐渍荒漠化地区的植被恢复、引种栽培与盐碱土的改良利用,以3个白榆无性系Y65225、Y46和Y51为试验材料,采用盆栽模拟方法,分析白榆无性系在不同浓度(0.0%、0.3%、0.5%、0.7%和0.9%)NaCl处理下抗氧化酶(SOD、POD和CAT)活性、丙二醛(MDA)含量变化及各指标的相关性,并运用隶属函数法,综合评价3个白榆无性系的耐盐性。结果表明:在各盐浓度胁迫下,Y51能够维持较高的抗氧化酶活性,抗氧化能力最强;Y46的抗氧化酶活性下降最大,抗氧化能力最差,Y46的MDA含量增加幅度最大,受到的盐害最大;Y51的MDA含量增加幅度最小,受到的盐害最小。3个白榆无性系的SOD活性与POD活性,呈极显著正相关,CAT活性与SOD活性、POD活性相关性不显著,CAT、POD活性与MDA含量相关性不强,SOD活性与MDA含量呈显著负相关关系,即SOD活性的增加,可显著减少MDA的积累,增强其抗盐性。综合各项指标,Y51抗盐能力最强,Y65225次之,Y46最差。建议在滨海盐碱地区造林时,优先采用Y51无性系。研究结果将为今后白榆无性系在盐碱地的栽培,提供一定的参考。
英文摘要	[Background] The coastal saline soil is formed by the seawater immersion in the river sediments, with salting and salt characteristics of a typical modern seawater effects. The typical characteristic of coastal saline-alkali soil is that the content of NaCl and the groundwater level are high. Chlorides, sulfates and salt content in the soil directly affect the survival of the germination of seeds, limiting the growth and yield of plants, which has become the most direct factor of vegetation restoration in coastal saline-alkali soil area. Ulmus pumila L. is the most widely distributed and important fast-growing hardwood timber species that has solid tolerance to drought, cold, salt and wind. It is the most important timber species in vast north plains, saline-alkali areas and desert to construct useful afforestation, shelter forest and greenings. [Methods] In order to cultivate, modify and utilize salinized soil of coastal saline area, 3 elm clones (Y65225, Y46 and Y51) were chosen as test materials, watering with 5 salt concentrations of 0.0%, 0.3%, 0.5%, 0.7%, and 0.9% by pot-cultivated experiments in the greenhouse. The activities of dismutase (SOD), catalase (CAT), peroxidase (POD) and mass content of malondialdehyde (MDA) were determined in this study. The change of antioxidant enzymes activity and mass content of MDA and the correlation of the indexes were analyzed. In addition, salt resistance of the 3 clones was comprehensively evaluated by subordination function method. [Results] Y51 sustained high antioxidant enzyme activity under different salt concentrations, meaning it had the strongest antioxidant capacity. While as the antioxidant capacity of Y46 was the worst, because its antioxidant enzyme activity decreased in greatest. MDA was an important indicator of injured cell membrane, which shows that the seedlings are efficiently influenced by the membrane lipid peroxidation. The activities of MDA increased with the salt stress increasing, the greatest increase was from Y46, while the smallest from Y51 and the salt injury to Y51 was the least. The correlation analysis of the measurement indicators for 3 elm clones showed that the correlation of SOD activity and POD activity were significant; besides the CAT activity presented no significant correlation with the above two indicators. The activities of CAT and POD had little significance with the mass content of MDA. Moreover, the activity of SOD showed certain negative correlation with the mass content of MDA, i.e., the increase of SOD significantly reduced the accumulation of MDA mass content, and therefore enhanced the salt resistance of the elm clones. [Conclusions] Ultimately, through overall assessment, salt resistance of Y51 was the highest, less for Y65225 and the lowest for Y46, indicating that they belonged to the middling salt-tolerant elm clones. We recommend that Y51 is the preferred clone in afforestation in the coastal areas. The results of this paper may also provide a certain theoretical significance for cultivating elm clones in the future.
中文关键词	白榆无性系 ; 抗氧化酶 ; 丙二醛 ; 耐盐性 ; 滨海盐碱地
英文关键词	elm clones antioxidant enzyme MDA salt resistance coastal saline area
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FORESTRY
WOS研究方向	Forestry
CSCD记录号	CSCD:5703600
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235500
作者单位	山东农业大学林学院, 山东省土壤侵蚀与生态修复重点实验室, 泰安, 山东 271018, 中国
推荐引用方式 GB/T 7714	苏丹,李红丽,董智,等. 盐胁迫对白榆无性系抗氧化酶活性及丙二醛的影响[J],2016,14(2):9-16.
APA	苏丹,李红丽,董智,张晓晓,&贾淑友.(2016).盐胁迫对白榆无性系抗氧化酶活性及丙二醛的影响.中国水土保持科学,14(2),9-16.
MLA	苏丹,et al."盐胁迫对白榆无性系抗氧化酶活性及丙二醛的影响".中国水土保持科学 14.2(2016):9-16.