干旱区生态环境知识资源中心(Arid): 新疆鼠尾草二萜醌类成分含量测定及抗血小板聚集作用

Arid

	新疆鼠尾草二萜醌类成分含量测定及抗血小板聚集作用
其他题名	Determination of Diterpenoid Quinone in Salvia deserta Roots and Its Inhibition for Platelet Aggregation
	王新玲; 王小青; 胡君萍; 王晓梅; 热娜·卡斯木; 崔智慧
来源期刊	中国实验方剂学杂志
ISSN	1005-9903
出版年	2016
卷号	22 期号:19 页码:48-52
中文摘要	目的:研究新疆鼠尾草中荷茗草醌和7-氧-乙酰荷茗草醌抗血小板聚集作用,并建立同时测定其中荷茗草醌,7-氧-乙酰荷茗草醌和6,7-去氢罗列酮3个主要有效成分含量的方法。方法:应用比浊法测定荷茗草醌和7-氧-乙酰荷茗草醌体外抗血小板聚集作用;采用HPLC同时测定荷茗草醌,7-氧-乙酰荷茗草醌和6,7-去氢罗列酮的含量。结果:荷茗草醌和7-氧-乙酰荷茗草醌高、中剂量组抑制凝血酶诱导的血小板聚集作用与生理盐水组比较均有显著性差异,且与质量浓度呈明显的剂量依赖性;荷茗草醌,7-氧-乙酰荷茗草醌和6,7-去氢罗列酮进样量分别在0.31~4.56 mug(r=0.999 6),0.01~0.24 mug(r=0.999 4),0.4~6.0 mug(r=0.999 5)与峰面积呈良好的线性关系;平均加样回收率分别为100.3%(RSD 2.0%),100.6%(RSD 3.1%),102.5(RSD 1.6%)。结论:新疆鼠尾草中荷茗草醌和7-氧-乙酰荷茗草醌表现出显著抑制血小板聚集作用;建立的HPLC含量测定方法简便快速,结果准确可靠、重复性好,可用于新疆鼠尾草药材的质量控制与评价。
英文摘要	Objective:To evaluate the anti-platelet aggregation effect of horminone and 7-O-acetylhorminone in roots of Salvia deserta, and to establish a method for simultaneous determination of three effective constituents(horminone, 7-O-acetylhorminone and 6,7-dehydroroyleanone) in S. deserta roots. Method: In vitro anti-platelet aggregation of horminone and 7-O-acetylhorminone was determined by turbidimetry. The contents of horminone, 7-O-acetylhorminone and 6,7-dehydroroyleanone were simultaneously determined by HPLC. Result: As compared with the normal saline group, horminone and 7-O-acetylhorminone of the high and middle dose groups could effectively inhibit platelet aggregation induced by thrombin in a clear dose-dependent manner. The horminone, 7-O-acetylhorminone and 6,7-dehydroroyleanone showed good linear relationship with the peak area within 0.31-4.56 mug(r=0.999 6), 0.01-0.24 mug(r=0.999 4) and 0.4-6.0 mug(r=0.999 5); their average recoveries were 100.3%(RSD 2.0%), 100.6%(RSD 3.1%), and 102.5%(RSD 1.6%) respectively. Conclusion: Horminone and 7-O-acetylhorminone in roots of S. deserta showed better anti-platelet aggregation effect. The method of HPLC is simple, accurate and repeatable foe content determination, and can be used for quality control and evaluation of S. deserta.
中文关键词	新疆鼠尾草 ; 高效液相色谱法 ; 荷茗草醌 ; 6,7-去氢罗列酮
英文关键词	Salvia deserta HPLC horminone 6,7-dehydroroyleanone
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:5810965
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235490
作者单位	新疆医科大学药学院, 乌鲁木齐, 新疆 830011, 中国
推荐引用方式 GB/T 7714	王新玲,王小青,胡君萍,等. 新疆鼠尾草二萜醌类成分含量测定及抗血小板聚集作用[J],2016,22(19):48-52.
APA	王新玲,王小青,胡君萍,王晓梅,热娜·卡斯木,&崔智慧.(2016).新疆鼠尾草二萜醌类成分含量测定及抗血小板聚集作用.中国实验方剂学杂志,22(19),48-52.
MLA	王新玲,et al."新疆鼠尾草二萜醌类成分含量测定及抗血小板聚集作用".中国实验方剂学杂志 22.19(2016):48-52.