干旱区生态环境知识资源中心(Arid): 羽毛针禾(Stipagrostis pennata)根鞘中可培养细菌多样性

Arid

	羽毛针禾(Stipagrostis pennata)根鞘中可培养细菌多样性
其他题名	Culture-dependent Bacteria Diversity in Rhizosheath of Stipagrostis pennata
	吴楠 1; 史应武 2; 朱秉坚 1; 张元明1
来源期刊	中国沙漠
ISSN	1000-694X
出版年	2016
卷号	36 期号:3 页码:718-725
中文摘要	羽毛针禾(Stipagrostis pennata)是古尔班通古特沙漠流动沙丘的先锋植物。野生羽毛针禾能够形成特殊的根鞘结构。作为浅根系植物对高温、缺水恶劣环境的特殊应答结构,根鞘对羽毛针禾忍耐干旱胁迫尤为重要。土壤微生物是影响根鞘形成和稳固程度的重要因素,基于前期对羽毛针禾根鞘微生物数量分布特征的分析结果,结合选择性培养基分离纯化、16SrDNA序列测序、Biolog-Eco技术更进一步地分析了可培养细菌多样性特征。结果表明:(1)基于Ribosomal Database Project(RDP)classifier分析,分离、纯化的17株菌可初步归为3门5纲5目8科8属,未见新属。其中,芽孢杆菌属(Bacillus)占绝对优势(23.53%)。(2)EzTaxon-e数据库中对各菌株序列进行进一步的blast同源性分析表明,17株菌可进一步归为13个OTUs,未见疑似新种(同源性均>97%),其中芽孢杆菌属OTU比例仍然最高(23.08%)。(3)根鞘中的土壤细菌群落代谢活性、土壤细菌群落多样性指数(Shannon指数及McIntosh指数)、可培养细菌对糖类、氨基酸类等各类碳源的利用率均显著高于外围土壤。独特的微生物类群是影响根鞘形成的重要外部因素,其与根系分泌物的互动关系值得深入研究。
英文摘要	Stipagrostis pennata is a typical pioneer psammophyte species distribute in deserts of the Junggar Basin in Northern Xinjiang.This psammophyte is resistant to high temperatures,drought,and sandstorms. The presence of rhizosheath makes its ecological function stronger.Rhizosheaths are structures composed of mucilage secreted from plants and adherent soil microbes that form a hard sand cylinder around the root.Our previous study showed that rhizosheath microhabitats were favorable environment for the growth and development of microorganisms.This study focused on investigating the special microbial diversity in the rhizosheath microhabitats.The results showed that:(1)Using three different medium,17 strains were isolated from rhizosheath microhabitats.Phylogenetic analysis of partia1 16S rDNA sequences showed that these strains clustered into 3 phyla,5 classes,5 orders,8 families and 8 genera via Ribosomal Database Project (RDP)classifier.The dominant genera were Bacillus (23.53%).(2)Further classification of these strains was achieved via Basic Local Alignment Search Tool(BLAST)Homology analysis of the partial 16S rDNA against the EzTaxon-e database.BLAST results yielded13 Operational Taxonomic Units(OTUs)and no novel species whose sequence similarity was lower than 97% compare to the species in the EzTaxon-e database. Most of these OTUs belonged to the genus Bacillus(23.08%).(3)Biological Eco technology showed that both the microbial metabolic activity and diversity were higher in the rhizosheaths than that of surrounding soil.It implied that bacteria in the rhizosheaths could well use the carbon substrates.To further discuss the relationship between the exudation from the plant and microbe,we will further investigate not only the influence of root exudation on the growth of dominant species of the microbe in the rhizosheaths,but also their soil binding properties,their mix-effect to the character of rhizosheaths.
中文关键词	RDP在线归类 ; EzTaxon-e数据库 ; Shannon-Wiener多样性指数 ; Biolog法
英文关键词	ribosomal database project(RDP)classifier EzTaxon-e database Shannon-Wiener diversity index biolog-eco technology
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:5743633
来源机构	中国科学院新疆生态与地理研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235417
作者单位	1.中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐, 新疆 830011, 中国; 2.新疆农业科学院微生物应用研究所特殊环境微生物实验室, 乌鲁木齐, 新疆 830091, 中国
推荐引用方式 GB/T 7714	吴楠,史应武,朱秉坚,等. 羽毛针禾(Stipagrostis pennata)根鞘中可培养细菌多样性[J]. 中国科学院新疆生态与地理研究所,2016,36(3):718-725.
APA	吴楠,史应武,朱秉坚,&张元明.(2016).羽毛针禾(Stipagrostis pennata)根鞘中可培养细菌多样性.中国沙漠,36(3),718-725.
MLA	吴楠,et al."羽毛针禾(Stipagrostis pennata)根鞘中可培养细菌多样性".中国沙漠 36.3(2016):718-725.