干旱区生态环境知识资源中心(Arid): 超高效液相-串联质谱法同时测定生鲜乳中45种兽药残留量

Arid

	超高效液相-串联质谱法同时测定生鲜乳中45种兽药残留量
其他题名	Simultaneous determination of 45 kinds of veterinary drug residues in fresh milk by UPLC-MS-MS
	李琴 1; 陶大利 1; 周红 2; 姜金斗 1
来源期刊	中国乳品工业
ISSN	1001-2230
出版年	2016
卷号	44 期号:1 页码:44-47
中文摘要	建立了采用Oasis PriME HLB固相萃取柱净化-超高效液相色谱-串联质谱法同时测定生鲜乳中45种兽药残留含量的方法。该法为样品经质量分数为0.2%甲酸乙腈沉淀蛋白后,通过Oasis PriME HLB固相萃取柱一次性净化除去磷脂及非极性干扰物;用Waters HSS T3色谱柱,流动相为0.1%甲酸水(质量分数10%甲醇)-乙腈(含10%甲醇)梯度洗脱,电喷雾-正离子多反应监测模式(除氯霉素),内标法定量。该方法在质量浓度为1~50ng/mL范围内线性良好,45种兽残相关系数r均大于0.991。经添加1,5,10 mug/kg三个质量分数水平的加标样品的测定,回收率在62.1%~118%之间,RSD%在1.5~9.4之间,方法的准确性和重复性良好。
英文摘要	a method for the simultaneous determination of 45 kinds of veterinary drug residues in fresh milk by PriME HLB Oasis solid phase extraction column purification- ultra high performance liquid chromatography tandem mass spectrometry was established. The method for the sample with 0.2% formic acid acetonitrile protein precipitation, the oasis PriME HLB solid-phase extraction column disposablecleaning to remove phospholipids and non polarity disruptors; waters HSS T3 column. The mobile phase was 0.1% formic acid in water (containing 10% methanol) -acetonitrile (containing 10% methanol gradient elution and electrospray ionization in positive ion multiple reaction monitoring mode (except chloramphenicol), internal standard quantitative method. The method was linear in the range of 1~50 ng/mL, and the correlation coefficient between the 45 species and the residual correlation coefficient r was more than 0.991. With the addition of 1 g/kg,5 g/kg, 10 g/kg three concentration levels, the recovery rate was between 118% and 62.1%, and the accuracy and repeatability of the method was between 9.4 and 1.5.
中文关键词	生鲜乳 ; HLB固相柱净化 ; 超高效液相-串联质谱 ; 兽药残留
英文关键词	PriME fresh milk HLB, PriME solid phase column UHPLC-MS-MS veterinary drug residues
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:5626099
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235376
作者单位	1.农业部乳品质量监督检验测试中心(哈尔滨), 哈尔滨, 黑龙江 150090, 中国; 2.国家乳制品质量监督检验中心, 哈尔滨, 黑龙江 150028, 中国
推荐引用方式 GB/T 7714	李琴,陶大利,周红,等. 超高效液相-串联质谱法同时测定生鲜乳中45种兽药残留量[J],2016,44(1):44-47.
APA	李琴,陶大利,周红,&姜金斗.(2016).超高效液相-串联质谱法同时测定生鲜乳中45种兽药残留量.中国乳品工业,44(1),44-47.
MLA	李琴,et al."超高效液相-串联质谱法同时测定生鲜乳中45种兽药残留量".中国乳品工业 44.1(2016):44-47.