干旱区生态环境知识资源中心(Arid): 超高效液相色谱-质谱法分析人全血、肝脏中维库溴铵

Arid

	超高效液相色谱-质谱法分析人全血、肝脏中维库溴铵
其他题名	Determination of vecuronium bromide in whole blood and blank liver with UPLC-MS/MS
	赵森 1; 王炯 2; 于忠山 2; 谭肖阳 3
来源期刊	药物分析杂志
ISSN	0254-1793
出版年	2016
卷号	36 期号:11 页码:1918-1924
中文摘要	目的:建立超高效液相色谱-质谱法(UPLC-MS/MS法)测定人血液、肝脏中维库溴铵。方法:取空白血、空白肝(经匀浆)加入一定量维库溴铵标准溶液,加入pH 8氨水稀释后涡旋离心,取上清液过混合型弱阳离子交换柱(WCX)进行固相萃取提取纯化。提取液采用UPLC-MS/MS检测。色谱分离采用UPLC? BEH HILIC色谱柱。流动相体系:A相为乙腈,B相为5 mmol · L~(-1)甲酸铵水溶液(甲酸调pH 3.5左右),梯度淋洗;质谱检测采用正离子扫描,多反应离子监测模式(MRM)。检测结果以维库溴铵母离子m/z 557.526和子离子m/z 100.103、356.346进行定性、定量。结果:空白血、空白肝加标回收率在74%~120%,检出限为0.43 ng·mL~(-1)。结论:本文通过初步探讨维库溴铵大鼠体内分布,得知维库溴铵在大鼠体内的分布变化是受多种复杂因素影响的动态变化过程,并且死后随提取检材部位的不同测出药物浓度存在较大差异。真实案例中也证实了维库溴铵体内分布这一特点。本文可以为相关中毒案(事)件的检验提供方法,并且满足检验要求。
英文摘要	Objective: To establish a UPLC-MS/MS method for the quantitative determination and confirmation of vecuronium bromide in whole blood and liver. Methods: The whole blood and blank liver were added with vecuronium bromide and then diluted by ammonia water, followed by vortexing and centrifuging. The supernatant liquid was cleaned-up by solid phase extraction technique using Oasis WCX. Extracts were analyzed by UPLC-MS/MS in positive ionization. An acquity UPLC? BEH HILIC column was used with gradient elution of acetonitrile and 5 mmol · L~(-1) ammonium formate buffer(pH=3.5) as the mobile phase,and the signals were detected by ESI source in MRM mode operated in positive modes. The parent ion for vecuronium bromide is m/z 557.526. Two daughter ions were monitored at m/z 356.346 and 100.103 for quantification and confirmation. Results: The average recovery of vecuronium bromide from the whole blood samples and the blank liver was 74%-120%. The detection limit was 0.43 ng · mL~(-1). Conclusion: From the preliminary discussion on distribution of vecuronium bromide in rats, it was known that the distribution variation of vecuronium bromide in rats was a dynamic variation process influenced by multiple complex factors and there was a postmortem maldistribution of vecuronium bromide in poisoning death rats. The distribution of VEC in poisoning death rats was confirmed by the real case. This paper could provide test method for related poisoning cases or events, and the method meets the test requirements.
中文关键词	单季铵盐类化合物 ; 肌肉松弛药 ; 全麻辅助用药 ; 维库溴铵 ; 血液 ; 肝 ; 基质效应 ; 动态分布 ; 药物中毒检测 ; 用药安全 ; 超高效液相色谱-质谱法
英文关键词	quaternary ammonium compounds muscle relaxant auxiliary medication of general anesthesia vecuronium bromide whole blood liver matrix effect dynamic distribution drug toxicosis test drug safety UPLC-MS/MS
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PHARMACOLOGY PHARMACY
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:5854183
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235228
作者单位	1.浙江警察学院, 杭州, 浙江 310053, 中国; 2.公安部物证鉴定中心, 北京 100038, 中国; 3.山东淄博市公安司法鉴定中心, 淄博, 山东 255000, 中国
推荐引用方式 GB/T 7714	赵森,王炯,于忠山,等. 超高效液相色谱-质谱法分析人全血、肝脏中维库溴铵[J],2016,36(11):1918-1924.
APA	赵森,王炯,于忠山,&谭肖阳.(2016).超高效液相色谱-质谱法分析人全血、肝脏中维库溴铵.药物分析杂志,36(11),1918-1924.
MLA	赵森,et al."超高效液相色谱-质谱法分析人全血、肝脏中维库溴铵".药物分析杂志 36.11(2016):1918-1924.