干旱区生态环境知识资源中心(Arid): 蒙古沙冬青伴生植物AM真菌多样性

Arid

	蒙古沙冬青伴生植物AM真菌多样性
其他题名	Diversity of Arbuscular Mycorrhiza Fungi near to the Associated Plants of Ammopiptanthus mongolicus
	胡从从 1; 郭清华 1; 贺学礼 1; 赵丽莉 1; 李英鹏 1; 曹翠兰 2
来源期刊	西北农业学报
ISSN	1004-1389
出版年	2016
卷号	25 期号:6 页码:921-932
中文摘要	为探究蒙古沙冬青(Ammopiptanthus mongolicus)伴生植物丛枝菌根(AM)真菌遗传多样性特征,于2013年7月随机取样采集内蒙古乌海、磴口和乌拉特后旗3个样地霸王(Zygophyllum xanthoxylum)和白刺(Nitraria tangutorum)根围土壤,利用变性梯度凝胶电泳(DGGE)技术对AM真菌群落结构、生态分布及与土壤因子的相关性进行研究。结果表明:(1)共分离鉴定球囊霉属(Glomus)、管柄囊霉属(Funneliformis)和根内球囊霉属(Rhizophagus)3属AM真菌,其中,Glomus为所有样地和土层共有优势属,G.coronatum(AJ276086.2)和G.sp.(KC558550.1)为霸王根围优势菌种,Uncultured Funneliformis(HG004455.1)和Uncultured Rhizophagus(KC797139.1)为白刺根围优势菌种;G.coronatum(FR773145.1)是乌海特有菌种,G.sp.(AJ315521.1),Uncultured Rhizophagus(KF386282.1)和Uncultured Glomus(HG972422.1)为磴口特有菌种。(2)不同宿主、样地、土层AM真菌具有不同的DGGE图谱特征,丰度和多样性指数呈现蒙古沙冬青>霸王>白刺;同一样地,0~30 cm土层高于30~50 cm土层;同一土层,乌海、磴口均高于乌拉特后旗样地,其中乌海20~30 cm土层AM真菌丰度和多样性指数最高,分别为16和2.06。(3)土壤速效磷与AM真菌多样性显著正相关,碱解氮、碱性磷酸酶与AM真菌群落多样性显著负相关。可见,AM真菌能与宿主植物形成良好共生关系,具有丰富的遗传多样性;宿主植物、环境因子对AM真菌群落多样性有重要影响。
英文摘要	Ammopiptanthus mongolicus is an excellent sand-fixation and endangered desert ecosystem of leguminous plants.Zygophyllum xanthoxylum and Nitraria tangutorum are associated plants of A.mongolicus that can not only adapt to the desert environment but also repair the ecological system.In order to explore the community composition and genetic diversity of AM fungi in the rhizosphere soil of Z.xanthoxylum and N.tangutorum by PCR-DGGE,in July 2013,the soil samples were collected from three different sites in Wuhai,Dengkou and Wulate of Inner Mongolia,China.Results showed that:(1) AM fungi have abundant genetic diversity.Three genera,including Glomus,Funneliformis and Rhizophagus,were isolated and identified in the rhizosphere soil of Z.xanthoxylum and N.tangutorum.Glomus was dominant genus of all the sample area and soil layer.The superiority strains of Z.xanthoxylum were G.coronatum(AJ276086.2)and G. sp.(KC558550.1).Uncultured Funneliformis(HG004455.1)and Uncultured Rhizophagus(KC797139.1)were the dominant species of N.tangutorum.G.coronatum(FR773145.1)was a special species in Wuhai.G.sp.(AJ315521.1).Uncultured Rhizophagus(KF386282.1)and Uncultured Glomus(HG972422.1)were unique species in Dengkou.(2) The fingerprint characteristics of DGGE,richness,diversity index and dominance of AM fungi varied with host plants,sampling sites and soil depth.Richness and diversity index of AM fungi were higher under A.mongolicus than under the associated plants and in 0-30 cm soil layer than that in 30-50 cm soil layer.For the same soil layer,the richness and diversity index of AM fungi were sequenced in a descending order in Wuhai,Dengkou,and Wulate.The highest richness and diversity index at 20-30 cm soil layer in Wuhai,reaching 16 and 2.06,respectively.(3) Soil available phosphorus had a positive correlation with AM fungi diversity.Available nitrogen,alkaline phosphatase had significantly negative correlation with AM fungi community diversity.The results reflected AM fungi can form good symbiosis with the host plant and have abundant genetic diversity.The host plants and environmental factors significantly influenced the community composition and ecological distribution of AM fungi.
中文关键词	蒙古沙冬青 ; 伴生植物 ; AM真菌 ; 遗传多样性
英文关键词	PCR-DGGE Ammopiptanthus mongolicus Associated plants AM fungi PCR- DGGE Genetic diversity
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MICROBIOLOGY
WOS研究方向	Microbiology
CSCD记录号	CSCD:5737229
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235147
作者单位	1.河北大学生命科学学院, 保定, 河北 071002, 中国; 2.西北农林科技大学生命科学学院, 杨凌, 陕西 712100, 中国
推荐引用方式 GB/T 7714	胡从从,郭清华,贺学礼,等. 蒙古沙冬青伴生植物AM真菌多样性[J]. 西北农林科技大学,2016,25(6):921-932.
APA	胡从从,郭清华,贺学礼,赵丽莉,李英鹏,&曹翠兰.(2016).蒙古沙冬青伴生植物AM真菌多样性.西北农业学报,25(6),921-932.
MLA	胡从从,et al."蒙古沙冬青伴生植物AM真菌多样性".西北农业学报 25.6(2016):921-932.