干旱区生态环境知识资源中心(Arid): 白皮锦鸡儿种子内生细菌的分布与分离鉴定

Arid

	白皮锦鸡儿种子内生细菌的分布与分离鉴定
其他题名	Isolation and characterization of endophytic colonizing bacteria from the seed of Caragana leucophloea Pojark
	时晓寒; 程聪; 李玉倩; 安登第
来源期刊	微生物学通报
ISSN	0253-2654
出版年	2016
卷号	43 期号:1 页码:164-171
中文摘要	【目的】了解对荒漠环境有良好适应性的白皮锦鸡儿种子可培养内生细菌的多样性。【方法】采用种子表面灭菌、种皮与种仁分离、可培养细菌分离纯化、对pH及盐的耐受性、16S rRNA基因序列扩增和系统发育分析进行系统研究。【结果】从白皮锦鸡儿成熟种子种皮和种仁分别分离纯化到6株和26株内生细菌,其中分离自种皮的菌株最适pH均在9.0以上,且有3株最高可耐受pH 14.0,有5株可耐受10%的NaCl;分离自种仁的菌株pH耐受范围明显低于种皮,但有12株对NaCl的耐受性可达10%。16S rRNA基因序列分析显示,30株与芽孢杆菌属(Bacillus sp.)接近,相似率95%-99%,其中13株与枯草芽孢杆菌(Bacillus subtilis)的相似率为96%-99%, 11株与地衣芽孢杆菌(Bacillus licheniformis)相似率为95%-99%;1株与单孢菌属(Sphingomonas sp.)相似性为99%,1株与大肠杆菌属(Escherichia sp.)相似性为98%。【结论】白皮锦鸡儿种仁内生菌数量和种类均高于种皮,体现出其可培养内生菌在空间分布的分异性及种类的多样性,且推测其种皮内生菌对盐碱的高度耐受性应与其环境适应性相关。
英文摘要	[Objective] This paper aimed to estimate the diversity and characterization of endophytic bacteria isolated from seed of Caragana leucophloea Pojark which good adaptability to desert. [Methods] The methods used in this study include seeds surface sterilization, seed coat and kernel separation, endophytic bacteria isolation, tolerance to pH and NaCl estimating, 16S rRNA gene sequencing and phylogenetic analyzing. [Results] With the thirty-two isolates, six and twenty-six strains were obtained from seed coat and kernel, respectively. The optimal pH for all strains that from seed coat was above pH 9.0 and the maximum was up to 14.0 for three of them, and the tolerance to salt was up to 10% NaCl for five isolates. The tolerance to pH of the strains isolated from kernel was significantly lower than that from seed coat, but there’s twelve strains could tolerant to salt up to 10% NaCl also. According to 16S rRNA gene sequences analysis, thirty strains belong to Bacillus sp. with the similarity between 95%-99%. Among them, thirteen isolates was closed to Bacillus subtilis with similarity 96%-99% and eleven was closed to Bacillus licheniformis with similarity 95%-99%. One strain belong to genus Sphingomonas sp. with the homology of 99%, and one belong to genus Escherichia sp. with the homology of 98%. [Conclusion] The results has showed that the diversity of endophytic bacteria of C. leucophloea’s kernel was higher than that of the coat, indicating their space distribution and species diversity. And we could speculate that the higher tolerance on pH and salt of the bacteria of the seed coat must have some relationship with the host environment adaptability.
中文关键词	内生细菌 ; 空间分布 ; 生物学特性 ; 分子鉴定 ; 白皮锦鸡儿
英文关键词	Endophytic bacteria Space distribution Biological charcteristics Molecular identification Caragana leucophloea Pojark
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRONOMY ; MICROBIOLOGY
WOS研究方向	Agriculture ; Microbiology
CSCD记录号	CSCD:5614813
来源机构	新疆师范大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/235102
作者单位	新疆师范大学生命科学学院, 乌鲁木齐, 新疆 830054, 中国
推荐引用方式 GB/T 7714	时晓寒,程聪,李玉倩,等. 白皮锦鸡儿种子内生细菌的分布与分离鉴定[J]. 新疆师范大学,2016,43(1):164-171.
APA	时晓寒,程聪,李玉倩,&安登第.(2016).白皮锦鸡儿种子内生细菌的分布与分离鉴定.微生物学通报,43(1),164-171.
MLA	时晓寒,et al."白皮锦鸡儿种子内生细菌的分布与分离鉴定".微生物学通报 43.1(2016):164-171.