干旱区生态环境知识资源中心(Arid): 沼液还田对旱地红壤微生物群落代谢与多样性的影响

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	沼液还田对旱地红壤微生物群落代谢与多样性的影响
其他题名	Analysis on metabolic characteristics and functional diversity of soil edaphon communities in upland red soil under biogas slurry application
	郑学博 1; 樊剑波 1; 崔键 1; 徐磊 1; 祝振球 1; 周静 2; 何园球 1
来源期刊	生态学报
ISSN	1000-0933
出版年	2016
卷号	36 期号:18 页码:5865-5875
中文摘要	通过沼液还田定位实验,按照不同沼液全氮还田比例设6个等氮量(N-P_2O_5-K_2O量均为120-90-135 kg/hm~2(对照除外))处理:对照(不施肥,CK)、100%化学氮(NPK)、15%沼液氮+85%化学氮(BS15)、30%沼液氮+70%化学氮(BS30)、45%沼液氮+ 55%化学氮(BS45)和100%沼液氮(BS100),运用Biolog-ECO技术分析0-20cm花生收获期土壤微生物群落代谢功能多样性,阐明微生物群落代谢与沼液还田量的相关关系。结果表明:①BS45、BS30处理土壤微生物群落碳源代谢强度(AWCD)显著高于CK和NPK处理;而BS15、BS100处理土壤微生物群落碳源代谢强度(AWCD)与CK和NPK处理则无显著差异;②土壤微生物群落碳源代谢强度(AWCD)、丰富度指数、Shannon指数、Simpson优势度指数均表现为BS45>BS30> NPK > CK >BS100> BS15;③结合主成分分析和聚类分析,表明各处理土壤微生物群落功能多样性分为4组:BS45、BS30处理为一组,微生物群落代谢活性最强,特别是碳水化合物、氨基酸、聚合物和胺类等碳源的代谢能力;NPK、CK、BS100处理为一组,微生物群落代谢活性次之;BS15处理为一组,微生物群落代谢能力最低,其碳水化合物、羧酸、氨基酸、聚合物、酚类和胺类等碳源的代谢能力均为最低。结合主成分分析综合得分,土壤微生物群落代谢和多样性的顺序为BS45>BS30> NPK > CK >BS100>BS15。可见,沼液还田显著影响旱地红壤微生物群落的代谢活性和多样性,沼液不能完全替代化肥,当沼液全氮还田比例在30% 45%时,微生物群落代谢活性最强,有利于土壤质量提高,适于在我国旱地红壤地区推广。
英文摘要	In order to provide a theoretical basis for soil edaphon community improvement and a fertilization system establishment for arid areas in south China, a field experiment was conducted to determine the effects of biogas slurry returning systems on the metabolic characteristics and functional diversity of soil edaphon communities in upland red soil. The experiment contained six treatments: nofertilizer (CK), chemical fertilizer (NPK), 15% biogas slurry N + 85% chemical fertilizer N (BS15), 30% biogas slurry N + 70% chemical fertilizer N (BS30), 45% biogas slurry N + 55% chemical fertilizer N (BS45),and 100% biogas slurry N (BS100). Each treatment included the same amounts of N-P_2O_5-K_2O = 120-90- 135 kg/hm~2(except for CK). Average well color development (AWCD), indexes of microbial functional diversity (Shannon index,Simpson index,richness index) were determined,and principal component analysis (PCA) and clustering analysis were conducted. The results showed that the AWCD value of treatments BS45 and BS30 was significantly higher than that of CK and NPK treatments, respectively. Treatments BS15 and BS100 showed no significant difference to CK and NPK treatments, respectively. The order of AWCD value, Richness index, Shannon index, and Simpson index wereall the same as follow: BS45>BS30> NPK > CK >BS100>BS15. The results of PCA and clustering analysis showed that the functional diversity of soil edaphoncan develop into 4 groups: First were treatments BS45 and BS30 with the highest microbial activity, which promoted microbial activity to metabolize the carbon sources of carbohydrates, amino acids, polymers and amines; Second were treatments NPK and CK with second highest microbial activity; Thirdwas treatment BS100 with the third highest microbial activity, and fourth was treatment BS15 with the lowest microbial activity. Combined with the PCA score, the order of functional diversity of edaphon communities was BS45>BS30> NPK > CK>BS100>BS15. Overall, we conclude that the microbial activity and functional diversity of upland red soil was significantly affected by biogas slurry application, and that the chemical fertilizer was not completely replaced by biogas slurry. The soil microbial activity reached the highest level when the total nitrogen input ratio of biogas slurry to chemical fertilizer rangedfrom 30% to 45%,which could be expanded inupland red soil of south China.
中文关键词	沼液 ; 旱地红壤 ; 微生物代谢特征 ; 群落多样性
英文关键词	Biolog-ECO biogas slurry upland red soil Biolog-ECO metabolic characteristic microbial diversity
语种	中文
国家	中国
收录类别	CSCD
WOS类目	BIOLOGY
WOS研究方向	Life Sciences & Biomedicine - Other Topics
CSCD记录号	CSCD:5806878
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/234811
作者单位	1.中国科学院南京土壤研究所, 南京, 江苏 210008, 中国; 2.中国科学院南京土壤研究所, 国家红壤改良工程技术研究中心, 南京, 江苏 210008, 中国
推荐引用方式 GB/T 7714	郑学博,樊剑波,崔键,等. 沼液还田对旱地红壤微生物群落代谢与多样性的影响[J],2016,36(18):5865-5875.
APA	郑学博.,樊剑波.,崔键.,徐磊.,祝振球.,...&何园球.(2016).沼液还田对旱地红壤微生物群落代谢与多样性的影响.生态学报,36(18),5865-5875.
MLA	郑学博,et al."沼液还田对旱地红壤微生物群落代谢与多样性的影响".生态学报 36.18(2016):5865-5875.