干旱区生态环境知识资源中心(Arid): 磁性石墨烯分散微固相萃取-液相色谱-四极杆串联质谱法测定畜禽肉中9种非甾体抗炎剂

Arid

	磁性石墨烯分散微固相萃取-液相色谱-四极杆串联质谱法测定畜禽肉中9种非甾体抗炎剂
其他题名	Magnetic graphene based dispersive micro-solid-phase extraction coupled with liquid chromatography-tandem quadrupole mass spectrometry for the analysis of nine non-steroidal anti-inflammatory drugs in livestock and poultry meat
	董婵婵 1; 胡艳云 2; 吕亚宁 3; 宋伟 3; 韩芳 3; 郑平 3; 邓宁 1
来源期刊	色谱
ISSN	1000-8713
出版年	2016
卷号	34 期号:9 页码:850-859
中文摘要	以磁性石墨烯纳米复合材料(Fe_3O_4-G)作为吸附剂,建立了磁性分散微固相萃取-液相色谱-四极杆串联质谱(LC-MS/MS)测定畜禽肉样品中9种非甾体抗炎剂(NSAIDs)残留的方法。试样经酸化乙腈均质、冷冻离心除油、乙腈饱和正己烷脱脂后,采用磁性分散微固相萃取净化。对影响萃取效率的因素(萃取时间、样品溶液的pH值和洗脱条件)进行了优化。9种非甾体抗炎剂的检出限(LOD, S/N=3)为0.2~8.2 mug/kg,定量限(LOQ, S/N=10)为0.5~25.4 mug/kg。在添加水平分别为LOQ、2倍LOQ和10倍LOQ时,9种药物的加标回收率为83.3%~104.5%,相对标准偏差为1.2%~6.8%。与Sep-Pak Vac NH_2和Oasis HLB固相萃取柱相比,磁性分散微固相萃取方法富集、分离效果好,负载量大,可重复利用,为畜禽肉中非甾体抗炎剂残的测定提供了一种新的前处理技术。
英文摘要	A novel analytical method was developed for the determination of nine non-steroidal anti-inflammatory drugs (NSAIDs) in meat samples by magnetic graphene nanocomposite (Fe_3O_4-G) based on dispersive micro-solid-phase extraction (mu-SPE) coupled with liquid chromatography-mass spectrometry detection. Samples were homogenized and extracted with acidified acetonitrile. After defatting with the high speed frozen centrifugation, the sample solutions were defatted with n-hexane followed by mu-SPE. Some important parameters influencing the extraction efficiency including the extraction time, the pH value of sample solution and the elution conditions were optimized. The limits of detection (LODs, S/N=3) of the nine NSAIDs ranged from 0.2 to 8.2 mug/kg, and the limits of quantification (LOQs, S/N=10) ranged from 0.5 to 25.4 mug/kg. The average recoveries were 83.3%-104.5% with the relative standard deviations between 1.2% and 6.8% obtained from samples spiked at LOQ, 2LOQ and 10LOQ levels. Compared with Sep-Pak Vac NH_2 and Oasis HLB cartridges, Fe_3O_4-G has excellent purification effect, high loading amounts and good reusability. The results demonstrated that the method provide a novel pretreatment technique for the determination of NSAID residues in meat samples.
中文关键词	磁性分散微固相萃取 ; 液相色谱-四极杆串联质谱 ; 磁性石墨烯纳米复合材料 ; 非甾体类抗炎剂 ; 畜禽肉
英文关键词	dispersive micro-solid-phase extraction liquid chromatography-quadrupole mass spectrometry (LC-MS/MS) magnetic graphene nanocomposite non-steroidal anti-inflamma-tory drugs livestock and poultry meat
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:5790223
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/234696
作者单位	1.合肥工业大学化学与化工学院, 合肥, 安徽 230009, 中国; 2.安徽出入境检验检疫局, 合肥, 安徽 230022, 中国; 3.安徽出入境检验检疫局, 食品安全分析与检测安徽省重点实验室, 合肥, 安徽 230022, 中国
推荐引用方式 GB/T 7714	董婵婵,胡艳云,吕亚宁,等. 磁性石墨烯分散微固相萃取-液相色谱-四极杆串联质谱法测定畜禽肉中9种非甾体抗炎剂[J],2016,34(9):850-859.
APA	董婵婵.,胡艳云.,吕亚宁.,宋伟.,韩芳.,...&邓宁.(2016).磁性石墨烯分散微固相萃取-液相色谱-四极杆串联质谱法测定畜禽肉中9种非甾体抗炎剂.色谱,34(9),850-859.
MLA	董婵婵,et al."磁性石墨烯分散微固相萃取-液相色谱-四极杆串联质谱法测定畜禽肉中9种非甾体抗炎剂".色谱 34.9(2016):850-859.