干旱区生态环境知识资源中心(Arid): 新疆戈壁野生木贼麻黄中麻黄碱和盐酸伪麻黄碱含量的测定

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	新疆戈壁野生木贼麻黄中麻黄碱和盐酸伪麻黄碱含量的测定
其他题名	Determination of Ephedrine and Pseudoephedrine of Wild E. equisetina Produced from Gobi Desert in Xinjiang Region
	木合塔尔·吐尔洪 1; 楚刚辉 1; 木尼热·阿不都克里木 2
来源期刊	南开大学学报. 自然科学版
ISSN	0465-7942
出版年	2016
卷号	49 期号:5 页码:74-78
中文摘要	采用半仿生提取法提取生物碱,以薄层色谱法(TLC)对戈壁野生木贼麻黄的根、茎与果中提取的生物碱进行了签别,用HPLC同时测定新疆和田皮山戈壁野生木贼麻黄中的盐酸麻黄碱与盐酸伪麻黄碱等生物碱的含量,色谱柱为Agilent HC-C 18(4.6 mm*250 mm,5 mum),流动相为甲醇-0.2%磷酸水溶液(体积比10:90),测定波长为210 nm,流速为1.0 mL/mm.结果显示,测定盐酸麻黄碱和盐酸伪麻黄碱分别在0.7-4.2 mug/mL(r= 0.999),0.05-0.3 mug/mL(r=0.999)范围内,线性关系良好,平均加标回收率分别为101.6%(RSD = 0.3%),102.1%(RSD = 0.7%).该法结果准确,重现性好,可用于新疆和田皮山戈壁野生木贼麻黄2种生物碱的含量测定.
英文摘要	To establish a method for determining ephedrine and pseudoephedrine of wild E. equisetina produced from gobi desert in Xinjiang region by HPLC. The ephedrine was extracted by semi-bionic extraction (SBE), and thin-layer chromatography (TLC) was used to identify ephedrine and pseudoephedrine in it, and then their contents were measured by high performance liquid chromatography (HPLC). The column was Agilent HC-C18 column (250 mm*4.6 mm, 5 mum). The mobile phase was methanol and 0.2% phosphoric acid (10:90). Detection wavelength was 210 nm and flow rate was 1.0 ml/min. Ephedrine and pseudoephedrine have good linear relationship in the range of 0.7-4.2 mug/mL (r= 0.999) and 0.05-0.3 mug/ mL (r = 0.999). The average recovery was 101.6% (RSD = 0.3%) and 102.1% (RSD = 0.7%), respectively. The result was accurate, and reproducibility was good. The method can be used for determining of ephedrine and pseudoephedrine of wild E. equisetina produced from gobi desert in Xinjiang region.
中文关键词	野生木贼麻黄 ; 盐酸麻黄碱 ; 盐酸伪麻黄碱 ; 薄层色谱 ; 高效液相色谱法
英文关键词	wild E. equisetinu ephedrine hydrochloride pseudoephedrine hydrochloride HPLC
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:5846779
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/234578
作者单位	1.喀什大学化学与环境科学学院, 新疆特色药食用植物资源化学实验室, 喀什, 新疆 844006, 中国; 2.喀什大学化学与环境科学学院, 喀什, 新疆 844006, 中国
推荐引用方式 GB/T 7714	木合塔尔·吐尔洪,楚刚辉,木尼热·阿不都克里木. 新疆戈壁野生木贼麻黄中麻黄碱和盐酸伪麻黄碱含量的测定[J],2016,49(5):74-78.
APA	木合塔尔·吐尔洪,楚刚辉,&木尼热·阿不都克里木.(2016).新疆戈壁野生木贼麻黄中麻黄碱和盐酸伪麻黄碱含量的测定.南开大学学报. 自然科学版,49(5),74-78.
MLA	木合塔尔·吐尔洪,et al."新疆戈壁野生木贼麻黄中麻黄碱和盐酸伪麻黄碱含量的测定".南开大学学报. 自然科学版 49.5(2016):74-78.