干旱区生态环境知识资源中心(Arid): 基于bchY 的内蒙古2个盐碱湖中不产氧光合细菌多样性的分析

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	基于bchY 的内蒙古2个盐碱湖中不产氧光合细菌多样性的分析
其他题名	Diversity of Anoxygenic Photosynthetic Bacterial and Environment Adaptation Relationship in Dagenao and Zagesitai Haloalkaline Lakes in Desert Hunshandake
	宝力德; 冀锦华; 刘柯澜; 冯福应
来源期刊	华北农学报
ISSN	1000-7091
出版年	2016
卷号	31 期号:3 页码:147-154
中文摘要	为进一步了解和认识APB 及其在盐碱湖中的生态功能及其作用机制,通过构建和分析不产氧光合细菌 bchY 基因克隆文库,揭示其中不产氧光合细菌的多样性、群落结构及其与生存环境的关系。样品采自内蒙古浑善达克沙地盐碱度较高的扎格斯台和达格淖2个湖的水样,提取了样品总DNA。以bchY 作为检测APB 的通用标记,用 PCR 扩增方法构建了其bchY 文库,并对扩增产物进行了测序与系统发育分析。结果表明,达格淖湖和扎各斯台湖均为富营养化盐碱湖,且两湖盐度相差很大。利用bchY 文库中核酸序列对应的氨基酸序列进行分析,两文库覆盖度均达93. 0%以上,代表性较强,共得到108条bchY 编码的氨基酸序列,以cutoff = 0. 03和0. 12分别在种和属水平进行同源性和系统发育分析,在cutoff = 0. 03分为17个OTUs。两湖的多样性指数( Shannon-Weaver 和Simpson)和均匀度指数( Evenness)较接近,其种群多样性程度和均匀程度比较相似,但二者的Chao1都不太高,说明两湖APB 的多样性较低,种群不丰富。达格淖湖的Margalef 指数较高,说明前者物种数目比后者多。两湖的不产氧光合细菌均属Alphaproteobacteria纲,最优势菌群均为Roseibacterium elongatum。同源性及系统发育性分析表明,达格淖湖和扎各斯台湖中不产氧光合细菌的主要类型为AAPB-like 类群、多样性低。
英文摘要	Reports about research on the diversity of Anoxygenic phototrophic bacteria( APB) in haloalkaline lakes are too rarely. This experiment constructed and analyzed Anoxygenic phototrophic bacteria bchY gene library of Dagenao Lake and Zagesitai Lake to reveal the diversity and community structure of APB in two lakes and their relation with living environment for the sake of understanding APB and their ecology function and its mechanism. The water samples were collected from the Dagenao Lake and Zagesitai Lake which have higher salinity in Hunshandake desert in Inner Mongolia,and their total DNA were extracted. Their bchY libraries were constructed by PCR amplification method using bchY as standard marker for the detection of APB, the amplification products were sequenced and the phylogenetic analysis has been carried out. The results showed that Dagenao Lake and Zagesitai Lake were Eutrophical haloalkaline lakes and the salinity of two lakes was obviously different. Analysis of the amino acid sequences encoded by bchY nucleotide sequences in two libraries discovered that the coverages of two lakes were over 93% and strongly representative. 108 amino acid sequences encoded by bchY were obtained from the two libraries totally and these sequences were divided into 17 operational taxonomic units( OTUs) with the cutoff value of 0. 03 and analyzed the similarity and phylotype composition under the genus( 0. 12) and species( 0. 03) lever. The diversity index and evenness index of two lakes were too approaching, therefore the diversity and evenness of species in two lakes were very similar,while their Chao1 values were law, indicated that the diversities of APB in two lakes were relatively low and the species were not too abundant. The Margalef index of Dagenao Lake was more higher,which indicated that the number of species in Dagenao Lake was more than Zagesitai Lake. In both libraries all of APB belonged to Alphaproteo bacteria class,and the predominant OTUs was Roseibacterium elongatum,diversity of APB in two lakes were similar and lower. Analysis of the similarity and phylotype composition revealed that the main type of APB was AAPB-like group,and the diversity of APB was lower. This study provided theoretical foundation and basis for understanding APB and their ecological role and function.
中文关键词	盐碱湖 ; 不产氧光合细菌( APB) ; bchY 基因 ; cutoff 值 ; 系统发育分析 ; 多样性 ; 基因克隆文库
英文关键词	OTUs Haloalkaline lake Anoxygenic phototrophic bacteria( APB) bchY gene Cutoff value OTUs Analysis of phylotype composition Diversity Gene library
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MICROBIOLOGY
WOS研究方向	Microbiology
CSCD记录号	CSCD:5749067
来源机构	内蒙古农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/234485
作者单位	内蒙古农业大学生命科学学院, 呼和浩特, 内蒙古 010018, 中国
推荐引用方式 GB/T 7714	宝力德,冀锦华,刘柯澜,等. 基于bchY 的内蒙古2个盐碱湖中不产氧光合细菌多样性的分析[J]. 内蒙古农业大学,2016,31(3):147-154.
APA	宝力德,冀锦华,刘柯澜,&冯福应.(2016).基于bchY 的内蒙古2个盐碱湖中不产氧光合细菌多样性的分析.华北农学报,31(3),147-154.
MLA	宝力德,et al."基于bchY 的内蒙古2个盐碱湖中不产氧光合细菌多样性的分析".华北农学报 31.3(2016):147-154.