干旱区生态环境知识资源中心(Arid): 羊柴根围AM真菌群落结构和遗传多样性研究

Arid

	羊柴根围AM真菌群落结构和遗传多样性研究
其他题名	Community composition and genetic diversity of arbuscular mycorrhizal fungi in the rhizosphere of clonal plants Hedysarum leave Maxim
	成斌; 胡从从; 赵丽莉; 贺学礼
来源期刊	河北农业大学学报
ISSN	1000-1573
出版年	2016
卷号	39 期号:3 页码:23-30
中文摘要	为了探明受风蚀和水蚀双重影响下内蒙古荒漠地区羊柴(Hedysarum leave Maxim)根围AM真菌群落结构和遗传多样性特征,以内蒙古正蓝旗青格勒图具有代表性的梁地作为采样地点,按海拔高度在梁地选择梁底、梁中和梁顶3个样地,分0~10,10~20,20~30,30~40,40~50cm 5个土层采集羊柴根围土壤样品,通过Nested-PCR-DGGE技术,结合DGGE图谱分析、DNA克隆测序及系统发育分析进行研究。结果表明,不同样地和土层,AM真菌具有不同DGGE指纹图谱特征,AM真菌丰度、优势度及其多样性指数均有差异,其中梁中和梁顶样地0~10cm土层AM真菌丰富度最高,均为20;多样性指数梁中样地0~10cm土层最高,为2.88。 DGGE条带测序和系统发育分析显示,全部序列归为5属15种,分别为Glomus属10种、Rhizophagus属1种、 Funneliformis属2种、Paraglomus属1种、Septoglomus属1种;根据UPGMA算法绘制15个样品间AM真菌群落相似性图谱,全部序列归为3个类群,聚类分析显示羊柴根围AM真菌群落具有丰富的遗传多样性,相似性指数最高仅为0.54,说明梁地生境空间异质性对AM真菌遗传多样性有显著影响。受风蚀和水蚀影响,3个样地土壤速效氮差异明显,梁底受侵蚀作用最弱;梁中受侵蚀最强,土壤最贫瘠;球囊霉属Glomus和管柄囊霉属Funneliformis是内蒙古荒漠地区AM真菌优势属,这为进一步筛选高效优势AM菌种,促进荒漠植被恢复提供了依据。
英文摘要	In the present study,we expect to elucidate the genetic diversity and ecological distribution of AM fungi associated with clonal plants Hedysarum leave Maxim under synchronous stress of wind erosion and water erosion,at Qinggeletu in Inner Mongolia,China.Soil samples were collected from 050 cm depth with an interval of 10 cm in the rhizospheres of H.leave Maxim at three different altitude gradients habitats:the foot-slop,middle-slop and top-slop of the terrace in October 2014.The community composition and genetic diversity of AMF were analyzed based on Nested-PCR-DGGE fingerprint and 18S rDNA sequencing.The results of DGGE fingerprint revealed significant differences in the composition,richness,dominance and diversity of the AMF communities between different soil layers and habitats.The richness of AMF community recorded in the 010 cm layer at the middle-slop and top-slop of the terrace was 20,which was the highest among the sampling habitats.The highest diversity index reached 2.88,and was recorded in the 010 cm layer at the middle-slop of the terrace. Sequencing and phylogenetic analysis showed that all the sequences were relegated to 15 species belonging to 5 genus,which were Glomus (10 species),Funneliformis (2 species), Paraglomus (1 species),Rhizophagus (1 species),and Septoglomus (1 species).UPGMA clustering analysis showed that the sequences could be clustered into 3 groups that greatly differed from each other as the highest similarity index was merely 0.54.This meant that the genetic diversity of AMF community in the rhizospheres of H.leave Maxim was significantly influenced by the heterogeneity of the three habitats.Because the synchronous stress of wind and water erosion was different at the three habitats,i.e.,the highest at the middle-slop of terrace and the lightest at the foot-slop,soil available N exhibited significant difference between the three habitats,which consequently significantly influenced the community composition and genetic diversity of AMF.Due to the dominance of Glomus and Funneliformisin the AMF communities in the deserts of Inner Mongolia, high-efficiency species or stains belonging to the two genuses may be selected for improving plant establishment and vegetation rehabilitation in this area.
中文关键词	AM真菌 ; 遗传多样性 ; 羊柴 ; 内蒙古荒漠
英文关键词	PCR-DGGE AM fungal PCR-DGGE genetic diversity Hedysarum leave Maxim desert of Inner Mongolia
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MICROBIOLOGY
WOS研究方向	Microbiology
CSCD记录号	CSCD:5725198
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/234467
作者单位	河北大学生命科学学院, 保定, 河北 071002, 中国
推荐引用方式 GB/T 7714	成斌,胡从从,赵丽莉,等. 羊柴根围AM真菌群落结构和遗传多样性研究[J],2016,39(3):23-30.
APA	成斌,胡从从,赵丽莉,&贺学礼.(2016).羊柴根围AM真菌群落结构和遗传多样性研究.河北农业大学学报,39(3),23-30.
MLA	成斌,et al."羊柴根围AM真菌群落结构和遗传多样性研究".河北农业大学学报 39.3(2016):23-30.