干旱区生态环境知识资源中心(Arid): 荒漠区药用植物黑果枸杞(Lycium ruthencium)的组织培养

Arid

	荒漠区药用植物黑果枸杞(Lycium ruthencium)的组织培养
其他题名	Tissue culture of medicinal plants Lycium ruthencium in arid desert
	王方琳 1; 柴成武 1; 魏小红 2; 马俊梅 1; 李爱德 1; 刘开琳 1; 王昱淇 1; 张莹花 1; 张锦春 1; 杨晓寒 3; 靳承东 3
来源期刊	干旱区资源与环境
ISSN	1003-7578
出版年	2016
卷号	30 期号:10 页码:104-109
中文摘要	试验以荒漠区药用植物黑果枸杞无菌苗的子叶、茎段、胚轴为外植体材料,研究不同激素配比的培养基对不同外植体愈伤组织培养、丛生芽增殖及生根培养的影响,并筛选适宜各个阶段培养的最佳培养基配方。结果表明:子叶是诱导黑果枸杞愈伤组织的最好外植体,在6-BA为0.3mg/L,2,4-D为1.0mg/L时,其愈伤组织诱导率达到最大值95.1%;较高浓度的细胞分裂6-BA对丛生芽增殖具有明显的促进作用,在6-BA为3.0mg/L、KT 0.3mg/L、NAA 0.5mg/L时丛生芽增殖率达到最大值90.2%,且丛生芽数量多、茎干健壮、生长旺盛;在以1/2MS为基本培养基,减少无机盐的浓度时对黑果枸杞丛生芽生根培养具有促进作用,减少Mg SO4一半,并加入NAA0.5mg/L时,丛生芽生根率达到最大值98.2%,且生根时间最早,主根明显、粗壮且根毛数量多。
英文摘要	The experiments using the cotyledons,hypocotyl explants and stem section of Lycium ruthencium as material to research influence of different ratio of hormone on different explant callus culture,the multiple shoot clumps proliferation and rooting culture,and select the best culture medium formula suitable for all stages. The results show that the cotyledon is the best explant to callus induction. While 6 - BA was 0. 3mg /L and 2,4 - D was 1. 0mg /L,the callus induction rate reached a maximum of 95. 1%. The high levels of cell division 6 - BA had obviously promoting effect on multiple shoot clumps proliferation. When 6 - BA was 3. 0mg /L,KT 0. 3mg / L,NAA 0. 5mg /L multiple shoot clumps proliferation rate reached a maximum of 90. 2%,and the multiple shoot clumps number was large,stem was robust with vigorous growth. Using 1 /2 MS as the basic culture medium, and reducing the concentration of inorganic salt had a promoting effects on the Lycium ruthencium multiple shoot clumps growth,reducing half of MgSO4 and joining NAA 0. 5mg /L,the multiple shoot clumps maximum rooting rate reached 98. 2%,and began to take root the earliest time,and taproot was strong and root hairs were thick.
中文关键词	药用植物 ; 黑果枸杞 ; 激素 ; 培养基
英文关键词	medicinal plants Lycium ruthencium hormone medium
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:5813836
来源机构	甘肃农业大学 ; 甘肃省治沙研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/234390
作者单位	1.甘肃省治沙研究所, 甘肃省荒漠化与风沙灾害防治重点实验室, 武威, 甘肃 733000, 中国; 2.甘肃农业大学生命科学技术学院, 兰州, 甘肃 730070, 中国; 3.甘肃省莲花山国家级自然保护区管理局, 临夏, 731500
推荐引用方式 GB/T 7714	王方琳,柴成武,魏小红,等. 荒漠区药用植物黑果枸杞(Lycium ruthencium)的组织培养[J]. 甘肃农业大学, 甘肃省治沙研究所,2016,30(10):104-109.
APA	王方琳.,柴成武.,魏小红.,马俊梅.,李爱德.,...&靳承东.(2016).荒漠区药用植物黑果枸杞(Lycium ruthencium)的组织培养.干旱区资源与环境,30(10),104-109.
MLA	王方琳,et al."荒漠区药用植物黑果枸杞(Lycium ruthencium)的组织培养".干旱区资源与环境 30.10(2016):104-109.