干旱区生态环境知识资源中心(Arid): 固相萃取-高效液相色谱-串联质谱法测定枸杞中9种红色合成色素

Arid

	固相萃取-高效液相色谱-串联质谱法测定枸杞中9种红色合成色素
其他题名	Determination of 9Red Synthetic Pigments in Chinese Wolfberry by Solid-phase Extraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry
	粟有志 1; 刘俊 2; 李艳美 1; 马志愿 3; 雷红琴 1; 罗琼 1
来源期刊	分析科学学报
ISSN	1006-6144
出版年	2016
卷号	32 期号:6 页码:846-850
中文摘要	建立了固相萃取-高效液相色谱-串联质谱(HPLC-MS/MS)联用同时测定枸杞中酸性红1、酸性红9、诱惑红等9种红色合成色素的分析方法。样品经乙腈-甲醇(5∶5,V/V)提取,用Oasis WAX固相萃取柱净化,目标化合物以Zorbax Eclipse Plus C_(18)柱(100*3.0mm,1.8mum)分离,采用乙腈-10mmol/L乙酸铵为流动相梯度洗脱,采用电喷雾负离子源(ESI-)、多重反应监测模式(MRM)检测,基质匹配外标法进行定量。9种红色合成色素在各自线性范围内相关系数(r~2)均大于0.992;方法的定量限以信噪比(S/N)≥10计为0.01~3.00mg/kg,3个加标水平(1、2、10倍定量限)下,回收率为82.1%~99.8%,相对标准偏差(RSDs)为4.6%~16.1%。该方法准确度好、灵敏度高、重现性好,适用于枸杞中红色合成色素的测定。
英文摘要	A method for the simultaneous determination of nine red synthetic pigments(acid red 1,acid red 9,allura red,etc)in Chinese wolfberry was developed using solid-phase extraction(SPE)- high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The samples were extracted by acetonitrile-methanol(5∶5,V/V),and then cleaned up by Oasis WAX solid-phase extractor. The analytes were separated on a Zorbax Eclipse Plus C_(18) column(100*3.0mm,1.8mum)by gradient elution with acetonitrile-10mmol/L ammonium acetate as the mobile phases.9red synthetic pigments were determined by electrospray negative ion source(ESI~-)with multiple reaction monitoring mode (MRM),and the quantitative analysis was carried out by matrix-matched external standard method.The correlation coefficients of linear calibration curves were over 0.992in the corresponding concentration ranges.The limits of quantification of 9red synthetic pigments(LOQs,S/N≥10)were in the range of 0.01- 3.0 mg/kg;the average recoveries were in the range of 82.1%-99.8% with the relative standard deviations(RSDs)ranging from 4.6%to 16.1%at the spiked three levels(1,2,10LOQ).This method was proven to be highly sensitive,accurate and precise,and suitable for the monitoring of the red synthetic pigments in Chinese wolfberry.
中文关键词	固相萃取(SPE) ; 红色合成色素 ; 枸杞 ; 高效液相色谱-串联质谱
英文关键词	Solid-phase extraction Red synthetic pigments Chinese wolfberry HPLC-MS/MS
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:5879350
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/234167
作者单位	1.伊犁出入境检验检疫局综合技术服务中心, 伊宁, 新疆 835000, 中国; 2.新疆出入境检验检疫局技术中心, 乌鲁木齐, 新疆 830063, 中国; 3.伊犁师范学院化学与生物科学学院, 伊宁, 新疆 835000, 中国
推荐引用方式 GB/T 7714	粟有志,刘俊,李艳美,等. 固相萃取-高效液相色谱-串联质谱法测定枸杞中9种红色合成色素[J],2016,32(6):846-850.
APA	粟有志,刘俊,李艳美,马志愿,雷红琴,&罗琼.(2016).固相萃取-高效液相色谱-串联质谱法测定枸杞中9种红色合成色素.分析科学学报,32(6),846-850.
MLA	粟有志,et al."固相萃取-高效液相色谱-串联质谱法测定枸杞中9种红色合成色素".分析科学学报 32.6(2016):846-850.