干旱区生态环境知识资源中心(Arid): 沙漠干热环境创伤失血性休克大鼠继发性肺损伤时一氧化氮合酶等变化研究

Arid

	沙漠干热环境创伤失血性休克大鼠继发性肺损伤时一氧化氮合酶等变化研究
其他题名	The studies on the changes of nitric oxide synthase in the lung tissue of traumatic hemorrhagic shock rats under dry heat environment of desert and their relations to the secondary lung injuris
	李瑞; 刘江伟; 钱建辉; 钱若筠; 张琼; 郑树涛; 杨帆
来源期刊	中华急诊医学杂志
ISSN	1671-0282
出版年	2015
卷号	24 期号:2 页码:163-168
中文摘要	目的研究沙漠干热环境中创伤失血性休克大鼠肺脏发生继发性损伤时，肺组织病理改变与肺组织一氧化氮(NO)浓度、诱导型一氧化氮合酶(iNOS) mRNA表达量的变化。方法在兰州军区乌鲁木齐总医院动物实验科西北特殊环境人工实验舱中，140只雄性SD大鼠随机（随机数字法）分为常规环境创伤失血性休克组（常温组）和干热环境创伤失血性休克组（干热组），每组又分别设对照组、休克后0、0.5、1、1.5、2、3 h组7个亚组，每组10只。对照组不做处理，干热组预热60 min，麻醉、固定，使用静脉留置针行右颈动、静脉和右股动脉插管，稳定10 min后,利用2500 g铁轮于30 cm高度击中SD大鼠左下肢股骨中上段造成粉碎性骨折，致伤后简易包扎伤口，经右股动脉放血使MAP维持在（35 5） mmHg (1 mmHg =0.133 kPa)，继续监测60 min后进行复苏。成功建立创伤失血性休克模型后，依据分组相应时间点，打开胸腔，留取肺灌洗液、肺组织，常温组除预热外其余操作相同。HE染色观察肺组织病理学改变和一步法检测肺组织NO质量摩尔浓度，荧光定量PCR检测iNOS mRNA表达量的变化。对计量资料进行t检验与单因素方差分析，各指标间进行Pearson相关分析。结果病理观察可见干热组各时间点肺病理损伤较常温组病理损伤、蛋白渗出严重且肺组织病理学评分较高。两组肺组织匀浆NO质量摩尔浓度总体比较差异具有统计学意义（t=2.472，P<0.05），干热组内各时间点比较差异具有统计学意义(F = 6. 77, P<0.01),同一时间点干热组数值均大于常温组数值，干热组在2 h达到最大值(3. 35 0.23) mumol/g较常温组峰值出现早。两组iNOS mRNA表达量总体比较t检验差异具有统计学意义（t=3.619， P<0.01），干热组组内各时间点比较差异具有统计学意义(F=12.34， P< 0.01),同一时间点干热组数值均大于常温组，干热组1.5 h时峰值出现，常温组则在2 h时开始增加。两组肺组织匀浆NO质量摩尔浓度与iNOS mRNA表达量呈正相关(r=0.680、r=0.376)，iNOS mRNA表达量与肺损伤病理学评分呈正相关(r =0. 846、r =0. 899)。结论在沙漠干热环境中创伤失血性休克继发性肺损伤时，肺脏损伤较常温环境下严重、较早，NO、iNOS在沙漠干热环境创伤失血性休克继发性肺损伤过程中起重要作用。
英文摘要	Objective To study the pathological changes and expressions of NO and iNOS mRNA in the lung tissue of traumatic hemorrhagic shock rats under dry heat environment of desert and their relations to the lung injury. Methods A total of 140 male SD rats were randomly (random number) ivided into the room temperature (25℃) environment traumatic hemorrhagic shock group ( room temperature group) and the dry heat traumatic hemorrhagic shock groups （dry heat group, temperature 40℃，humidity 10%），respectively, and each groups was further randomly divided into 7 subgroups : the control subgroup, post shock subgroups at 0,0. 5,1, 1.5, 2and 3 h (n = 10 in each subgroup). The rats of control subgroup were not treated,and rats of dry heat group were placed in dry heat environment for 60 min,then anesthetized, fixed,and insertion of intravenous indwelling needles and catherization of right carotid artery,jugular vein and the right femoral artery were performed. After stabilization for 10 min, 2500 g iron wheel was used to be dropped from 30 m height and vertically hit the upper left femoral of SD rats in order to make comminuted fracture, wounds were quickly dressed after injury. Exsanguination from right femoral artery was kept until MAP maintained at (35 5) mmHg, and resuscitation was carried out after continue monitoring for 60 min. After the establishment of traumatic hemorrhagic shock model in each environment, the rats were sacrificed at given intervals, and thoracotomy was performed to take broncho - alveolar lavage fluid ( BALF) and lung tissue. Pathological changes of lung tissues were observed by using HE staining and NO concentration of lung tissue was detected by one-step method,and changes of the iNOS mRNA expressions were detected by using fluorescence quantitative PCR. Then t test, ANOVA and Pearson correlation analysis were used for the data analysis. Results The pathological change in dry heat group at each interval was more severe, and pulmonary histopathological injury score was higher, and the protein exudation was more profuse compared with the room temperature group. NO concentration in lung tissue homogenate of dry heat group was higher than that of room temperature group （t = 2. 472，P < 0. 05）， and the difference in NO level between different intervals within the dry heat group was statistically significant （ F = 6. 77,P < 0. 01 ）. The NO concentration in dry heat group reached its maximum at 2 h (3. 35 0. 23) mumol / g and the peak value emerged sooner than that in room temperature group. The difference was statistically significant in overall expression of iNOS mRNA between two groups analyzed with t test (t = 3. 619, P <0. 01), and there was statistically significant difference between intervals within the dry heat group (F = 12. 34, P <0. 01 ). The values of iNOS mRNA in the dry heat group were higher than those in the room temperature group at the same given intervals, and the peak value appears at 1. 5 h in dry heat group, and the room temperature group it began to increase at 2 h. The concentration of NO and the expression of iNOS mRNA were positively correlated with each other in two groups (r = 0. 680,r = 0. 376 ). The expression of iNOS mRNA and lung histopathological injury score was positively correlated in two groups (r = 0. 846, r = 0. 899). Conclusions When traumatic hemorrhagic shock occurred in the dry heat desert environment, the lung injury was more severe and appeared sooner than that in the room temperature environment. NO and iNOS played important roles in the secondary lung injury in the wake of traumatic hemorrhagic shock in rats under the dry heat environmengt of desert.
中文关键词	干热环境 ; 创伤失血性休克 ; 肺脏 ; 继发性肺损伤 ; 病理学肺损伤评分 ; 肺灌洗液总蛋白 ; 诱导型一氧化氮合酶 ; 一氧化氮
英文关键词	Dry heat environment Traumatic hemorrhagic shock Pulmonary Secondary lung injury Pathological lung injury score Lung lavage total protein Inducible nitric oxide synthase Nitric oxide
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:5351026
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/233839
作者单位	兰州军区乌鲁木齐总医院, 新疆特殊环境医学重点实验室, 乌鲁木齐, 新疆 830000, 中国
推荐引用方式 GB/T 7714	李瑞,刘江伟,钱建辉,等. 沙漠干热环境创伤失血性休克大鼠继发性肺损伤时一氧化氮合酶等变化研究[J],2015,24(2):163-168.
APA	李瑞.,刘江伟.,钱建辉.,钱若筠.,张琼.,...&杨帆.(2015).沙漠干热环境创伤失血性休克大鼠继发性肺损伤时一氧化氮合酶等变化研究.中华急诊医学杂志,24(2),163-168.
MLA	李瑞,et al."沙漠干热环境创伤失血性休克大鼠继发性肺损伤时一氧化氮合酶等变化研究".中华急诊医学杂志 24.2(2015):163-168.