干旱区生态环境知识资源中心(Arid): 塔克拉玛干沙生植物来源放线菌抗铜绿假单胞菌活性的筛选及菌株38-7L-1活性产物的研究

Arid

	塔克拉玛干沙生植物来源放线菌抗铜绿假单胞菌活性的筛选及菌株38-7L-1活性产物的研究
其他题名	Endophytic actinomycetes from psammophytes of Taklamakan Desert against Pseudomonas aeruginosa and study on bioactive product of strain 38-7L-1
	陈川 1; 刘佳萌 1; 蒋忠科 1; 李小俊 1; 刘少伟 1; 庹利 1; 戴素娟 1; 杨桂柳 2; 王飞飞 1; 黄大林 3; 姜明国 2; 孙承航 1
来源期刊	中国抗生素杂志
ISSN	1001-8689
出版年	2015
卷号	40 期号:2 页码:81-87,115
中文摘要	目的筛选来自塔克拉玛干沙漠沙生植物对铜绿假单胞菌具有抑制活性的内生放线菌菌株,对拟诺卡菌属菌株38-7L-1发酵液中活性化合物进行分析预测、分离纯化和结构验证。方法采用琼脂平板法进行抗菌活性筛选;基于16S rRNA基因序列对菌株38-7L-1进行初步分类鉴定;基于菌株38-7L-1 PKS I基因的KS域序列分析结果,结合活性峰的液-质联用数据,比对微生物天然产物数据库,预测活性化合物201的结构;通过色谱方法纯化化合物201;结合光谱数据分析及文献比对,确定和验证化合物201的结构。结果从81株沙生植物内生放线菌中获得7株活性菌株,包括链霉菌属菌株4株、考克菌属菌株1株、多形孢菌属菌株1株和拟诺卡菌属菌株1株。其中菌株38-7L-1活性最强并具有I型聚酮合酶基因,16S rRNA基因序列分析表明该菌株与Nocardiopsis alba DSM 43377~T(X97883)的相似率为100%。菌株38-7L-1抗铜绿假单胞菌活性次级代谢产物,化合物201为十六元大环内酯类化合物,蔷薇霉素。结论首次从拟诺卡菌中分离到常见于小单孢菌的蔷薇霉素,多策略组合对次级代谢产物结构预测具有指导意义。
英文摘要	Objective To screen endophytic actinomycetes isolated from psammophytes collected in Taklamakan Desert with inhibitory activity against Pseudomonas aeruginosa, and predict, isolate, purify and identificate the structure of bioactive component produced by Nocardiopsis strain 38-7L-1. Methods The fermentation broths of 81 strains were screened by agar disc diffusion, strain 38-7L-1 was identified on the basis of sequence analysis of 16S rRNA gene, a bioactive secondary metabolite named as compound 201 produced by the strain 38-7L-1 was predicted by comprehensive analysis of type I PKS gene, UV and MS data provided by HPLC-DAD-MS and microbial product database; compound 201 was purified by different chromatography and its chemical structure was determined by spectral data analysis and comparison with that of published data. Results 7 strains including 4 Streptomyces strains and 3 rare actinomycetes strains were obtained as positive strains, among them, Nocardiopsis sp. 38-7L-1 showed the strongest inhibitory activity against Pseudomonas aeruginosa; predicted bioactive component produced by the strain was purified and proved to be rosamicin, a known 16-membered macrolide. Conclusion Combination of different strategy involved in discovery of antibiotic has great significance for dereplication of known antibiotic and finding new antibiotics. Rosamicin, normally produced by the genus Micromonospra, was found for the first time in the fermentation broth of Nocardiopsis sp.
中文关键词	塔克拉玛干沙漠 ; 植物内生放线菌 ; 铜绿假单胞菌 ; 拟诺卡菌属 ; 蔷薇霉素
英文关键词	Taklamakan Desert Endophytic actinomycetes Pseudomonas aeruginosa Nocardiopsis Rosamicin
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:5354969
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/233675
作者单位	1.中国医学科学院北京协和医学院医药生物技术研究所, 北京 100050, 中国; 2.广西民族大学海洋与生物技术学院, 广西高校微生物与植物资源利用重点实验室, 南宁, 广西 530008, 中国; 3.桂林医学院, 桂林, 广西 541004, 中国
推荐引用方式 GB/T 7714	陈川,刘佳萌,蒋忠科,等. 塔克拉玛干沙生植物来源放线菌抗铜绿假单胞菌活性的筛选及菌株38-7L-1活性产物的研究[J],2015,40(2):81-87,115.
APA	陈川.,刘佳萌.,蒋忠科.,李小俊.,刘少伟.,...&孙承航.(2015).塔克拉玛干沙生植物来源放线菌抗铜绿假单胞菌活性的筛选及菌株38-7L-1活性产物的研究.中国抗生素杂志,40(2),81-87,115.
MLA	陈川,et al."塔克拉玛干沙生植物来源放线菌抗铜绿假单胞菌活性的筛选及菌株38-7L-1活性产物的研究".中国抗生素杂志 40.2(2015):81-87,115.