干旱区生态环境知识资源中心(Arid): 人居和荒漠草原生态条件下小花棘豆叶片蛋白质表达谱分析

Arid

	人居和荒漠草原生态条件下小花棘豆叶片蛋白质表达谱分析
其他题名	Analysis of Protein Expression Profile of Oxytropis glabra under Adverse Environment
	周攀 1; 吴道长 1; 王德军 2; 李国中 3; 尉亚辉 1
来源期刊	中国草地学报
ISSN	1673-5021
出版年	2015
卷号	37 期号:1 页码:38-44
中文摘要	利用双向电泳技术,对比两种生境下(A地为公园,B地为荒漠草原)小花棘豆的蛋白质差异表达谱,选取其中的差异蛋白点进行MADLI-TOF-MS鉴定,获得的PMF数据经Mascot软件在NCBI NR数据库上检索分析后确认蛋白种类,结果表明:相对A地,B地小花棘豆光合作用相关调控酶类表达下调,而光合作用结构蛋白如叶绿素球蛋白表达上调;B地小花棘豆光合速率下降,可能是受光合调控酶表达下调的影响;B地ATP合成相关蛋白的表达上调,说明在干旱、高盐、氮磷含量偏低的环境下生长可能需要消耗更多能量。另外,B地小花棘豆中发现一些与胁迫相关蛋白出现上调或者特异性表达,这些蛋白的表达可能跟小花棘豆能够适应逆境有着密切的关系。
英文摘要	Two habitats of Oxytropis glabra, park (place A) and desert steppe (place B), were selected. The differences in protein expression profile of plants from the above two sites were compared using two-dimensional electrophoresis technology and the different protein spots were identified by MADLI-TOF-MS. The identities of the proteins were confirmed by Mascot software against the NCBI NR database. The regulating enzymes of photosynthesis of Oxytropis glabra in place B decreased compared to the sample in place A. But the structural proteins of photosynthesis such as chlorophyll and globulin were up-regulated. Photosynthetic rate of Oxytropis glabra decreased in place B, probably because of the reduced expression of photosynthetic regulation enzyme. Furthermore, the abundance of ATP synthesis-related proteins in place B increased, which indicated the samples in drought, high salinity, low nitrogen and phosphorus content of the growth environment need to consume more energy. In addition, it was found that some up-regulated proteins in Oxytropis glabra from place B were specific proteins associated with stress. The expression of these proteins may enable Oxytropis glabra to live in adverse environment.
中文关键词	人居生态条件 ; 荒漠生态条件 ; 叶片 ; 蛋白质 ; 表达谱
英文关键词	Living environment Desert grassland environment Leaves Protein Expression profile
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:5356067
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/233649
作者单位	1.西北大学生命科学院, 西部资源与现代生物技术陕西省省部共建教育部重点实验室;;陕西省生物技术重点实验室, 西安, 陕西 710069, 中国; 2.内蒙古阿拉善左旗腾格里额里斯动物卫生监督站, 内蒙古 750314, 中国; 3.内蒙古阿拉善左旗动物卫生监督所, 内蒙古 750300, 中国
推荐引用方式 GB/T 7714	周攀,吴道长,王德军,等. 人居和荒漠草原生态条件下小花棘豆叶片蛋白质表达谱分析[J],2015,37(1):38-44.
APA	周攀,吴道长,王德军,李国中,&尉亚辉.(2015).人居和荒漠草原生态条件下小花棘豆叶片蛋白质表达谱分析.中国草地学报,37(1),38-44.
MLA	周攀,et al."人居和荒漠草原生态条件下小花棘豆叶片蛋白质表达谱分析".中国草地学报 37.1(2015):38-44.