干旱区生态环境知识资源中心(Arid): 固相萃取-液相色谱-串联质谱法检测血液中海洛因及其代谢产物

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	固相萃取-液相色谱-串联质谱法检测血液中海洛因及其代谢产物
其他题名	Determination of Heroin and Its Metabolites in Addicts’Blood by Solid Phase Extraction and LC-MS/MS
	张松 1; 曹峻华 1; 刘文文 2
来源期刊	质谱学报
ISSN	1004-2997
出版年	2015
卷号	36 期号:4 页码:367-371
中文摘要	建立了固相萃取-液相色谱-串联质谱(SPE-LC-MS/MS)法分析吸毒者血样中的O~6-单乙酰吗啡、吗啡、3-beta-D-葡萄糖醛酸吗啡。以3-beta-D-葡萄糖醛酸吗啡-d_3为内标,选取Oasis HLB为固相萃取柱, Atlantis~(TM) dC18(150 mm3.9 mm5mum)色谱柱为分析柱,采用甲醇-0.1%甲酸水溶液为流动相进行梯度洗脱;采用电喷雾正离子电离源,多反应监测(MRM)扫描方式,通过两对母离子/子离子和保留时间定性定量检测目标化合物。结果表明:血样中O_6-单乙酰吗啡、吗啡、3-beta-D-葡萄糖醛酸吗啡的检测限为3~5mug/L,线性关系良好,相关系数均在0.997 5以上;添加50、500、1 000mug/L加标水平后,O~6-单乙酰吗啡、吗啡、3-beta-D-葡萄糖醛酸吗啡的平均回收率为80.6%~98.9%,准确度为-7.3%~9.1%。该方法具有较高的灵敏度和选择性,可用于实际案件中海洛因及其代谢产物的检测。
英文摘要	O~6-Monoacetylmorphine,morphine,morphine-3-beta-D-glucuronide in addicts’ blood samples were analyzed by solid phase extraction(SPE)and liquid chromatography- tandem mass spectrometry(LC-MS/MS).The samples were extracted by extraction column(Oasis HLB),and then were flushed into the analytical column(Atlantis~(TM) dC18(150 mm3.9 mm5mum))with methanol and water containing formic acid as mobile phase and morphine-3-beta-D-glucuronide-d_3 as internal standard.The positive electric spray ionization,multiple reaction monitoring(MRM)mode were applied to analyze target compounds.Identification was based on the compound’s retention time and two pairs of precursor-to-product ion transitions.The results show that the limit of detections are 3-5 mug/L for morphine,O~6-monoacetylmorphine and morphine-3-beta-Dglucuronide. The linear relationship is well,and the linear correlation coefficient is greater than 0.997 5.The average recoveries are 80.6%-98.9% with the spiked levels of 50,500,1 000mug/L,and the accuracies are-7.3%-9.1%.The method has high sensitivity and selectivity,which is suitable for analyzing the heroin and its metabolites in addicts’blood samples.
中文关键词	液相色谱-串联质谱法(LC-MS/MS) ; 固相萃取(SPE) ; O~6-单乙酰吗啡 ; 吗啡 ; 3-beta-D-葡萄糖醛酸吗啡
英文关键词	liquid chromatography-tandem mass spectrometry (LC-MS/MS) solid phase extraction (SPE) O~6-monoacetylmorphine morphine morphine-3-beta-D-glucuronide
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:5473449
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/233648
作者单位	1.重庆市公安局交通管理局, 重庆 400054, 中国; 2.重庆市公安局物证鉴定中心, 重庆 400021, 中国
推荐引用方式 GB/T 7714	张松,曹峻华,刘文文. 固相萃取-液相色谱-串联质谱法检测血液中海洛因及其代谢产物[J],2015,36(4):367-371.
APA	张松,曹峻华,&刘文文.(2015).固相萃取-液相色谱-串联质谱法检测血液中海洛因及其代谢产物.质谱学报,36(4),367-371.
MLA	张松,et al."固相萃取-液相色谱-串联质谱法检测血液中海洛因及其代谢产物".质谱学报 36.4(2015):367-371.