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	柴达木地区野生黑果枸杞种群遗传多样性的AFLP分析
其他题名	Population genetic diversity of wild Lycium ruthenicum in Qaidam inferred from AFLP markers
	王锦楠 1; 陈进福 2; 陈武生 2; 周新洋 3; 许东 1; 李际红 1; 亓晓 1
来源期刊	植物生态学报
ISSN	1005-264X
出版年	2015
卷号	39 期号:10 页码:1003-1011
中文摘要	采用扩增片段长度多态性(AFLP)分子标记技术对青海省柴达木地区5个野生黑果枸杞(Lycium ruthenicum)种群的120份样品的遗传多样性进行分析。结果表明: 柴达木地区野生黑果枸杞具有很高的遗传多样性, 9对选扩引物共得到1 691条清晰条带, 其中多态性条带1 678条, 多态性变异率为99.23%, 种群间的有效等位基因数为1.471 2, Nei’s基因多样性为0.324 5, Shannon信息指数为0.436 7。分子方差分析(AMOVA)结果表明: 柴达木地区5个黑果枸杞种群的遗传变异主要存在于种群内部(92%), 种群间的遗传分化较小(8%, 遗传分化系数0.08)。黑果枸杞种群间的遗传相似系数介于0.970 9-0.992 2之间, 平均值为0.983 5。种群间的聚类及Mantel检验(gamma = 0.336 8, p = 0.806 4)均表明柴达木地区黑果枸杞种群地理距离与遗传距离之间的相关性不明显; 黑果枸杞个体间的聚类表明同一种群的个体不能完全聚在一起。对同一种源的遗传多样性分析发现, 诺木洪奥斯勒草场的种源内部的遗传变异更为丰富, 这或许可以推断诺木洪可能为柴达木地区野生黑果枸杞种质资源的中心产区。
英文摘要	Aims Lycium ruthenicum is a perennial shrub plant. It grows among rocks in deserts, and its populations are spatially scattered in the distribution range. The objectives of this study were investigating the population genetic diversity and genetic structure of L. ruthenicum in Qaidam and determining relationships between genetic diversity and environmental factors. Methods We analyzed the genetic diversity of 120 individuals from 5 natural populations using amplified fragment length polymorphism(AFLP)markers. Important findings We obtained 1 691 unambiguous bands from the nine pairs of selected primers, 1 678 bands(99.23%)of which were polymorphic across all individuals. At the species level, the Nei’s gene diversity was 0.364 9, and the Shannon’s information index was 0.542 2. Analysis of molecular variance(AMOVA)analysis indicated that most variations(92.0%)existed within populations, with only a small proportion of total variations residing among populations(8.0%, genetic differentiation coefficient is 0.08). The genetic similarity among the populations ranged from 0.970 9 to 0.992 2 with an average of 0.983 5. Results of the Mantel test(gamma = 0.336 8, p = 0.806 4)and unweighted pair group method with arithmetic mean(UPGMA)cluster of the Qaidam populations indicated that the correlation between geographical distance and genetic distance was not significant. Principal coordinate analysis of all sampled individuals showed that individuals from each population did not cluster together. The genetic diversity of the same population indicated that the L. ruthenicum of Nomhon had high level of genetic variations, which indicated Nomhon is the center of the Qaidam wild L. ruthenicum populations.
中文关键词	黑果枸杞 ; 柴达木地区 ; 遗传多样性
英文关键词	AFLP Lycium ruthenicum Qaidam genetic diversity AFLP
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRONOMY
WOS研究方向	Agriculture
CSCD记录号	CSCD:5551703
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/233630
作者单位	1.山东农业大学林学院农业生态与环境重点实验室, 泰安, 山东 271018, 中国; 2.青海省林业科学研究所, 西宁, 青海 810016, 中国; 3.山东大学药学院, 济南, 山东 250012, 中国
推荐引用方式 GB/T 7714	王锦楠,陈进福,陈武生,等. 柴达木地区野生黑果枸杞种群遗传多样性的AFLP分析[J],2015,39(10):1003-1011.
APA	王锦楠.,陈进福.,陈武生.,周新洋.,许东.,...&亓晓.(2015).柴达木地区野生黑果枸杞种群遗传多样性的AFLP分析.植物生态学报,39(10),1003-1011.
MLA	王锦楠,et al."柴达木地区野生黑果枸杞种群遗传多样性的AFLP分析".植物生态学报 39.10(2015):1003-1011.