干旱区生态环境知识资源中心(Arid): 松叶猪毛菜干旱胁迫下实时定量PCR内参基因的筛选

Arid

	松叶猪毛菜干旱胁迫下实时定量PCR内参基因的筛选
其他题名	Reference Gene Selection for Real-Time Quantitative PCR in Salsola laricifolia under Soil Drought Stress
	闻志彬; 张明理
来源期刊	植物生理学报
ISSN	2095-1108
出版年	2015
卷号	51 期号:11 页码:2031-2038
中文摘要	实时荧光定量PCR (qRT-PCR)是研究植物基因定量表达的常用手段之一, 选择合适的内参基因是获得可信定量结果的前提。本文以不同干旱胁迫处理下松叶猪毛菜的叶片为材料, 应用qRT-PCR技术分析5个常用候选管家基因(18S rRNA、 alpha-tubulin、beta-actin、EF1-alpha和GAPDH)的表达变化, 利用geNorm和NormFinder软件筛选出在干旱胁迫下表达最稳定的内参基因。结果表明, 以不同干旱胁迫处理的所有样品作为总样品池进行内参基因筛选时, 候选内参基因表达稳定性顺序依次是beta-actin>alpha-tubulin>GAPDH>EF1-alpha>18S rRNA。对同一干旱处理的样品进行内参基因筛选时, 5个候选内参基因表达稳定性存在明显差异, 其中beta-actin表达稳定度最高。因此, beta-actin是研究松叶猪毛菜干旱胁迫下基因表达最合适的内参基因。
英文摘要	Real-time quantitative PCR (qRT-PCR) is one of the common technologies used for gene expression analysis. Selection of a suitable reference gene is a prerequisite to obtain the reliable results. In this study, five housekeeping genes including ribosomal RNA 18S (18S rRNA), alpha-tubulin, beta-actin, elongation factor 1 alpha (EF1-alpha) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) of the desert plant, Salsola laricifolia, were selected as candidate reference genes in the qRT-PCR, and two softwares, geNorm and NormFinder, were used to evaluate their expression stabilities in the leaves of S. laricifolia under soil drought stress in order to obtain a reliable reference gene. The results showed that there were significant differences among the five candidate reference genes under different soil drought stress treatments. And the order of expression stability was beta-actin> alpha-tubulin>GAPDH>EF1-alpha>18S rRNA. While this order of expression stability was different under the same soil drought stress treatment, but the expression stability of beta-actin was highest. In a word, beta-actin was the most stable gene for analysis gene expression in S. laricifolia under soil drought stress.
中文关键词	松叶猪毛菜 ; 实时荧光定量PCR ; 内参基因 ; 干旱胁迫 ; 藜科
英文关键词	Salsola laricifolia real-time quantitative PCR reference gene soil drought stress Chenopodiaceae
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:5601776
来源机构	中国科学院新疆生态与地理研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/233625
作者单位	中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐, 新疆 830011, 中国
推荐引用方式 GB/T 7714	闻志彬,张明理. 松叶猪毛菜干旱胁迫下实时定量PCR内参基因的筛选[J]. 中国科学院新疆生态与地理研究所,2015,51(11):2031-2038.
APA	闻志彬,&张明理.(2015).松叶猪毛菜干旱胁迫下实时定量PCR内参基因的筛选.植物生理学报,51(11),2031-2038.
MLA	闻志彬,et al."松叶猪毛菜干旱胁迫下实时定量PCR内参基因的筛选".植物生理学报 51.11(2015):2031-2038.