干旱区生态环境知识资源中心(Arid): 含好氧不产氧光合基因簇和Xanthorhodopsin-like基因的Sphingomonas sp. MIM37:基因组及光促生长分析

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	含好氧不产氧光合基因簇和Xanthorhodopsin-like基因的Sphingomonas sp. MIM37:基因组及光促生长分析
其他题名	Sphingomonas sp. MIM37 possessing aerobic anoxygenic photosynthetic gene cluster and xanthorhodopsin-like gene: its genome draft and growth stimulation by illumination
	张星星; 刘亚鹏; 袁博; 赵吉睿; 王瑞刚; 冯福应
来源期刊	微生物学通报
ISSN	0253-2654
出版年	2015
卷号	42 期号:8 页码:1520-1528
中文摘要	[目的]菌株MIM37为具有两种光能利用途径的光合异养细菌,分析其基因组和光照对生长的影响,为理解光能利用途径、光营养生物多样性以及光合作用的进化和功能等提供线索。 [方法]采用平板涂布划线法分离菌株,结合形态观察及16S rRNA基因和光合基因序列同源性与系统发育分析进行初步分类鉴定;以分光光度法和荧光显微观察法测定光照和黑暗培养下培养液细胞浓度和单细胞体积;构建片段长度为300-500 bp的Illumina PE文库,以Illumina Hiseq2000进行基因组测序,以SOAPdenovo和GapCloser组装序列,以RAST在线软件注释基因组。[结果]从内蒙古腾格里沙漠天鹅湖表层水中分离获得一株细菌MIM37,经16S rRNA基因、pufM和视紫质基因同源性和系统发育分析均显示其与Sphingomonas属亲缘关系最为密切;相对黑暗培养,光照刺激下的最大细胞浓度和单细胞体积大小分别提高了1.2和5.6倍;基因组注释显示MIM37代谢途径多样,含典型好氧菌的呼吸电子传递链,具有完整的好氧不产氧细菌的光合基因簇及Xanthorhodopsin-like视紫质蛋白基因,合成铁载体,还原重金属,降解微囊藻毒素和多环芳烃类等。[结论]MIM37属于Sphingomonas属,具有两种光能利用途径,光照可明显促进其生长,多样的代谢模式可能使其在自然环境中极具竞争力、分布广泛并具有应用于修复环境污染的潜力。
英文摘要	[Objective] The genome of the photoheterotrophic bacterium MIM37, possessing anoxygenic photosynthetic gene cluster (PGC) and xanthorhodopsin-like gene, was sequenced and the stimulated growth by illumination was determined, which is helpful in shedding light upon the diversity of photosynthetic pathway and phototrophic bacteria, and the evolution and role of photosynthesis. [Methods] The isolation was performed by the traditional plate spread and streak. To classify and identify the isolate preliminarily, the morphological feature and, the homology and phylogeny of 16S rRNA gene, pufM and rhodopsin genes were analyzed. Spectrophotometry and fluorescence microscopy were employed separately to reveal the concentration and size shifts of the cell cultured under illumination or dark. Illumina PE library of 300-500 bp fragments was constructed for Illumina Hiseq2000 genome sequencing. Sequences were assembled through the software of SOAPdenovo and GapCloser and the online software of RAST was used to annotate. [Results] We obtained a bacterial strain MIM 37 from Lake Swan in Desert Tenggeli in Inner Mongolia. The homology and phylogeny analysis based on 16S rRNA gene, pufM and rhodopsin genes respectively, showed that the strain most closely related to the members of Sphingomonas genus. Compared with the dark condition, light condition increased the maximum of the cell concentration and volume by 1.2 and 5.6 folds respectively. The genome draft sequence demonstrated that MIM37 has diverse metabolic pathways with electron transport chain of the typical aerobic respiration, intact aerobic phototrophic photosynthetic gene cluster and xanthorhodopsin-like genes, siderophore biosynthesis, heavy metal reduction, the degradation of microcystin and polyaromatic hydrocarbon and etc. [Conclusion] The strain MIM37 is a member of the genus Sphingomonas and possesses two different phototrophic pathways. Light may stimulate its growth markedly. The diverse metabolic pathways may be helpful for the strain in the survival and wide distribution in natural ecosystems, and make strain MIM37 useful in environmental pollutes bioremediation.
中文关键词	好氧不产氧光合细菌 ; 光合基因簇 ; 视紫质 ; 基因组
英文关键词	16S rRNA pufM Aerobic anoxygenic phototrophic bacteria Photosynthetic gene cluster Rhodopsin 16S rRNA pufM Genome
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MICROBIOLOGY
WOS研究方向	Microbiology
CSCD记录号	CSCD:5490139
来源机构	内蒙古农业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/233458
作者单位	内蒙古农业大学生命科学学院,应用与环境微生物研究所, 呼和浩特, 内蒙古 010018, 中国
推荐引用方式 GB/T 7714	张星星,刘亚鹏,袁博,等. 含好氧不产氧光合基因簇和Xanthorhodopsin-like基因的Sphingomonas sp. MIM37:基因组及光促生长分析[J]. 内蒙古农业大学,2015,42(8):1520-1528.
APA	张星星,刘亚鹏,袁博,赵吉睿,王瑞刚,&冯福应.(2015).含好氧不产氧光合基因簇和Xanthorhodopsin-like基因的Sphingomonas sp. MIM37:基因组及光促生长分析.微生物学通报,42(8),1520-1528.
MLA	张星星,et al."含好氧不产氧光合基因簇和Xanthorhodopsin-like基因的Sphingomonas sp. MIM37:基因组及光促生长分析".微生物学通报 42.8(2015):1520-1528.