干旱区生态环境知识资源中心(Arid): 塔克拉马干沙漠存在超耐beta-内酰胺环抗生素微生物的证据及对一个新物种的生物化学特性描述

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	塔克拉马干沙漠存在超耐beta-内酰胺环抗生素微生物的证据及对一个新物种的生物化学特性描述
其他题名	Evidence of The Presence of Bacteria Highly Resistant to beta-Lactam Antibiotics in Taklimakan Desert and Biochemical Characterization of Paramesorhizobium deserti gen. nov., sp. nov
	罗雪松 ; 潘云帆 ; 石晶晶 ; 代俊 ; 侯莎 ; 方呈祥 ; 彭芳
来源期刊	生物化学与生物物理进展
ISSN	1000-3282
出版年	2015
卷号	42 期号:1 页码:56-64
中文摘要	在对塔克拉玛干沙漠土著微生物进行研究过程中,发现10株细菌能够在含有1 000 mg/L氨苄青霉素的0.1*TSB 平板上生长. 这些微生物具有广泛的beta- 内酰氨类抗生素耐受性. 它们均属于变形杆菌纲,其中5个菌株鉴定为条件致病菌 Stenotrophomonas matltophila,还有4个菌株与Mesorhizobium amorphae亲缘关系很近.有趣的是,菌株A-3-E~T与已知物种同源性低,通过基于生物化学方法的多相分类学研究,我们将其确定为一个新属,并命名为 Paramesorhizobium desertii gen.nov., sp. nov.,发现高浓度的beta- 内酰胺类抗生素,如1 000 mg/L的唑啉头孢菌素或250 mg/L 的头孢氨噻肟,依然无法抑制其生长. 此外,该菌株还能耐受所测试28种抗生素中的17种,塔克拉玛干沙漠可能是一个新的beta- 内酰胺环抗生素耐药细菌的自然资源库.
英文摘要	During the surveying of indigenous bacterial diversity in Taklimakan Desert of Xinjiang of China, we obtained 10 isolates, which possess a capacity to grow on 0.1*Tryptic Soy Broth agar supplemented with 1000 mg/L ampicillin. These bacteria display extensive resistances to different beta-lactam antibiotics. All of these isolates belong to Proteobacteria. Five of them were identified as the human opportunistic pathogen Stenotrophomonas matltophila. Four strains were closely related to Mesorhizobium amorphae. It is interesting to see that one isolate A-3-E~T showed low 16S rRNA gene sequence similarity (< 96.8%) to those of the recognized species. The results of polyphasic taxonomy showed the strain represents a novel species of the new genus, for which the name Paramesorhizobium desertii gen. nov., sp. nov. is proposed. Moreover, it was found that high concentrations of beta-lactam antibiotics are not able to inhibit the growth of strain A-3-E~T. The novel bacterium grows well in the media containing 1000 mg/L of cefazolin or 250 mg/L of cefotaxime. The strain also resists 17 of the 28 tested antibiotics. We just presented a case that the Taklimakan Desert is a natural reservoir of novelbeta-Lactam antibiotic resistant bacteria.
中文关键词	beta - 内酰胺类抗生素 ; 抗药细菌 ; 塔克拉马干沙漠
英文关键词	beta-lactam antibiotics antibiotic resistant bacteria Taklimakan Desert
语种	英语
国家	中国
收录类别	CSCD
WOS类目	MICROBIOLOGY
WOS研究方向	Microbiology
CSCD记录号	CSCD:5334952
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/233260
作者单位	1.College of Resources and Environment, Huazhong Agricultural University, State Key Laboratory of Agricultural Microbiology, Wuhan, Hubei 430070, China; 2.School of Environmental Studies, China University of Geosciences (Wuhan), Wuhan, Hubei 430074, China; 3.College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China; 4.College of Bioengineering, Hubei University of Technology, Wuhan, Hubei 430068, China
推荐引用方式 GB/T 7714	罗雪松,潘云帆,石晶晶,等. 塔克拉马干沙漠存在超耐beta-内酰胺环抗生素微生物的证据及对一个新物种的生物化学特性描述[J],2015,42(1):56-64.
APA	罗雪松.,潘云帆.,石晶晶.,代俊.,侯莎.,...&彭芳.(2015).塔克拉马干沙漠存在超耐beta-内酰胺环抗生素微生物的证据及对一个新物种的生物化学特性描述.生物化学与生物物理进展,42(1),56-64.
MLA	罗雪松,et al."塔克拉马干沙漠存在超耐beta-内酰胺环抗生素微生物的证据及对一个新物种的生物化学特性描述".生物化学与生物物理进展 42.1(2015):56-64.