干旱区生态环境知识资源中心(Arid): UV-B辐射和干旱对丹参生长和叶片中酚酸类成分的影响

Arid

	UV-B辐射和干旱对丹参生长和叶片中酚酸类成分的影响
其他题名	The effects of drought and UV-B radiation on the growth and the phenolic compounds of the Salvia miltiorrhiza Bunge leaf
	刘景玲; 齐志鸿; 郝文芳; 张辰露; 刘峰华; 梁宗锁; 刘文婷
来源期刊	生态学报
ISSN	1000-0933
出版年	2015
卷号	35 期号:14 页码:4642-4650
中文摘要	在3种UV-B辐射强度以及3种干旱的单因子以及复合作用下,采用盆栽法对丹参植株生物量、叶面积、株高等形态指标和光合参数以及叶片中丹酚酸B、迷迭香酸、咖啡酸和丹参素含量的变化进行研究。旨在探索西北干旱、半干旱地区强光和干旱对丹参生物量和叶片中酚酸类含量的影响,为丹参的深入开发利用提供依据。结果表明:干旱和UV-B辐射单因子作用均对丹参生长指标有抑制作用,而对酚酸积累有促进作用;相比于干旱,UV-B辐射的影响更加显著。UV-B辐射显著引起植株矮化、叶面积减小、生物量降低、净光合速率降低以及叶片中酚酸类物质含量增加,两种因子复合作用对酚酸类物质积累的效果表现为复杂的加强或交互抗性作用。两种因子单独和复合作用处理下,适宜水分和重度UV-B辐射复合处理(75%T2)最有利于丹参叶片中酚酸类物质积累。
英文摘要	Ultraviolet B and drought stress are common environmental stresses in arid and semi-arid regions of northwestern China. A pot experiment was conducted to investigate the separate and combined effects of UV-B radiation and drought stress on physiological characteristics of Salvia miltiorrhiza Bunge in this region, and on the accumulation of phenolic compounds in its leaves. In this experiment, the UV-B radiation was performed with two intensities, 2.60 kJ/m~2(Tl) and 4.10 kj/m~2(T2), meanwhile, three gradual drought stress treatments were carried out with field water capacity ranging from 40% to 75%. The involved physiological characteristics included plant height, leaf area index, shoot biomass, root biomass,total biomass,root: shoot ratio, and those photosynthetic indices,such as net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and intercellular CO_2 concentration (Ci). The phenolic compounds detected in leaves contained salvianolic acid B (SAB),rosmarinic acid (RA),caffeic acid (CA) and 3, 4-trihydroxybenzenepropanoic acid (DSS). The results showed that both UV-B radiation and drought stress had cross-talk on the growth of S. miltiorrhiza, and the least plant height was found in the combined treatment (40%T2), which significantly decreased 47.82%. Drought stress and UV-B radiation reduced leaf area 40.22% and 71.51%,respectively, and the least value occurred in the combined treatment 75%T2. Similarly, the least root: shoot ratio also happened in combined treatment 75%T1 and the least total plant biomass did in 40%T2. In terms of the effects of UV-B and drought stress on photosynthetic indices, similar tendency was observed among Pn, Tr and Gs, but Ci had reverse trend with these three indices. The three indices were found reaching the largest at growth season,but decreased to the lowest at harvest period in the combined 40% T2 treatment. Both UV-B and drought stress could promote the accumulation of four phenolic compounds, in which the former had more prominent influence. Both contents of SAB and RA increased 4.28 and 8 folds, respectively, under UV-B radiation, which were significantly higher than 1.12 and 1.51 folds in drought stress treatment. The SAB and RA accumulation reached the highest level (4.75 and 3.15 mug/g, respectively) in the combined treatment 75%T2. Statistical data also exhibited that there was a cross-talk in accumulation of phenolic compounds between UV-B and drought stress. The SAB content increased 4.28 folds in T2 radiation intensity, while only 2.18 folds were observed in Tl. The content increased 1.12 and 0.52 folds in drought stress treatment with 40% and 55% field water capacity, respectively. If combined UV-B radiation and drought stress, the SAB content would increased 2.69 and 1.67 folds in 40%T2 and 55%T2 treatments, respectively. In conclusion, there was a cross-talk between UV-B radiation and drought stress, which had combined effects on the growth of S. miltiorrhiza and accumulation of phenolic compounds in leaves. Relatively, UV-B radiation had stronger influence than drought stress. Either UV-B radiation or drought stress had negative effect on plant growth. The higher UV-B radiation intensity (T2, 4.10 kj/m~2) was helpful to accumulation of SAB, but the amount of accumulation would be decreased with the increase of drought stress intensity.
中文关键词	丹参 ; 干旱 ; 形态 ; 丹酚酸B
英文关键词	UV-B Salvia miltiorrhiza Bunge UV-B drought morphology salvianolic acid B
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Life Sciences & Biomedicine - Other Topics
WOS研究方向	Life Sciences & Biomedicine - Other Topics
CSCD记录号	CSCD:5485874
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/233181
作者单位	刘景玲, 西北农林科技大学,生命学院, 杨凌, 712100.; 郝文芳, 西北农林科技大学,生命学院, 杨凌, 712100.; 梁宗锁, 西北农林科技大学,生命学院, 杨凌, 712100.; 刘文婷, 西北农林科技大学,生命学院, 杨凌, 712100.; 齐志鸿, 天津天士力现代中药资源公司, 天津 300402, 中国.; 刘峰华, 天津天士力现代中药资源公司, 天津 300402, 中国.; 张辰露, 陕西理工学院,生物科学与工程学院, 汉中, 陕西 723001, 中国.
推荐引用方式 GB/T 7714	刘景玲,齐志鸿,郝文芳,等. UV-B辐射和干旱对丹参生长和叶片中酚酸类成分的影响[J]. 西北农林科技大学,2015,35(14):4642-4650.
APA	刘景玲.,齐志鸿.,郝文芳.,张辰露.,刘峰华.,...&刘文婷.(2015).UV-B辐射和干旱对丹参生长和叶片中酚酸类成分的影响.生态学报,35(14),4642-4650.
MLA	刘景玲,et al."UV-B辐射和干旱对丹参生长和叶片中酚酸类成分的影响".生态学报 35.14(2015):4642-4650.