干旱区生态环境知识资源中心(Arid): 新疆杨不同成熟度叶片在碱胁迫下的生理响应

Arid

	新疆杨不同成熟度叶片在碱胁迫下的生理响应
其他题名	Physiological Responses of Populus alba var. pyramidalis Leaves with Different Maturity to Alkali Stress
	张选; 肖薪龙; 李鹏勃; 孙玉芳; 郭琬莹; 姚银安
来源期刊	林业科学
ISSN	1001-7488
出版年	2015
卷号	51 期号:12 页码:9-16
中文摘要	【目的】了解碱胁迫下不同成熟度杨树叶片的生理响应差异,为揭示干旱地区木本植物耐碱机制提供理论依据。【方法】以新疆地区分布面积较大的树种银白杨变种新疆杨为材料,进行碱处理(土壤pH8. 5 ~ 9. 0, 100 mmol·L~(-1) Na~+),分析碱胁迫下新疆杨不同成熟度叶片的离子含量(Na~+,K~+,Na~+/K~+,Mg~(2+),Ca~(2+))变化及分配规律、光和色素[叶绿素和类胡萝卜素(Car) ]响应和抗氧化生理指标[过氧化氢酶(CAT) 、超氧化物歧化酶(SOD) 、过氧化物酶(POD) 、丙二醛(MDA) ]的变化。【结果】与未成熟幼叶相比,对照条件下新疆杨老叶含有较高的Na~+ 含量和较低的K~+ 含量,有较高的Na~+/K~+; 碱胁迫下新疆杨的Na~+ 含量和Na~+/K~+ 在成熟老叶中进一步大幅度升高,而在幼叶中无明显变化,K~+ 含量在老叶和幼叶中受碱胁迫均无明显变化; 相反,二价离子(Ca~(2+)和 Mg~(2+))含量在对照条件下主要在老叶中积累,碱胁迫下Ca~(2+)和Mg~(2+)在成熟老叶中下降,而在幼叶中升高。新疆杨老叶的光合色素含量(叶绿素a,叶绿素b 和类胡萝卜素)在对照条件下显著高于幼叶,但是碱胁迫下老叶的光合色素含量大幅度下降并低于幼叶含量; 而幼叶的叶绿素(a,b 及总叶绿素)维持不变,类胡萝卜素甚至显著升高。同时,老叶膜脂过氧化水平(丙二醛含量)显著升高,在幼叶中仍然维持了较低水平。老叶内抗氧化酶活性如CAT、SOD、POD 碱胁迫下均大幅度升高,幼叶中上述抗氧化酶除SOD 酶稍微下降外均没有显著变化,保持较低水平。【结论】碱胁迫下新疆杨叶片多余的Na~+ 几乎完全进入了老叶,这种离子分配策略使老叶受到严重氧化胁迫; 面对盐碱敏感的幼叶可避免离子危害且受到良好的保护。这种有利于幼叶的离子分配方式是新疆杨抵抗碱胁迫的重要生存策略,对其在荒漠地区的生存具有重要意义。
英文摘要	【Objective】In this study,we investigated the effects of alkali stress on physiological characteristics of Populus alba var. pyramidalis leaves with different maturity,to provide a theoretical basis for indicating the strategy for woody plants to resist alkali stress in arid region. 【Method】We chose P. alba var. pyramidalis as the experimental material,and the species is a variant of P. alba and widely distributed in XinJiang area. The young P. alba var. pyramidalis plants were grown in soil pH8. 5 - 9. 0 and subjected to 100 mmol·L~(-1) Na~+. The change and distribution of ion content (Na~+,K~+,Na~+/K~+ ratio,Mg~(2+),Ca~(2+)),the response of photosynthetic pigment (Chl a,Chl b,Chl a + Chl b,Car) and the change of antioxidant physiological indices (CAT,SOD,POD,MDA) were investigated in young and old leaves of P. alba var. pyramidalis under alkali stress. 【Result】In the control condition,old leaves contained more Na~+ and less K~+ than young leaves,and hence a high Na~+/K~+ ratio in old leaves; The content of Na~+ and Na~+/K~+ ratio in old leaves increased significantly under alkali stress,but they had no obvious change in young leaves; The content of K~+ also had no significant change in young and old leaves under alkali stress; On the contrary,Mg~(2+) and Ca~(2+) were mainly accumulated in old leaves in control condition,but the content of them was decreased in old leaves and increased in young leaves under alkali stress. In control condition,the content of photosynthetic pigments (Chl a,Chl b,Car) was much higher in old leaves than in young leaves,but it declined significantly and was lower in old leaves than in young leaves under alkali stress; The content of Chl (Chl a,Chl b,Chl a + Chl b) in young leaves remained unchanged under alkali stress,but the content of Car in young leaves increased significantly under alkali stress. At the same time,the membrane lipid peroxide level (MDA content) in old leaves rose obviously under alkali stress,but it kept at control level in young leaves. The antioxidant enzymes activities (SOD,POD,CAT) in old leaves rose significantly,but they kept at control level in young leaves,except for that the activity of SOD declined.【Conclusion】Under alkali stress,the excessive Na~+ was mainly accumulated in old leaves of P. alba var. pyramidalis. The ion allocation strategy made old leaves suffer severe oxidative stress and thus protected young leaves that was salt-alkali sensitive from ion injure. Our study indicated that the ion allocation was in favor of young leaves,which was an important strategy for P. alba var. pyramidalis to resist alkali stress and was of great importance for its survival in desert region.
中文关键词	新疆杨 ; 碱胁迫 ; 幼叶 ; 老叶 ; 生理响应
英文关键词	Populus alba var. pyramidalis alkali stress young leaves old leaves physiological response
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Forestry
WOS研究方向	Forestry
CSCD记录号	CSCD:5605460
来源机构	中国科学院新疆生态与地理研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232992
作者单位	张选, 中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐, 新疆 830011, 中国.; 肖薪龙, 中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐, 新疆 830011, 中国.; 李鹏勃, 中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐, 新疆 830011, 中国.; 孙玉芳, 中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐, 新疆 830011, 中国.; 郭琬莹, 中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐, 新疆 830011, 中国.; 姚银安, 中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 乌鲁木齐, 新疆 830011, 中国.
推荐引用方式 GB/T 7714	张选,肖薪龙,李鹏勃,等. 新疆杨不同成熟度叶片在碱胁迫下的生理响应[J]. 中国科学院新疆生态与地理研究所,2015,51(12):9-16.
APA	张选,肖薪龙,李鹏勃,孙玉芳,郭琬莹,&姚银安.(2015).新疆杨不同成熟度叶片在碱胁迫下的生理响应.林业科学,51(12),9-16.
MLA	张选,et al."新疆杨不同成熟度叶片在碱胁迫下的生理响应".林业科学 51.12(2015):9-16.