干旱区生态环境知识资源中心(Arid): 超高效液相色谱法快速测定贝类中的软骨藻酸残留

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	超高效液相色谱法快速测定贝类中的软骨藻酸残留
其他题名	UHPLC Rapid Determination of Residual Domoic Acid in Shellfishes
	褚莹倩 1; 谢瀛 2; 陈溪 1; 崔晗 1; 张晓林 1; 黄大亮 1
来源期刊	理化检验. B, 化学分册
ISSN	1001-4020
出版年	2015
卷号	51 期号:10 页码:1396-1399
中文摘要	利用超高效液相色谱快速测定贝类中的软骨藻酸残留。样品经甲醇(1 + 1)溶液提取,采用Oasis MAX固相萃取柱对提取液进行净化后,在Waters ACQUITY UPLC BEH C_(18)色谱柱上分离,以乙腈-0.1%(体积分数)三氟乙酸(13+87)混合液为流动相进行洗脱,紫外检测波长为242 nm。软骨藻酸的线性范围为0.1~10.0 mg ?L~(-1), 测定下限(10S/N)为0.2 mg ? kg~(-1)。加标回收率在76.8%~91.2%之间,测定值的相对标准偏差(n=6)在11%~16%之间。
英文摘要	UHPLC was applied to the determination of residual domoic acid in shellfishes. The sample was extracted with methanol (1 + 1) solution. The extract was purified on Oasis MAX solid extraction column and the supernatant was separated on Waters ACQUITY UPLC BEH C_(18) chromatographic column with acetonitrile-0.1 % (phi) trifluoroacetic acid solution (13+87) as the mobile phase for isocratic elution. UV-detection was made at the wavelength of 242 nm. The linearity range of domoic acid was 0.1 10.0 mg ? L~(-1), with the lower limit of determination (10S/N) of 0.2 mg ? kg~(-1). Recovery rates obtained by standard addition method were in the range of 76.8%-91.2%, and RSDs (n=6) were in the range of 11%-16%.
中文关键词	超高效液相色谱法 ; 软骨藻酸 ; 贝类
英文关键词	UHPLC Domic acid Shellfishes
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL
WOS研究方向	Chemistry
CSCD记录号	CSCD:5547380
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232977
作者单位	1.大连出入境检验检疫局, 大连, 辽宁 116400, 中国; 2.本溪市疾病预防控制中心, 本溪, 辽宁 117000, 中国
推荐引用方式 GB/T 7714	褚莹倩,谢瀛,陈溪,等. 超高效液相色谱法快速测定贝类中的软骨藻酸残留[J],2015,51(10):1396-1399.
APA	褚莹倩,谢瀛,陈溪,崔晗,张晓林,&黄大亮.(2015).超高效液相色谱法快速测定贝类中的软骨藻酸残留.理化检验. B, 化学分册,51(10),1396-1399.
MLA	褚莹倩,et al."超高效液相色谱法快速测定贝类中的软骨藻酸残留".理化检验. B, 化学分册 51.10(2015):1396-1399.