干旱区生态环境知识资源中心(Arid): 新疆沙冬青AM真菌群落结构与遗传多样性分析

Arid

	新疆沙冬青AM真菌群落结构与遗传多样性分析
其他题名	Community composition and genetic diversity of arbuscular mycorrhizal fungi in the rhizosphere of Ammopiptanthus nanus in Xinjiang desert area
	张玉洁; 贺学礼; 程春泉; 赵金莉; 赵丽莉
来源期刊	菌物学报
ISSN	1672-6472
出版年	2015
卷号	34 期号:3 页码:375-385
中文摘要	以新疆乌恰县4个样地新疆沙冬青Ammopiptanthus nanus根围3个土层土壤为研究对象,利用Nested-PCR-DGGE技术,结合DGGE图谱分析、DNA克隆测序及系统发育分析,研究新疆沙冬青AM真菌菌群结构与遗传多样性特征,为新疆沙冬青根围功能菌群研究奠定基础。结果表明,不同样地和土层,AM真菌具有不同DGGE指纹图谱特征,AM真菌丰度、优势度及其多样性指数均有差异,其中上阿图什30-40cm土层AM真菌丰度和多样性指数最高,分别为20和3.48。DGGE条带测序和系统发育分析显示,全部序列归为2属14种,球囊霉属Glomus是其优势属,其中Uncultured Glomus(AB698612)和Uncultured Glomus(AB698616)普遍存在于4样地3个土层,Glomus sp.(FN429104)和Uncultured Glomus(KC797120)是上阿图什30-40cm土层特有种,Glomus sp.(EU332717),Rhizophagus intraradices(FR750206)和Uncultured Glomus(AB698616)是新疆沙冬青根围优势种。G. indicum和Rh. intraradices在形态学鉴定中未出现,说明分子生物学技术对真菌多样性研究起着补充和完善的作用。
英文摘要	Ammopiptanthus nanus is an excellent sand-fixation and endangered plant that can not only well stand up to desert environment, but also maintain the balance and stability of desert ecosystem. To elucidate the community composition and genetic diversity of AM fungi in the rhizosphere of A. nanus by PCR-DGGE and DNA cloning and sequencing, samples from four different sites in Wuqia, Xinjiang were collected in June 2012. Soil samples were taken at depth intervals of 10-20cm, 20-30cm and 30-40cm. The results showed that the fingerprint characteristics of DGGE, richness, dominance and diversity index of AM fungi varied with sampling sites and soil depth. Richness and diversity index of AM fungi were the highest at 30-40cm soil layer in Shang Artux, reaching 20 and 3.48 respectively. 20 AM fungal sequences coincide with 14 species in two genera, Glomus and Rhizophagus. G. indicum and Rh. intraradices were not recognized by morphological definition.
中文关键词	AM真菌丰度 ; 菌群结构 ; 遗传多样性 ; 新疆沙冬青
英文关键词	richness of AM fungi community structure genetic diversity Ammopiptanthus nanus
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FORESTRY
WOS研究方向	Forestry
CSCD记录号	CSCD:5431319
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232949
作者单位	河北大学生命科学学院, 保定, 河北 071002, 中国
推荐引用方式 GB/T 7714	张玉洁,贺学礼,程春泉,等. 新疆沙冬青AM真菌群落结构与遗传多样性分析[J],2015,34(3):375-385.
APA	张玉洁,贺学礼,程春泉,赵金莉,&赵丽莉.(2015).新疆沙冬青AM真菌群落结构与遗传多样性分析.菌物学报,34(3),375-385.
MLA	张玉洁,et al."新疆沙冬青AM真菌群落结构与遗传多样性分析".菌物学报 34.3(2015):375-385.