干旱区生态环境知识资源中心(Arid): 荒漠甲虫小胸鳖甲胞外信号调节激酶ERK基因的克隆与低温表达谱分析

Arid

	荒漠甲虫小胸鳖甲胞外信号调节激酶ERK基因的克隆与低温表达谱分析
其他题名	Cloning and expression analysis of extracellular signal-regulated kinase ERK gene from the desert beetle Microdera punctipennis
	孟闪闪; 李洁琼; 阮梦鸽; 陆雪莹; 马纪
来源期刊	环境昆虫学报
ISSN	1674-0858
出版年	2015
卷号	37 期号:5 页码:987-995
中文摘要	昆虫属变温生物,冷胁迫是其最常见的环境刺激。丝裂原活化蛋白激酶(MAPK)信号通路与细胞应对外界刺激密切相关,为了解MAPK信号通路在荒漠甲虫小胸鳖甲耐寒机制中的作用,从小胸鳖甲4℃转录组数据中筛选出胞外信号调节激酶(ERK)基因的全长cDNA,命名为MpERK。生物信息学分析表明,MpERK全长1125 bp,编码374个氨基酸。 MpERK的理论分子量是43 kDa,含有ERK激酶所共有的磷酸化位点,属于MAPK超家族,与其它物种ERK的一致性达80%以上。实时定量PCR检测MpERK基因在低温下的表达谱发现,4℃处理1 h后,MpERK基因的表达上调为对照的7.5倍,5 h时达到高峰,为对照的15倍。-4℃处理1 h后,MpERK基因的表达上调为对照的8.4倍,5 h时达到高峰,为对照的13.75倍。研究结果表明MpERK基因是冷响应的,可能在小胸鳖甲应对低温时发挥信号转导作用。
英文摘要	Cold stress is one of the most common environmental stimuli to insect. MAPK signaling pathway is closely related to cellular response to external stimuli. To study the function of MAPK signaling pathway in the cold hardy mechanism of the desert beetle Microdera punctipennis, a cDNA of an extracellular signal-regulated protein kinase (ERK) gene, designated as MpERK, was selected from the transcriptomic data of M. punctipennis that was treated for three hours at 4℃.Bioinformatic analysis showed that the full length of MpERK was 1125 bp, encoding 374 amino acids. The predicted molecular mass of MpERK was 43 kDa, containing the highly conserved kinase phosphorylation site. MpERK belonged to MAPK superfamily, and shared over 80% identity with that of other species. Real-time quantitative PCR analysis showed that after treated the insect was at 4℃ for 1 h, the expression of MpERK was upregulated as 7.5 fold of the control, and 15 fold of the control after 5 h treatment. The expression of MpERK was also induced by -4℃ in that it was 8.4 fold and 13.75 fold of the control after 1 h and 5 h treatment respectively. Our results indicated that MpERK is cold responsive, and may play role in signal transduction in M. punctipennis when it is exposed to low temperatures.
中文关键词	MAPK通路 ; 胞外信号调节激酶 ; 小胸鳖甲 ; 低温 ; ERK信号通路
英文关键词	MAPK pathway extracellular signal-regulated protein kinase Microdera punctipennis low temperature ERK signaling pathway
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENTOMOLOGY
WOS研究方向	Entomology
CSCD记录号	CSCD:5574775
来源机构	新疆大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232933
作者单位	新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 乌鲁木齐, 新疆 830046, 中国
推荐引用方式 GB/T 7714	孟闪闪,李洁琼,阮梦鸽,等. 荒漠甲虫小胸鳖甲胞外信号调节激酶ERK基因的克隆与低温表达谱分析[J]. 新疆大学,2015,37(5):987-995.
APA	孟闪闪,李洁琼,阮梦鸽,陆雪莹,&马纪.(2015).荒漠甲虫小胸鳖甲胞外信号调节激酶ERK基因的克隆与低温表达谱分析.环境昆虫学报,37(5),987-995.
MLA	孟闪闪,et al."荒漠甲虫小胸鳖甲胞外信号调节激酶ERK基因的克隆与低温表达谱分析".环境昆虫学报 37.5(2015):987-995.