干旱区生态环境知识资源中心(Arid): 营养盐与盐双重胁迫下水体中浮游细菌的响应

Arid

	营养盐与盐双重胁迫下水体中浮游细菌的响应
其他题名	Response of Freshwater Bacterial Community to the Double Stress of Salinity and Nutrients
	张磊; 高光; 李锐; 许秋瑾; 蔡华珍
来源期刊	环境科学研究
ISSN	1001-6929
出版年	2015
卷号	28 期号:12 页码:1843-1851
中文摘要	为阐释干旱地区内陆湖泊微生物群落对营养盐和盐双重胁迫的响应机制,针对干旱、半干旱地区许多湖泊水体受咸化与富营养化双重影响的特点,通过454高通量测序16S rRNA基因和多元统计分析,研究水体营养盐和盐的添加对细菌群落结构、多样性变化的影响.结果表明,盐与营养盐的输入引起了水体中细菌群落组成和多样性的显著变化,具体表现为: ①随着盐度(以w计)的升高,alpha-变形菌纲(Alphaproteobacteria)的相对丰度显著增加,从对照处理的16.0% 4.6%增至SO(贫营养-盐水)处理的39.8% 18.1%;但会受到水体营养水平的制约. ②富营养水平下,微咸条件(盐度为1.5)有利于细菌类群丰富度的增加,BE-30(微咸-富营养处理培养30 d样品)的Shannon-Wiener多样性指数(4.62)显著高于FE-30(淡水-富营养处理培养30 d样品, 2.08)和SE-30(高盐-富营养处理培养30 d样品, 1.69); ③高盐(盐度为3.0)条件下,随着水体中营养盐含量的增加,喜营养的嗜盐细菌类群大量繁殖(SE-30中无法分类的Comamonadaceae科的相对丰度达到69.3%), 因而降低了水体中细菌群落的多样性.研究显示,alpha-变形菌纲和无法分类的Comamonadaceae科对盐与营养盐的变化较为敏感,可作为干旱区湖泊咸化和富营养化过程的参考类群.
英文摘要	To characterize the dual impacts of salinization and eutrophication in aquatic ecosystems in arid regions,16S rRNA gene based high-throughput sequencing and multivariate statistical analysis were employed to investigated the changes of bacterial communities and diversity in freshwater of inland arid regions in response to a manipulated supply of salinity and inorganic nutrients. The results showed that the addition of salinity and nutrients induced a clear shift in the bacterial communities and diversity. Detailed analysis results showed that: 1) Alphaproteobacteria increased from 16.0% 4.6% in the control (C) group to 39.8% 18.1% in the saline-oligotrophic (SO) group with increased salinity,although it might be restrained by different nutrient levels. 2) Brackish habitats (1.5) facilitated enhanced bacterial diversity in the eutrophic water. For example,the Shannon-Wiener diversity index of sample BE-30 (4.62) was significantly higher than that of samples FE-30 (2.08) and SE-30 (1.69). 3) Nutrient-loving Betaproteobacteria (especially 69.3% unclassified Comamonadaceae in sample SE-30) showed fast growth in response to elevated nutrient concentrations,but the bacterial diversity in the saline habitats decreased significantly with the increased nutrients. Alphaproteobacteria and unclassified Comamonadaceae showed their sensitivities to the change in salinity and nutrients,and they might be used as indicator bacterial taxa to identify the salinization and eutrophication process in lakes in arid regions.
中文关键词	细菌群落 ; 高通量测序 ; 营养盐 ; 盐 ; 干旱区湖泊
英文关键词	bacterial community high-throughput sequencing nutrient salinity lakes in arid regions
类型	Article
语种	中文
国家	中国
收录类别	CSCD
WOS类目	Environmental Sciences & Ecology
WOS研究方向	Environmental Sciences & Ecology
CSCD记录号	CSCD:5600181
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232923
作者单位	张磊, 滁州学院生物与食品工程学院, 滁州, 安徽 239000, 中国.; 蔡华珍, 滁州学院生物与食品工程学院, 滁州, 安徽 239000, 中国.; 高光, 中国科学院南京地理与湖泊研究所, 湖泊与环境国家重点实验室, 南京, 江苏 210008, 中国.; 李锐, 中国环境科学研究院, 环境基准与风险评估国家重点实验室, 北京 100012, 中国.; 许秋瑾, 中国环境科学研究院, 环境基准与风险评估国家重点实验室, 北京 100012, 中国.
推荐引用方式 GB/T 7714	张磊,高光,李锐,等. 营养盐与盐双重胁迫下水体中浮游细菌的响应[J],2015,28(12):1843-1851.
APA	张磊,高光,李锐,许秋瑾,&蔡华珍.(2015).营养盐与盐双重胁迫下水体中浮游细菌的响应.环境科学研究,28(12),1843-1851.
MLA	张磊,et al."营养盐与盐双重胁迫下水体中浮游细菌的响应".环境科学研究 28.12(2015):1843-1851.