干旱区生态环境知识资源中心(Arid): 不同轮作制下长期施肥旱地土壤微生物多样性特征

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	不同轮作制下长期施肥旱地土壤微生物多样性特征
其他题名	Effect of Long-term Fertilization on Microbial Diversity of Black Loessial Soil Based on 454 Sequencing Technology
	蔡艳 1; 郝明德 2; 臧逸飞 2; 何晓雁 2; 张丽琼 2
来源期刊	核农学报
ISSN	1000-8551
出版年	2015
卷号	29 期号:2 页码:344-350
中文摘要	为了探讨典型旱地区施肥制度对土壤微生物多样性的影响,本研究通过27年长期定位试验,采用454测序技术,分析了黄土旱地区小麦连作、苜蓿连作、粮豆轮作中不同化肥及有机肥施肥措施下土壤细菌、真菌多样性的变化情况。结果表明:除施氮肥和有机肥(NM)处理外,施肥后连作小麦土壤细菌Chao指数提高7.5% (施磷肥和有机肥,PM)~ 60.6% (单施有机肥,M),Shannon指数增加0.04 (PM)~ 0.40(M),肥料单施优于配施;施肥后连作苜蓿土壤细菌Chao指数提高92.0% (单施磷肥,P)~ 102.4%(施氮肥、磷肥和有机肥,NPM),Shannon指数增加0.30(NPM)~ 0.32(P);施肥对粮豆轮作土壤细菌多样性影响较小。除小麦连作NP处理外,施肥后各种植系统土壤真菌Chao指数提高19.1% ~ 55.0%,但真菌Shannon指数变化不大或明显下降。黄土旱地区作物连作时施与适当化肥或有机肥,可提高土壤细菌多样性。本研究对优化旱地作物施肥制度、发挥土壤潜力肥力、实现土壤资源可持续利用有着重要的意义。
英文摘要	In order to the influence of cropping systems on soil microbial diversity in typical arid areas.the paper analyzed the changes of diversity of bacteria and fungi from black loessial after fertilized by chemical fertilizer or organic fertilizer under continuous planting wheat,continuous planting alfalfa and wheat-pea rotation based on 454 sequencing technology in Loess Plateau According to 27-year long-term field experiment,The results showed that: Except NM (application of N fertilizer and organic manure) treatment,Chao indexes and Shannon indexes in soil of continuous wheat increased by 7.5% (PM,meaning application of P fertilizer and organic manure) to 60.6% (M,meaning application of organic manure) and 0.04 (PM) to 0.40 (M) respectively after fertilization,and fertilizer administered alone was more obvious than fertilizer mixed other fertilizer.Chao indexes of bacteria in soil of continuous alfalfa increased by 92.0% (P,meaning application of P fertilizer) to 102.4% (NPM,meaning application of nitrogen fertilizer,phosphate fertilizer and organic manure) after fertilization,and Shannon indexes of those increased by 0.30 (NPM) to 0.32 (P).Fertilization had small impact on soil bacteria diversity of wheat-pea rotation.Chao indexes of fungi in soil of various cropping systems increased by 19.1% to 55.0% after fertilization except continuous wheat fertilized nitrogen fertilizer and phosphate fertilizer,but Shannon indexes of fungi changed little or decreased significantly.In Loess dryland areas,It could improve the bacterial diversity significantly that applying proper chemical fertilizer or manure fertilizer when continous planting single crop.It has important significance on optimizing dryland cropping systems,playing the soil potential fertility and achieving the sustainable use of soil resources.
中文关键词	454测序 ; 旱地土壤 ; 微生物多样性 ; 长期施肥
英文关键词	the 454 sequencing dry land soil microbial diversity long-term fertilization
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:5358763
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232886
作者单位	1.四川农业大学资源环境学院, 成都, 四川 611130, 中国; 2.西北农林科技大学资源环境学院, 杨凌, 陕西 712100, 中国
推荐引用方式 GB/T 7714	蔡艳,郝明德,臧逸飞,等. 不同轮作制下长期施肥旱地土壤微生物多样性特征[J]. 西北农林科技大学,2015,29(2):344-350.
APA	蔡艳,郝明德,臧逸飞,何晓雁,&张丽琼.(2015).不同轮作制下长期施肥旱地土壤微生物多样性特征.核农学报,29(2),344-350.
MLA	蔡艳,et al."不同轮作制下长期施肥旱地土壤微生物多样性特征".核农学报 29.2(2015):344-350.