干旱区生态环境知识资源中心(Arid): 胶孢炭疽菌拮抗菌株的筛选、鉴定及抑菌物质分析

Arid

	胶孢炭疽菌拮抗菌株的筛选、鉴定及抑菌物质分析
其他题名	Screening, Identification and Bacteriostat Qualitative Analysis of Antagonistic Strain against Colletotrichum gloeosporioides
	吕纪涛 1; 魏莉 2; 曹晓璐 3; 杨继业 1; 郭晓军 1; 李潞滨 3; 尹艳军 1
来源期刊	河南农业科学
ISSN	1004-3268
出版年	2015
卷号	44 期号:7 页码:89-93
中文摘要	为了筛选获得高效拮抗建兰炭疽病病原菌胶孢炭疽菌(Colletotrichum gloeosporioides )的菌株,采用平板对峙法,从新疆库姆塔格沙漠土样分离的126株细菌中初筛获得23株拮抗菌株,经平板扩散法复筛得到1株高效拮抗菌株N147 -1 -3。结合菌落菌体形态观察、16S rDNA序列分析和生理生化试验,鉴定该茵为甲基营养型芽孢杆菌(Bacillus methylotrophicus)。对拮抗菌发酵液上清测定表明,60%的硫酸铵沉淀物具有最高的抑菌活性,经氯仿抽提后萃取相不具有拮抗活性,经高温处理和蛋白酶K消化后抑菌物质的拮抗作用消失,初步分析抑菌物质是蛋白类物质。
英文摘要	Twenty-three strains were obtained by screening strains with high antagonism against Colletotrichum gloeosporioides by the tablet confrontation method from 126 strains,which were isolated from Xinjiang Kumtag desert samples. Strain N147-1 -3 with highest antagonism was obtained by using the plate diffusion method. Combined with the morphology observation of colonies and cells, 16S rDNA sequence analysis,physiological and biochemical characteristics tests,the strain was finally identified as Bacillus methylotrophicus. The test results of the supernatant of the strain fermented liquid showed that the sediment by adding 60% ammonium sulfate had the highest antibacterial activity. The supernatant of the strain fermented liquid lost its antimicrobial activity after the chloroform extraction. The antagonism effect of the strain disappeared after treatments by high temperatures or proteinase K. The antibacterial substance was finally identified as protein.
中文关键词	建立炭疽病 ; 胶孢炭疽菌 ; 芽孢杆菌 ; 拮抗作用 ; 筛选 ; 鉴定 ; 抑菌物质
英文关键词	cymbidium anthracnose Colletotrichum gloeosporioides Bacillus antagonism screening identification antibacterial substance
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:5468164
来源机构	中国林业科学研究院林业研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232881
作者单位	1.河北农业大学生命科学学院, 保定, 河北 071000, 中国; 2.邯郸市环境监测中心站, 邯郸, 河北 056002, 中国; 3.中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 北京 100091, 中国
推荐引用方式 GB/T 7714	吕纪涛,魏莉,曹晓璐,等. 胶孢炭疽菌拮抗菌株的筛选、鉴定及抑菌物质分析[J]. 中国林业科学研究院林业研究所,2015,44(7):89-93.
APA	吕纪涛.,魏莉.,曹晓璐.,杨继业.,郭晓军.,...&尹艳军.(2015).胶孢炭疽菌拮抗菌株的筛选、鉴定及抑菌物质分析.河南农业科学,44(7),89-93.
MLA	吕纪涛,et al."胶孢炭疽菌拮抗菌株的筛选、鉴定及抑菌物质分析".河南农业科学 44.7(2015):89-93.