干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱法测定不同茶叶中草甘膦、氨甲基膦酸及草铵膦的残留

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	超高效液相色谱-串联质谱法测定不同茶叶中草甘膦、氨甲基膦酸及草铵膦的残留
其他题名	Determination of Glyphosate, Aminomethyl Phosphonic Acid and Glufosinate in Different Teas by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
	诸力; 陈红平; 周苏娟; 王川丕; 刘新
来源期刊	分析化学
ISSN	0253-3820
出版年	2015
卷号	43 期号:2 页码:271-276
中文摘要	建立了超高效液相色谱-串联质谱同时快速测定不同茶叶中草甘膦、氨甲基膦酸及草铵膦的方法。样品用0.05 mol/L NaOH提取, 并以HCl调节pH值, Oasis HLB小柱净化除杂, 氯甲酸-9-芴基甲酯(FMOC-Cl)柱前衍生反应后, 超高效液相色谱-串联质谱法测定。本方法在5~1000 mug/L浓度范围内, 不同茶叶基质中草甘膦、氨甲基膦酸、草铵膦线性关系良好(R~2>0.99)。在0.1, 0.4和4 mg/kg添加水平下, 不同茶叶(绿茶、红茶、乌龙茶、普洱茶)中3种化合物回收率均介于72.1%~109.9%之间, 相对标准偏差RSD在0.5%~9.8%之间(n=6), 方法定量限(LOQ)在0.03~0.08 mg/kg之间(S/N=10)。本方法稳定, 简便, 灵敏, 能够满足检测需求。
英文摘要	An efficient technique for the determination of multiclass pesticide residues (glyphosate, aminomethyl phosphonic acid (AMPA) and glufosinate) in four kinds of tea (green tea, black tea, Oolong tea and Puer tea) was developed based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were extracted with 0.05 mol/L NaOH solution, regulated pH value with HCl, followed by purification by Oasis HLB column, and then precolumn-derived with 9-fluorenylmethyl chloroformate (FMOC-CL) in borate buffer. All pesticide residues studied showed good linearity with correlation coefficient (R~2) greater than 0.99 in the concentration range of 5-1000 mug/L. Limits of quantitation (LOQs) of three pesticide compounds ranged from 0.03 to 0.08 mg/kg (S/N=10). Tea samples spiked with 0.1, 0.4 and 4 mg/kg showed the recovery ranging from 72.1% to 109.9%, and RSDs from 0.5% to 9.8% (n=6). In addition, the validated method was applied to commercial samples, and all the detections were confirmed by acquiring transitions for each pesticide in samples.
中文关键词	茶叶 ; 草甘膦 ; 氨甲基膦酸 ; 草铵膦 ; 超高效液相色谱-串联质谱
英文关键词	Tea Glyphosate Aminomethyl phosphonic acid Glufosinate Ultra performance liquid chromatography-tandem mass spectrometry
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FOOD SCIENCE TECHNOLOGY
WOS研究方向	Food Science & Technology
CSCD记录号	CSCD:5363209
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232582
作者单位	中国农业科学院茶叶研究所, 农业部茶叶产品质量安全风险评估实验室, 杭州, 浙江 310008, 中国
推荐引用方式 GB/T 7714	诸力,陈红平,周苏娟,等. 超高效液相色谱-串联质谱法测定不同茶叶中草甘膦、氨甲基膦酸及草铵膦的残留[J],2015,43(2):271-276.
APA	诸力,陈红平,周苏娟,王川丕,&刘新.(2015).超高效液相色谱-串联质谱法测定不同茶叶中草甘膦、氨甲基膦酸及草铵膦的残留.分析化学,43(2),271-276.
MLA	诸力,et al."超高效液相色谱-串联质谱法测定不同茶叶中草甘膦、氨甲基膦酸及草铵膦的残留".分析化学 43.2(2015):271-276.