干旱区生态环境知识资源中心(Arid): 超高效液相色谱-串联质谱法同时测定祛痘化妆品中的15种禁用雄激素

Arid

	超高效液相色谱-串联质谱法同时测定祛痘化妆品中的15种禁用雄激素
其他题名	Simultaneous Determination of 15 Prohibited Androgens in Anti-acne Cosmetics by Ultra Performance Liquid Chromatography - Tandem Mass Spectrometry
	李晶瑞; 马强; 孟宪双; 陈云霞; 马会娟; 张庆; 白桦
来源期刊	分析测试学报
ISSN	1004-4957
出版年	2015
卷号	34 期号:1 页码:43-49
中文摘要	建立了同时测定祛痘化妆品中15种禁用雄激素(肾上腺甾酮、群勃龙、勃地酮、氟甲睾酮、氧甲氢龙、雄烯二酮、诺龙、去氢甲睾酮、睾酮、去氢表雄酮、氯司替勃、雄诺龙、美雄诺龙、醋酸氯睾酮、丙酸睾丸素)的超高效液相色谱-串联质谱方法。不同类型的化妆品样品,以甲醇为提取溶剂进行超声提取,样品提取液经离心处理后,上清液以Oasis HLB固相萃取柱净化,经ACQUITY UPLC BEH C_(18)(2.1mm*50mm, 1.7 mum)色谱柱分离,目标化合物在正离子模式下电离,采用多反应监测模式进行定性、定量分析。结果表明,15种禁用雄激素的定量下限为1.0~20.0 mug/kg,在低、中、高3个加标水平下的平均回收率为76.5%~92.1%,相对标准偏差为1.3%~9.6%。本方法准确、快速、灵敏,能够为祛痘化妆品检验和生产质量控制提供科学依据及技术支持。
英文摘要	A comprehensive analytical method based on ultra performance liquid chromatography tandem mass spectrometry was developed for the simultaneous determination of fifteen prohibited androgens (e. g. adrenosterone,trenbolone, boldenone,fluoxymesterone, oxandrolone, androstenedione,nandrolone, metandienone, testosterone, dehydroepiandrosterone, clostebol, stanolone, mestanolone, 4-chlorotestosterone acetate and testosterone propionate). Various cosmetic samples were extracted with methanol by ultrasonication. The extract was centrifuged, and the upper solution was then cleaned up with an Oasis HLB solid phase extraction cartridge. After chromatographic separation on an ACQUITY UPLC BEH C _(18)(2. 1 mm *50 mm, 1. 7 mum) column, the target compounds were analyzed under the multiple reaction monitoring (MRM) mode in positive ionization mode. The limits of quantitation (LOQs) for fifteen androgens were in the range of 1. 0 -20. 0 mug/kg. The mean recoveries at three spiked levels ranged from 76. 5% to 92. 1%,with relative standard deviations of 1.3% -9. 6%. The method was proved to be accurate,rapid and sensitive, and could provide a scientific basis and technical support for the inspection and production quality control of anti-acne cosmetics.
中文关键词	祛痘化妆品 ; 雄激素 ; 超高效液相色谱-串联质谱 ; 固相萃取
英文关键词	anti-acne cosmetics androgens ultra performance liquid chromatography - tandem mass spectrometry(UPLC - MS/MS) solid phase extraction
语种	中文
国家	中国
收录类别	CSCD
WOS类目	CHEMISTRY ANALYTICAL ; CHEMISTRY ORGANIC
WOS研究方向	Chemistry
CSCD记录号	CSCD:5338721
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232580
作者单位	中国检验检疫科学研究院, 北京 100123, 中国
推荐引用方式 GB/T 7714	李晶瑞,马强,孟宪双,等. 超高效液相色谱-串联质谱法同时测定祛痘化妆品中的15种禁用雄激素[J],2015,34(1):43-49.
APA	李晶瑞.,马强.,孟宪双.,陈云霞.,马会娟.,...&白桦.(2015).超高效液相色谱-串联质谱法同时测定祛痘化妆品中的15种禁用雄激素.分析测试学报,34(1),43-49.
MLA	李晶瑞,et al."超高效液相色谱-串联质谱法同时测定祛痘化妆品中的15种禁用雄激素".分析测试学报 34.1(2015):43-49.