干旱区生态环境知识资源中心(Arid): 气相色谱-质谱法初筛和确证运动营养品中甲基己胺

Arid

	气相色谱-质谱法初筛和确证运动营养品中甲基己胺
其他题名	Screening and Confirmation of Methylhexanamine in Sports Supplements through Gas Chromatography-Mass Spectrometry
	王占良; 张建丽; 张亦农
来源期刊	中国运动医学杂志
ISSN	1000-6710
出版年	2014
卷号	33 期号:10 页码:1004-1008
中文摘要	目的:建立气相色谱-质谱法初筛和确证运动营养品中甲基己胺快速检测方法。方法:样品采用甲醇超声提取,弱阳离子交换固相萃取柱(MCX)纯化,洗脱液再经过液液萃取提取,30℃下氮气吹干,N-甲基-N-(三甲基硅烷)三氟乙酰胺衍生化后,采用HP-1MS柱(17 m * 0.2 mm i.d. * 0.11 mm)色谱分离,程序升温,质谱检测。结果:甲基己胺的差向异构体在确证分析中实现基线分离,3个定性特征离子的峰度比计算结果满足世界反兴奋剂机构(WADA)要求。该方法的检测限为100 mug/kg,在添加的高、中、低3种浓度的相对回收率分布在75%~120%,满足常规检测要求,样品前处理简单、快速、可靠。
英文摘要	Objective To screen and confirm methylhexanamine in sports supplements through gas chromatography -mass spectrometry. Methods The sample was extracted by methanol and purified by MCX Oasis solid prepared extraction column, the elution was extracted by methyl tert -butyl ether, the methylhexanamine was completely separated by HP-1MS column (17 m * 0.2 mm i.d. x 0.11 mum thickness)in linear temperature program. Results The epimers of methylhexanamine was separated in baseline and the calculated results of three characteristic ions in methylhexanamine accorded to the requirement of WADA technical documents. The relative recovery of methylhexanamine spiked in blank sample at high, middle and low level concentration ranged from 75 to 120%. The limit of detection for target analytes was 100 mug/kg. Conclusion The method introduced here is simple,rapid,reliable and suitable for analysis of Moutine in sports supplements.
中文关键词	甲基己胺 ; 兴奋剂 ; 运动营养品 ; 气相色谱-质谱法(GC/MS)
英文关键词	methylhexanamine doping gas chromatography-mass spectrometry
语种	中文
国家	中国
收录类别	CSCD
WOS类目	MEDICINE GENERAL INTERNAL
WOS研究方向	General & Internal Medicine
CSCD记录号	CSCD:5351656
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232280
作者单位	国家体育总局反兴奋剂中心食品药品兴奋剂检测实验室, 北京 100029, 中国
推荐引用方式 GB/T 7714	王占良,张建丽,张亦农. 气相色谱-质谱法初筛和确证运动营养品中甲基己胺[J],2014,33(10):1004-1008.
APA	王占良,张建丽,&张亦农.(2014).气相色谱-质谱法初筛和确证运动营养品中甲基己胺.中国运动医学杂志,33(10),1004-1008.
MLA	王占良,et al."气相色谱-质谱法初筛和确证运动营养品中甲基己胺".中国运动医学杂志 33.10(2014):1004-1008.