干旱区生态环境知识资源中心(Arid): 液相色谱-串联质谱法检测鸡蛋中金刚烷胺残留

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	液相色谱-串联质谱法检测鸡蛋中金刚烷胺残留
其他题名	Determination of Amantadine in Fresh Eggs by LC-MS/MS
	汤晓艳; 陈东宇; 周剑; 毛雪飞; 邱静; 王敏
来源期刊	中国食品学报
ISSN	1009-7848
出版年	2014
卷号	14 期号:9 页码:190-194
中文摘要	采用液相色谱-串联质谱(LC-MS/MS)法检测鸡蛋中金刚烷胺，比较不同类型色谱柱、提取溶液（甲醇1%三氯乙酸水溶液与甲醇/氨-氯化铵缓冲液）和净化方式（正己烷分配去脂、正己烷/二氯甲烷反萃取及Oasis MCX固相萃取柱净化）。结果显示，用甲醇-1%三氯乙酸水溶液提取，正己烷分配去脂，经Agilent XDB-C18 (150mm*2.1 mm,3.5 m)色谱柱分离，LC-MS/MS检测效果最好。本方法检出限(L0D)为2 mug/kg，线性范围在1~ 1 000 mug/L之间，回收率88.0%~99.6%。方法操作简便，实用性强，精密度好，为禽产品的质量安全检测与监管提供方法依据。
英文摘要	An analytical method was developed to determine amantadine in fresh eggs by LC-MS/MS. Different chromatographic column, two extraction systems, which were methanol: 1% trichloroacetic acid aqueous solution and methanol / ammonia-ammonium chloride buffer, and three purification systems, which were assigned fat by N-hexane, N-hexane / dichloromethane anti-extraction and Oasis MCX solid phase extraction column purification were considered. The optimum condition was achieved by extracted with a mixed solvent of methanol-1% trichloroacetic acid, removing lipids with Nhexane, and separating by Agilent XDB-C18 (150 mmx2.1 mm,3.5 m). The linear range wasl~l 000 mug/kg, and the limit of detection was 2 mug/kg. The recoveries ranged from 88.0%~99.6%. The method provides scientific basis for the supervision of poultry eggs safety with simplified operation, good practicability and higher sensitivity.
中文关键词	金刚烷胺 ; 鸡蛋 ; 方法改进
英文关键词	LC-MS/MS LC-MS/MS amantadine fresh egg improved method
语种	中文
国家	中国
收录类别	CSCD
WOS类目	ENGINEERING MANUFACTURING
WOS研究方向	Engineering
CSCD记录号	CSCD:5248079
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232254
作者单位	中国农业科学院农业质量标准与检测技术研究所, 农业部农产品质量安全重点实验室, 北京 100081, 中国
推荐引用方式 GB/T 7714	汤晓艳,陈东宇,周剑,等. 液相色谱-串联质谱法检测鸡蛋中金刚烷胺残留[J],2014,14(9):190-194.
APA	汤晓艳,陈东宇,周剑,毛雪飞,邱静,&王敏.(2014).液相色谱-串联质谱法检测鸡蛋中金刚烷胺残留.中国食品学报,14(9),190-194.
MLA	汤晓艳,et al."液相色谱-串联质谱法检测鸡蛋中金刚烷胺残留".中国食品学报 14.9(2014):190-194.