干旱区生态环境知识资源中心(Arid): 红砂(Reaumuria soongorica)黄酮类物质代谢及其抗氧化活性对UV-B辐射的响应

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	红砂(Reaumuria soongorica)黄酮类物质代谢及其抗氧化活性对UV-B辐射的响应
其他题名	Responses of the Flavonoid Pathway and Antioxidant Ability to UV-B Radiation Stress in Reaumuria soongorica
	刘美玲; 曹波; 刘玉冰; 谭会娟; 陈永乐; 张亚峰
来源期刊	中国沙漠
ISSN	1000-694X
出版年	2014
卷号	34 期号:2 页码:426-432
中文摘要	对荒漠植物红砂(Reaumuria soongorica)在UV-B辐射胁迫下不同时间内(0、2、4、6、8、10d)脂质过氧化、叶绿素和类胡萝卜素含量的变化以及黄酮类代谢途径关键酶活性、代谢产物及代谢产物的抗氧化活性进行了分析,探讨黄酮类代谢响应UV-B辐射胁迫的变化以及与脂质过氧化和光合色素系统的相关性。结果表明:随着UV-B辐射处理时间的延长,叶绿素含量有所降低;类胡萝卜素含量升高;膜脂过氧化程度(以丙二醛(MDA)含量表示)增加;黄酮类代谢途径关键酶黄烷酮3-羟化酶(F3H)活性呈现先增加后降低的趋势,但仍高于处理前水平,二氢黄酮醇4-还原酶(DFR)活性增强,代谢终产物总黄酮含量先降低后恢复至处理前水平,花色素苷(ANS)含量上升;DFR活性、黄酮类代谢产物ANS及总黄酮对DPPH清除率和对MDA形成的抑制率均与MDA以及光合色素系统之间存在显著或极显著的相关性。这表明次生物质黄酮类代谢途径在UV-B胁迫下发挥了抗氧化功能,提高红砂对在 UV-B辐射下的自我保护能力。
英文摘要	Reaumuria soongorica is a dwarf shrub mainly found in desert areas of North-west China,and can survive severe environmental stress.Due to the depletion of ozone layer,enhanced UV-B radiation is becoming one most important component of terrestrial radiation that plants are exposed to.This study aimed to assess the responses of the flavonoid pathway and its antioxidant activity to UV-B radiation treatments and its correlation to the lipid peroxide and photosynthetic pigments in R.soongorica leaves.For UV-B treatment, the plants were planted under enhanced UV-B radiation with an intensity of 0.3 mW·cm~(-2)(20%higher than average natural UV-B radiation dose)at middy by hanging UV-B lamps above the plants.Contents of chlorophyll and carotenoid,levels of lipid peroxidation,activities of flavanone 3-hydroxylase (F3H )and dihydroflavonol 4-reductase(DFR),accumulations of total flavonoids and anthocyanins(ANS)and the scavenging 1,1-Diphenyl-2-picrylhydrazyl radical 2,2 diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl(DPPH)ability and malondialdehyde (MDA)inhibition rate of total flavonoids under different UV-B radiation treatments were investigated.The correlations between key enzymes,products and antioxidant ability of flavonoids in the flavonoid pathway and the lipid peroxide and photosynthetic pigments were also analyzed.The results showed that:The chlorophyll contents decreased and carotenoid content significantly increased with the treatment time increased.The level of MDA increased.The contents of total flavonoids decreased within 4 days and then recovered to the same level as untreatmented.The contents of ANS,DFR activity and ability of scavenging DPPH increased under UV-B stress.The activities of F3H increased within6 days and then decreased. The flavonoid products ANS,DFR activity and ability of scavenging DPPH and MDA inhibition had significant correlations with MDA level,as well as the photosynthetic pigments contents.Thus,we speculate that under enhanced UV-B radiation,the the flavonoid pathway played important role as antioxidant, and protected the R.soongoricafrom damaging by UV-B radiation stress.
中文关键词	抗氧化系统 ; 黄酮类 ; 红砂(Reaumuria soongorica) ; UV-B辐射胁迫
英文关键词	antioxidant enzyme system flavonoids Reaumuria soongorica UV-B radiation stress
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PLANT SCIENCES
WOS研究方向	Plant Sciences
CSCD记录号	CSCD:5102083
来源机构	中国科学院西北生态环境资源研究院
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232167
作者单位	中国科学院寒区旱区环境与工程研究所, 甘肃省寒区旱区逆境生理与生态重点实验室;;中国科学院沙坡头沙漠试验研究站, 兰州, 甘肃 730000, 中国
推荐引用方式 GB/T 7714	刘美玲,曹波,刘玉冰,等. 红砂(Reaumuria soongorica)黄酮类物质代谢及其抗氧化活性对UV-B辐射的响应[J]. 中国科学院西北生态环境资源研究院,2014,34(2):426-432.
APA	刘美玲,曹波,刘玉冰,谭会娟,陈永乐,&张亚峰.(2014).红砂(Reaumuria soongorica)黄酮类物质代谢及其抗氧化活性对UV-B辐射的响应.中国沙漠,34(2),426-432.
MLA	刘美玲,et al."红砂(Reaumuria soongorica)黄酮类物质代谢及其抗氧化活性对UV-B辐射的响应".中国沙漠 34.2(2014):426-432.