干旱区生态环境知识资源中心(Arid): 放线菌R2A14A-1的活性代谢产物分离及菌株鉴定

Arid

	放线菌R2A14A-1的活性代谢产物分离及菌株鉴定
其他题名	Identification of strain R2A14A-1 and its bioactive secondary metabolite
	王飞飞 1; 庹利 1; 刘佳萌 1; 刘少伟 1; 董艳萍 2; 蒋忠科 1; 夏曼 2; 李小俊 1; 陈川 1; 戴素娟 1; 许敏 1; 姜蓉 1; 孙承航 1
来源期刊	中国抗生素杂志
ISSN	1001-8689
出版年	2014
卷号	39 期号:10 页码:721-728
中文摘要	目的抗金黄色葡萄球菌化合物FFF-13的分离鉴定及其塔克拉玛干沙漠南麓来源产生菌R2A14A-1的初步鉴别。方法以琼脂平板扩散法和化合物紫外特征追踪R2A14A-1发酵液中抗金黄色葡萄球菌的amicoumacin类化合物,通过大孔吸附树脂和高效液相等色谱法建立空白培养基与菌株发酵液比对指纹图谱,通过比对分离获得活性次级代谢产物FFF-13,根据高分辨质谱、核磁共振氢谱和碳谱数据并结合文献确定化合物FFF-13的结构;综合菌株R2A14A-1的形态特征、培养特征、生理生化特性和16SrRNA基因序列分析对菌株R2A14A-1进行初步鉴定。结果化合物FFF-13为amicoumacin B,菌株R2A14A-1为拟诺卡菌属放线菌。结论菌株R2A14A-1为首个发现产生amicoumain类抗生素的拟诺卡菌株。
英文摘要	Objective Isolation and structural identification of secondary metabolite FFF-13 with anti-Staphylococcus aureus from the culture broth of strain R2A14A-1 isolated from the south edge of the Taklamakan Desert in Xinjiang province in China, and preliminary identification of its producing strain R2A14A-1. Methods Based on paper disc diffusion assay and UV spectra, HPLC fingerprint was established to identify the secondary metabolites by comparison of the fermentation broth with the blank culture medium after macroporous resin absorption and acetone water solution elution. Compound FFF-13 was identified as an active secondary metabolite and finally purified by HPLC. The chemical structure of FFF-13 was determined by HR-ESI-MS, NMR analysis and data comparisons with that of the related literature. Preliminary identification of strain R2A14A-1 was carried out by the method of comprehensive analysis of its phylogenetic relationships, combined with morphological, cultural and physiological characteristics. Results Compound FFF-13 was identified as amicoumacin B and the strain R2A14A-1 was affiliated with the genus Nocardiopsis. Conclusion To our best knowledge, it is the firstly reported that a Nocardiopsis strain can produce amicoumacin group antibiotics.
中文关键词	拟诺卡菌 ; 鉴别
英文关键词	Amicoumacin B 16SrRNA Nocardiopsis Amicoumacin B 16S rRNA Identification
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PHARMACOLOGY PHARMACY
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:5259499
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/232066
作者单位	1.中国医学科学院北京协和医学院医药生物技术研究所, 北京 100050, 中国; 2.中国药科大学生命科学与技术学院, 南京, 江苏 210009, 中国
推荐引用方式 GB/T 7714	王飞飞,庹利,刘佳萌,等. 放线菌R2A14A-1的活性代谢产物分离及菌株鉴定[J],2014,39(10):721-728.
APA	王飞飞.,庹利.,刘佳萌.,刘少伟.,董艳萍.,...&孙承航.(2014).放线菌R2A14A-1的活性代谢产物分离及菌株鉴定.中国抗生素杂志,39(10),721-728.
MLA	王飞飞,et al."放线菌R2A14A-1的活性代谢产物分离及菌株鉴定".中国抗生素杂志 39.10(2014):721-728.