Knowledge Resource Center for Ecological Environment in Arid Area
放线菌R2A14A-1的活性代谢产物分离及菌株鉴定 | |
其他题名 | Identification of strain R2A14A-1 and its bioactive secondary metabolite |
王飞飞1; 庹利1; 刘佳萌1; 刘少伟1; 董艳萍2; 蒋忠科1; 夏曼2; 李小俊1; 陈川1; 戴素娟1; 许敏1; 姜蓉1; 孙承航1 | |
来源期刊 | 中国抗生素杂志 |
ISSN | 1001-8689 |
出版年 | 2014 |
卷号 | 39期号:10页码:721-728 |
中文摘要 | 目的抗金黄色葡萄球菌化合物FFF-13的分离鉴定及其塔克拉玛干沙漠南麓来源产生菌R2A14A-1的初步鉴别。方法以琼脂平板扩散法和化合物紫外特征追踪R2A14A-1发酵液中抗金黄色葡萄球菌的amicoumacin类化合物,通过大孔吸附树脂和高效液相等色谱法建立空白培养基与菌株发酵液比对指纹图谱,通过比对分离获得活性次级代谢产物FFF-13,根据高分辨质谱、核磁共振氢谱和碳谱数据并结合文献确定化合物FFF-13的结构;综合菌株R2A14A-1的形态特征、培养特征、生理生化特性和16SrRNA基因序列分析对菌株R2A14A-1进行初步鉴定。结果化合物FFF-13为amicoumacin B,菌株R2A14A-1为拟诺卡菌属放线菌。结论菌株R2A14A-1为首个发现产生amicoumain类抗生素的拟诺卡菌株。 |
英文摘要 | Objective Isolation and structural identification of secondary metabolite FFF-13 with anti-Staphylococcus aureus from the culture broth of strain R2A14A-1 isolated from the south edge of the Taklamakan Desert in Xinjiang province in China, and preliminary identification of its producing strain R2A14A-1. Methods Based on paper disc diffusion assay and UV spectra, HPLC fingerprint was established to identify the secondary metabolites by comparison of the fermentation broth with the blank culture medium after macroporous resin absorption and acetone water solution elution. Compound FFF-13 was identified as an active secondary metabolite and finally purified by HPLC. The chemical structure of FFF-13 was determined by HR-ESI-MS, NMR analysis and data comparisons with that of the related literature. Preliminary identification of strain R2A14A-1 was carried out by the method of comprehensive analysis of its phylogenetic relationships, combined with morphological, cultural and physiological characteristics. Results Compound FFF-13 was identified as amicoumacin B and the strain R2A14A-1 was affiliated with the genus Nocardiopsis. Conclusion To our best knowledge, it is the firstly reported that a Nocardiopsis strain can produce amicoumacin group antibiotics. |
中文关键词 | 拟诺卡菌 ; 鉴别 |
英文关键词 | Amicoumacin B 16SrRNA Nocardiopsis Amicoumacin B 16S rRNA Identification |
语种 | 中文 |
国家 | 中国 |
收录类别 | CSCD |
WOS类目 | PHARMACOLOGY PHARMACY |
WOS研究方向 | Pharmacology & Pharmacy |
CSCD记录号 | CSCD:5259499 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/232066 |
作者单位 | 1.中国医学科学院北京协和医学院医药生物技术研究所, 北京 100050, 中国; 2.中国药科大学生命科学与技术学院, 南京, 江苏 210009, 中国 |
推荐引用方式 GB/T 7714 | 王飞飞,庹利,刘佳萌,等. 放线菌R2A14A-1的活性代谢产物分离及菌株鉴定[J],2014,39(10):721-728. |
APA | 王飞飞.,庹利.,刘佳萌.,刘少伟.,董艳萍.,...&孙承航.(2014).放线菌R2A14A-1的活性代谢产物分离及菌株鉴定.中国抗生素杂志,39(10),721-728. |
MLA | 王飞飞,et al."放线菌R2A14A-1的活性代谢产物分离及菌株鉴定".中国抗生素杂志 39.10(2014):721-728. |
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