干旱区生态环境知识资源中心(Arid): 不同草原类型针茅根部内生真菌群落结构

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	不同草原类型针茅根部内生真菌群落结构
其他题名	Endophytic fungal communities of Stipa sproots in different types of steppes in northern China
	张少鹏 1; 胥婷 1; 杨丽强 1; 宋宇 2; 李新宇 1; 张惠文 1
来源期刊	应用生态学报
ISSN	1001-9332
出版年	2014
卷号	25 期号:12 页码:3475-3482
中文摘要	为了解我国北方不同草原类型中针茅根部内生真菌的群落结构及多样性变化,从新疆、甘肃、内蒙古3省(区)选择了6种不同草原类型(亚高山草甸、高山草甸、戈壁、荒漠草原、典型草原和草甸草原),进行针茅根部组织内生真菌的研究.共分离得到针茅根部内生真菌213株,根据序列的相似性(以97%为阈值),共获得51个真菌分类操作单元(OTUs),覆盖了4门7纲23科27属.在门的水平上子囊菌门真菌为绝对优势菌群,占分离真菌总数的93. 4%,在各草原类型中均有分布; 6种草原类型中针茅根部内生真菌的优势属差别较大,仅子囊菌门的镰孢菌属为各草原类型共有优势属,占分离真菌总数的41. 3%,亚高山草甸的微结节霉属、高山草甸的Saccharicola和短梗霉属、戈壁的弯孢属和根霉属以及草甸草原的木霉属,为各草原类型中针茅根部内生真菌的优势属.高山草甸针茅根部内生真菌群落覆盖的门和属最多,Margalef丰富度指数和香农多样性指数最高,均匀度指数仅次于荒漠草原;而荒漠草原的Margalef丰富度指数最低,典型草原的多样性指数和均匀度指数最低.高山草甸和荒漠草原的内生真菌群落结构与其他草原类型之间的相似性系数都较低,分别为0. 12~ 0. 25和0. 13~ 0. 22,其他几种草原类型之间相似性相对较高,尤其是典型草原和草甸草原之间相似性系数为0. 60.冗余分析(RDA)表明,海拔和纬度是影响6种草原类型中针茅根部内生真菌群落结构变化的主要环境因子.
英文摘要	In order to explore the endophytic fungal communities of Stipa sp. roots in different types of steppes in the north of China,root tissues of Stipa sp. were collected from six different types of steppes,i. e.,subalpine meadow,alpine meadow,Gobi desert,desert steppe,typical steppe,and meadow steppe across Xinjiang,Gansu and Inner Mongolia. A total of 213 isolates were obtained and sequenced,which were divided into 51 operational taxonomic units (OTUs) at the 97% similarity threshold and classified to 4 phyla,7 classes,23 families and 27 genera. Ascomycota was found to be the predominant flora (93. 4% of the total isolates) at the level of phylum and distributed in all the six steppe types. The dominant genera other than Fusarium (41. 3 of the total isolates) in the six steppe types were different,such as Microdochium in subalpine meadow,Saccharicola and Aureobasidium in alpine meadow,Curvularia and Rhizopus in Gobi desert and Trichoderma in typical steppe. The endophytic fungal community from the alpine meadow contained the most abundant groups at the level of genus and phylum,and had the highest Margalef index,Shannon index and second highest evenness index after the desert steppe. By contrast,the lowest Margalef index was observed in the desert steppe and the lowest Shannon index and evenness index were found in the typical steppe. Furthermore,endophytic fungal communities from alpine meadow and desert steppe showed low similarity coefficients (0. 12-0. 25 and 0. 13-0. 22,respectively) with other steppe types. However,the similarity coefficients among the other four steppe types were relatively high,especially between the typical steppe and the meadow steppe (0. 60). Redundancy analysis(RDA) showed that the altitude and latitude were the main environmental factors affecting endophytic fungal community distribution in the roots of Stipa sp. in the six steppe types.
中文关键词	针茅 ; 内生真菌 ; 多样性指数 ; 相似性系数 ; 冗余分析(RDA)
英文关键词	Stipa sp. endophytic fungi diversity index similarity coefficient redundancy analysis(RDA)
语种	中文
国家	中国
收录类别	CSCD
WOS类目	FISHERIES
WOS研究方向	Fisheries
CSCD记录号	CSCD:5307182
来源机构	中国科学院沈阳应用生态研究所
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231996
作者单位	1.中国科学院沈阳应用生态研究所, 森林与土壤生态国家重点实验室, 沈阳, 辽宁 110164, 中国; 2.辽东学院, 丹东, 辽宁 118003, 中国
推荐引用方式 GB/T 7714	张少鹏,胥婷,杨丽强,等. 不同草原类型针茅根部内生真菌群落结构[J]. 中国科学院沈阳应用生态研究所,2014,25(12):3475-3482.
APA	张少鹏,胥婷,杨丽强,宋宇,李新宇,&张惠文.(2014).不同草原类型针茅根部内生真菌群落结构.应用生态学报,25(12),3475-3482.
MLA	张少鹏,et al."不同草原类型针茅根部内生真菌群落结构".应用生态学报 25.12(2014):3475-3482.