干旱区生态环境知识资源中心(Arid): SPE - HPLC 法测定马破伤风免疫球蛋白F(ab’)_2中Triton X

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	SPE - HPLC 法测定马破伤风免疫球蛋白F(ab’)_2中Triton X - 100残留量
其他题名	Determination of Triton X - 100 residues in the equine antitetanus immunoglobulin F(ab’)_2 by SPE - HPLC
	白文莉 1; 高家敏 2; 余振喜 2
来源期刊	药物分析杂志
ISSN	0254-1793
出版年	2014
卷号	34 期号:7 页码:1251-1255
中文摘要	目的:建立固相萃取高效液相色谱(SPE-HPLC)法测定马破伤风免疫球蛋白F(ab’)_2中灭活剂Triton X-100的残留量。方法:采用Oasis 固相萃取小柱对样品进行预处理,以水和5% 甲醇为淋洗剂,以甲醇为洗脱剂,收集甲醇洗脱物用于 HPLC 分析。采用ZORBAX Extend-C_(18)色谱柱(4.6mm * 150mm,5mum),流动相为甲醇- 水(80∶ 20),流速1.0mL·min~(-1),检测波长230nm,柱温30℃,进样量10muL。结果:Triton X-100浓度在0.56~ 55.34mug·mL~(-1)范围内,与峰面积线性关系良好(r = 0.9998);检测限(S /N = 3)为2.03ng,定量限(S /N = 10)为4.06ng;提取回收率为91.8%。结论:本方法操作简便、准确、重复性好,可用于Triton X-100的残留量测定。
英文摘要	Objective: To establish a SPE - HPLC method for the determination of Triton X - 100 residues in equine antitetanus immunoglobulin F(ab’)_2. Methods: Samples were pre - treated on Oasis HLB SPE column, then cleaned - up by water and 5% methanol,and finally extracted by methanol. The analysis of extracts was performed on a ZORBAX Extend - C_(18) column(4. 6 mm * 150 mm,5 mum) with a mobile phase consisting of methanol - water(80∶ 20,v /v) at a flow rate of 1. 0 mL·min~(-1). The column temperature was set at 30 ℃ and the injection volume was 10 muL. Triton X - 100 was detected at the wavelength of 230 nm with a DAD detection. Results: The established HPLC method resulted in sharp and symmetric peaks of Triton X - 100,whereas the calibration curve of Triton X - 100 displayed a good linearity within the concentration of 0. 56 - 55. 34 mug·mL~(-1) (r = 0. 9998). The LOD and LOQ were 2. 03 ng and 4. 06 ng, respectively. Specificity,precision,accuracy, stability and robustness were acceptable. The extraction recovery was 91. 8%. Conclusion: The results indicate that the established method is convenient,accurate and reproducible,which can be used in the analysis of Triton X - 100 residues in equine antitetanus immunoglobulin F(ab’)_2.
中文关键词	马破伤风免疫球蛋白F(ab’)_2 ; 灭活剂 ; 裂解剂 ; 辛基苯氧基聚2 - 乙氧基乙醇 ; 杂质残留检测 ; 固相萃取 ; 高效液相色谱法
英文关键词	Triton X - 100 equine antitetanus immunoglobulin F(ab’)_2 Triton X - 100 inactivator split agent octylphenoxypolyethoxyethanol impurity residue detection SPE HPLC
语种	中文
国家	中国
收录类别	CSCD
WOS类目	PHARMACOLOGY PHARMACY
WOS研究方向	Pharmacology & Pharmacy
CSCD记录号	CSCD:5198723
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231971
作者单位	1.昆明市食品药品检验所, 昆明, 云南 650032, 中国; 2.中国食品药品检定研究院, 北京 100050, 中国
推荐引用方式 GB/T 7714	白文莉,高家敏,余振喜. SPE - HPLC 法测定马破伤风免疫球蛋白F(ab’)_2中Triton X - 100残留量[J],2014,34(7):1251-1255.
APA	白文莉,高家敏,&余振喜.(2014).SPE - HPLC 法测定马破伤风免疫球蛋白F(ab’)_2中Triton X - 100残留量.药物分析杂志,34(7),1251-1255.
MLA	白文莉,et al."SPE - HPLC 法测定马破伤风免疫球蛋白F(ab’)_2中Triton X - 100残留量".药物分析杂志 34.7(2014):1251-1255.