干旱区生态环境知识资源中心(Arid): 温度对荒漠昆虫小胸鳖甲幼虫肌动蛋白基因表达的影响

Arid

	温度对荒漠昆虫小胸鳖甲幼虫肌动蛋白基因表达的影响
其他题名	Effect of Temperature on the Expression of beta - actin Gene in the Desert Insect Microdera punctipennis ( Coleoptera : Tenebriodae) Larvae
	肖荣; 刘小静; 石萌; 马纪
来源期刊	新疆农业科学
ISSN	1001-4330
出版年	2014
卷号	51 期号:8 页码:1488-1495
中文摘要	【目的】肌动蛋白beta-actin是应用实时荧光定量PCR检测基因表达的常用内参基因，测定和分析荒漠昆虫小胸鳖甲幼虫中beta-actin基因表达的稳定性，判断其是否可作为内参基因。【方法】选取不同龄期的小胸鳖甲幼虫经过42、5、10和15°C处理不同时间后，利用实时荧光定量PCR检测beta-actin基因的表达，以基因绝对拷贝数的对数进行单因素方差分析。【结果】小胸鳖甲幼虫在不同龄期beta-actin的表达有显著差异；1龄幼虫在不同温度处理后，beta - actin的表达都有显著升高；4龄幼虫在不同温度处理后beta - actin的表达基本上没有变化；7龄幼虫在10、15和42°C条件下beta-actin的表达都显著升高，但5oC处理没有影响。【结论】小胸鳖甲幼虫的beta-actin基因具有发育阶段性差异性表达特点，温度对不同龄期幼虫的beta-actin基因表达的影响不同,beta-actin基因可能不适合在小胸鳖甲幼虫基因表达分析中作为内参基因。
英文摘要	【Objective 】beta - actin gene is commonly used as a reference gene in real time fluorescence quantitative PCR ( qRT - PCR) for assaying gene expression. The present study aims at evaluating if beta- actin gene is appropriate for being used as a reference gene in the larvae of Microdera punctipenis through analysis of the expression stability of beta - actin gene under different temperatures.【Method 】M. punctipenis larvae of different instars were treated at 42°C , 5oC, l0oC, 15oC respectively for various hours, and the expression of beta-actin gene was then determined by qRT - PCR. The data were converted to the logarithm of the absolute copies of the gene, and then statistically analyzed with one way ANOVA. [ Result 】The results showed a significant difference in the expression level of beta- actin gene in M. punctipennis larvae from the 1st to the 7th instars. The expression of beta - actin gene in the 1 st instar after being treated at different temperatures was significantly increased. However, there was no significant change in the expression of beta - actin gene in the 4th instar after temperature treatment. For the 7th instar the expression of beta - actin gene was up - regulated after the larvae were treated at 10oC, 15oC,and 42oC respectively, but 5oC had no influence in the gene expression.【Con- clusion 】The expression of beta - actin gene in M. punctipenis larvae was developmentally differential. The influence of temperature in the expression of beta - actin gene in M. punctipenis larvae varies. beta - actin gene may not to be used as an appropriate reference gene in the gene expression analysis in M. punctipenis larvae.
中文关键词	小胸鳖甲 ; 荧光定量PCR ; 基因表达 ; 温度处理
英文关键词	beta-actin Microdera punctipennis real - time PCR beta- actin expression temperature treatment
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRICULTURE MULTIDISCIPLINARY
WOS研究方向	Agriculture
CSCD记录号	CSCD:5281694
来源机构	新疆大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231944
作者单位	新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 乌鲁木齐, 新疆 830046, 中国
推荐引用方式 GB/T 7714	肖荣,刘小静,石萌,等. 温度对荒漠昆虫小胸鳖甲幼虫肌动蛋白基因表达的影响[J]. 新疆大学,2014,51(8):1488-1495.
APA	肖荣,刘小静,石萌,&马纪.(2014).温度对荒漠昆虫小胸鳖甲幼虫肌动蛋白基因表达的影响.新疆农业科学,51(8),1488-1495.
MLA	肖荣,et al."温度对荒漠昆虫小胸鳖甲幼虫肌动蛋白基因表达的影响".新疆农业科学 51.8(2014):1488-1495.