干旱区生态环境知识资源中心(Arid): 生物炭对灰漠土和风沙土土壤微生物多样性及与氮素相关微生物功能的影响

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	生物炭对灰漠土和风沙土土壤微生物多样性及与氮素相关微生物功能的影响
其他题名	Effects of Biochar on Soil Microbial Diversity and Function Related with N Transformation in Grey Desert Soil and Aeolian Sandy Soil in Xinjiang
	顾美英 1; 徐万里 2; 唐光木 2; 葛春辉 2; 马海刚 2
来源期刊	新疆农业科学
ISSN	1001-4330
出版年	2014
卷号	51 期号:5 页码:926-934
中文摘要	【目的】研究生物炭对新疆灰漠土和风沙土土壤微生物多样性及与氮素转化相关功能菌和功能酶的影响。【方法】室内恒温箱进行70 d培养。【结果】(1)在灰漠土上,不施氮肥的对照与生物炭处理对细菌数量无显著影响(P > 0. 05);与单施氮肥的对照相比,生物炭配施氮肥显著增加了24. 49%细菌数量(P < 0. 05);但无论施氮与否,生物炭对放线菌和真菌数量均呈降低趋势(P>0.05)。在风沙土上,无论施生物炭与否,对细菌数量无显著影响(P>0.05);但生物炭对放线菌和真菌数量影响差异显著(P<0.05),呈增加趋势。(2)培养结束后土壤Biolog碳源利用测定显示,施用生物炭两种土壤微生物多样性有差异(P <0.05)。单施生物炭和生物炭配施氮肥均可提高两种土壤微生物群落的丰富度。(3)在灰漠土上,单施生物炭和生物炭配施氮肥处理对自生固氮菌、氨化细菌、亚硝化细菌均有显著促进作用(P<0.05)。在风沙土上除亚硝化细菌差异不显著外,单施生物炭和生物炭配施氮肥对自生固氮菌和氨化细菌有显著促进作用(P < 0. 05)。两种土壤上单施生物炭和生物炭配施氮肥处理反硝化细菌数量均呈降低趋势。(4)在灰漠土上,单施生物炭和生物炭配施氮肥处理对脲酶有显著抑制作用(P <0.05),对蛋白酶有显著促进作用(P < 0. 05);在风沙土上,这两种处理对脲酶无明显影响,但对蛋白酶有显著抑制作用(P < 0.05)。【结论】生物炭能提高新疆灰漠土和风沙土与氮素转化相关的土壤微生物多样性,生物炭配施氮肥优于单施生物炭,灰漠土优于风沙土。
英文摘要	【Objective 】This project aims to study the effects of biochar on soil microbial diversity and functional bacteria and enzymes related with N transformation in grey desert soil and aeolian sandy soil in Xinjiang. 【Method】Incubation method for 70 days.【Result】(1) In grey desert soil,compared with no fertilizer treatment, the numbers of bacteria of single biochar treatment were not significantly different (P > 0. 05). Compared with single nitrogen fertilization treatment, nitrogen fertilization and biochar treatment were increased significantly by 24. 49% (P < 0. 05). Whether nitrogen applied or not, effects of biochar on the numbers of actinomycetes and fungi were decreased (P >0.05). In aeolian sandy soil, whether biochar applied or not, the numbers of bacteria were not significantly different (P >0. 05). But effects of biochar on the numbers of actinomycetes and fungi were significantly increased (P <0. 05). (2) After incubation time, carbon utilizations of soil microbial community showed that soil microbial diversities were significantly different by applying biochar in both kinds of the soils (P <0. 05). Single biochar treatment and nitrogen fertilization and biochar treatment could improve microbial community richness index. (3)The amounts of azotobacter,ammoniation and nitrobacteria in single biochar treatment and nitrogen fertilization and biochar treatment were increased significantly (P <0. 05) in grey desert soil. Except for the amount of nitrobacteria, the amounts of azotobacter and ammoniation in single biochar treatment and nitrogen fertilization and biochar treatment were increased significantly in aeolian sandy soil (P <0.05). But the amounts of denitrifying in single biochar treatment and nitrogen fertilization and biochar treatment were decreased in two soils. (4) Urease activities were obviously decreased, but protease activities were obviously increased by single biochar treatment and nitrogen fertilization and biochar treatment in grey desert soil (P <0. 05). Urease activities were not significantly different,but protease activities were obviously increased by single biochar treatment and nitrogen fertilization and biochar treatment in aeolian sandy soil (P <0. 05).【Conclusion】In brief, biochar could improve soil microbial diversities related with N transfor mation in grey desert soil and aeolian sandy soil. Result of nitrogen fertilization and biochar treatment was better than the single biochar treatment and grey desert soil was better than aeolian sandy soil.
中文关键词	生物炭 ; 微生物 ; 氮素转化 ; 多样性 ; 灰漠土 ; 风沙土
英文关键词	biochar soil microbe N transformation diversity grey desert soil aeolian sandy soil
语种	中文
国家	中国
收录类别	CSCD
WOS类目	AGRONOMY
WOS研究方向	Agriculture
CSCD记录号	CSCD:5205569
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/231942
作者单位	1.新疆农业科学院微生物应用研究所/绿洲养分与水土资源高效利用重点实验室, 乌鲁木齐, 新疆 830092, 中国; 2.新疆农业科学院土壤肥料与农业节水研究所, 乌鲁木齐, 新疆 830092, 中国
推荐引用方式 GB/T 7714	顾美英,徐万里,唐光木,等. 生物炭对灰漠土和风沙土土壤微生物多样性及与氮素相关微生物功能的影响[J],2014,51(5):926-934.
APA	顾美英,徐万里,唐光木,葛春辉,&马海刚.(2014).生物炭对灰漠土和风沙土土壤微生物多样性及与氮素相关微生物功能的影响.新疆农业科学,51(5),926-934.
MLA	顾美英,et al."生物炭对灰漠土和风沙土土壤微生物多样性及与氮素相关微生物功能的影响".新疆农业科学 51.5(2014):926-934.